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The map label for this gene is liaR [H]

Identifier: 116872424

GI number: 116872424

Start: 1026147

End: 1026785

Strand: Direct

Name: liaR [H]

Synonym: lwe1006

Alternate gene names: 116872424

Gene position: 1026147-1026785 (Clockwise)

Preceding gene: 116872423

Following gene: 116872425

Centisome position: 36.46

GC content: 35.99

Gene sequence:

>639_bases
ATGATAAAAGTATTACTTGTTGATGATCACGAAATGGTACGTATAGGTGTCTCAGCTTATCTTTCAGTACAAGATGACAT
GGAAGTAGTCGGCGAGGCAGAAAATGGACGAGAAGGTGCCGATATGGCCTTAGAACTCCGTCCAGATATCATCTTAATGG
ATTTAGTTATGGATGAAATGGATGGTATCGAAGCGACTAAAGAAATCATGCAAAAATGGAAAGAAGCAAAAATCATTATC
GTCACAAGTTTTATTGATGATGAAAAAGTTTACCCAGCACTAGAAGCTGGAGCGAGCAGTTACATGTTAAAAACATCCAC
AGCAAGCGAAATTGCCGATGCGATTCGTGCTACATATGGTGGGGACTCTGTGCTTGAACCAGAAGTAACTGGTAAAATGA
TGCAACGATTAACCGCTAAACCAGAAAAAAACTTACATGATGATTTAACTAATCGTGAAAATGAAATTCTCTTATTAATC
GCAGAAGGTAAATCTAATCAAGAAATTGCAGATGAACTTTTTATCACTTTGAAAACAGTAAAAACGCATGTAAGTAATAT
TTTATCAAAACTTGATGTACAAGACCGTACACAAGCTGCGATTTATGCATTTAAACATGATTTAGTAGAAAAAAAGTAG

Upstream 100 bases:

>100_bases
CTGAGTTCGGTGGCACTATCAAAATAATTAGTTTCCCCGGAAGAGGAACTAGCGTAGAAATAAAAATCCCACTTGTTGCG
AAGAAGGACGTGGAAAGCCA

Downstream 100 bases:

>100_bases
CAAGGAGGTAACCAACACATGAAAGAAGGTTTTGCAGTTATCGGTCTTGGGCGTTTTGGCGGGAGCATTTGTCGTTCACT
TGTTGAACAAGGAATGGAAG

Product: LuxR family DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MIKVLLVDDHEMVRIGVSAYLSVQDDMEVVGEAENGREGADMALELRPDIILMDLVMDEMDGIEATKEIMQKWKEAKIII
VTSFIDDEKVYPALEAGASSYMLKTSTASEIADAIRATYGGDSVLEPEVTGKMMQRLTAKPEKNLHDDLTNRENEILLLI
AEGKSNQEIADELFITLKTVKTHVSNILSKLDVQDRTQAAIYAFKHDLVEKK

Sequences:

>Translated_212_residues
MIKVLLVDDHEMVRIGVSAYLSVQDDMEVVGEAENGREGADMALELRPDIILMDLVMDEMDGIEATKEIMQKWKEAKIII
VTSFIDDEKVYPALEAGASSYMLKTSTASEIADAIRATYGGDSVLEPEVTGKMMQRLTAKPEKNLHDDLTNRENEILLLI
AEGKSNQEIADELFITLKTVKTHVSNILSKLDVQDRTQAAIYAFKHDLVEKK
>Mature_212_residues
MIKVLLVDDHEMVRIGVSAYLSVQDDMEVVGEAENGREGADMALELRPDIILMDLVMDEMDGIEATKEIMQKWKEAKIII
VTSFIDDEKVYPALEAGASSYMLKTSTASEIADAIRATYGGDSVLEPEVTGKMMQRLTAKPEKNLHDDLTNRENEILLLI
AEGKSNQEIADELFITLKTVKTHVSNILSKLDVQDRTQAAIYAFKHDLVEKK

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=205, Percent_Identity=35.609756097561, Blast_Score=116, Evalue=9e-28,
Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=30.952380952381, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787473, Length=204, Percent_Identity=37.2549019607843, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1790102, Length=209, Percent_Identity=30.1435406698565, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786747, Length=209, Percent_Identity=28.7081339712919, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1788712, Length=190, Percent_Identity=27.3684210526316, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI87082052, Length=104, Percent_Identity=36.5384615384615, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788550, Length=102, Percent_Identity=34.3137254901961, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23686; Mature: 23686

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVLLVDDHEMVRIGVSAYLSVQDDMEVVGEAENGREGADMALELRPDIILMDLVMDEM
CEEEEEECCCCEEEEEHHHEEEECCCHHHHCCCCCCCCCCCEEEEECCCEEEHHHHHHHH
DGIEATKEIMQKWKEAKIIIVTSFIDDEKVYPALEAGASSYMLKTSTASEIADAIRATYG
CCHHHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHCCCCCEEEECCCHHHHHHHHHHHCC
GDSVLEPEVTGKMMQRLTAKPEKNLHDDLTNRENEILLLIAEGKSNQEIADELFITLKTV
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
KTHVSNILSKLDVQDRTQAAIYAFKHDLVEKK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKVLLVDDHEMVRIGVSAYLSVQDDMEVVGEAENGREGADMALELRPDIILMDLVMDEM
CEEEEEECCCCEEEEEHHHEEEECCCHHHHCCCCCCCCCCCEEEEECCCEEEHHHHHHHH
DGIEATKEIMQKWKEAKIIIVTSFIDDEKVYPALEAGASSYMLKTSTASEIADAIRATYG
CCHHHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHCCCCCEEEECCCHHHHHHHHHHHCC
GDSVLEPEVTGKMMQRLTAKPEKNLHDDLTNRENEILLLIAEGKSNQEIADELFITLKTV
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
KTHVSNILSKLDVQDRTQAAIYAFKHDLVEKK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]