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The map label for this gene is ypqE [H]

Identifier: 116872419

GI number: 116872419

Start: 1021990

End: 1022481

Strand: Direct

Name: ypqE [H]

Synonym: lwe1001

Alternate gene names: 116872419

Gene position: 1021990-1022481 (Clockwise)

Preceding gene: 116872418

Following gene: 116872420

Centisome position: 36.32

GC content: 33.74

Gene sequence:

>492_bases
ATGTTTAAAAAATTCTTGAATAAAAGTAAAAGTGAAGTAGTATTTGCGCATGTAACTGGAAAACTTATTGCTTTAGAAGA
TGTTCCAGATCCAGTTTTTAATCAAAAAATGATGGGTGAGGGAATCGCAATTAAGCCAGAAACAGGTACAATTGTTGCTC
CAGTAGATGGGAAAATTATTCAACTTGCAGAAACAAAACACGCTTTTGGCATTCGTACTGATATGGGACAAGAAATTCTT
GTACATATTGGTTTAGAAACAGTAGCATTAAATGGAGAAGGTTTTAATGTTCTTGTAAACGTAGGCGATAAAGTCAAAGT
CGGAGATCCTATCGTGGAAGCGGATTTTGATTTTATTGAAAAAAATGCTGCAAGTACTGTTGTTCCAATGGTCGTAACTA
ATAGTTCAGAAGGCCAATATGATTTTGCTTTTGAAGATGTATCCAAAACTGTAGCAGGAGAAACAGAAGTATTTACAACG
AAATTAAAATAA

Upstream 100 bases:

>100_bases
ATGAAAAAGTTCAAGTTAATCTTGATACTTTCTTCGTGCCTTTTCTTCTTCAAAAAGGTAGAATAGAAGTTGACTTATGA
ATTTGCTAGGAGGAAGATGG

Downstream 100 bases:

>100_bases
TCAAAGTAAAAAATCCATGATAAGAGTAATTTTCTTTTCATGGATTTTTTAGTATTCATTTATGTATTAAAAGGGTAAAA
TGAAGATAATTAGTTAATAA

Product: PTS system, glucose-specific, IIA component, putative

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIA component [H]

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MFKKFLNKSKSEVVFAHVTGKLIALEDVPDPVFNQKMMGEGIAIKPETGTIVAPVDGKIIQLAETKHAFGIRTDMGQEIL
VHIGLETVALNGEGFNVLVNVGDKVKVGDPIVEADFDFIEKNAASTVVPMVVTNSSEGQYDFAFEDVSKTVAGETEVFTT
KLK

Sequences:

>Translated_163_residues
MFKKFLNKSKSEVVFAHVTGKLIALEDVPDPVFNQKMMGEGIAIKPETGTIVAPVDGKIIQLAETKHAFGIRTDMGQEIL
VHIGLETVALNGEGFNVLVNVGDKVKVGDPIVEADFDFIEKNAASTVVPMVVTNSSEGQYDFAFEDVSKTVAGETEVFTT
KLK
>Mature_163_residues
MFKKFLNKSKSEVVFAHVTGKLIALEDVPDPVFNQKMMGEGIAIKPETGTIVAPVDGKIIQLAETKHAFGIRTDMGQEIL
VHIGLETVALNGEGFNVLVNVGDKVKVGDPIVEADFDFIEKNAASTVVPMVVTNSSEGQYDFAFEDVSKTVAGETEVFTT
KLK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788757, Length=124, Percent_Identity=47.5806451612903, Blast_Score=123, Evalue=6e-30,
Organism=Escherichia coli, GI1786894, Length=156, Percent_Identity=39.7435897435897, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1790159, Length=124, Percent_Identity=39.5161290322581, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR001127 [H]

Pfam domain/function: PF00358 PTS_EIIA_1 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17630; Mature: 17630

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKFLNKSKSEVVFAHVTGKLIALEDVPDPVFNQKMMGEGIAIKPETGTIVAPVDGKII
CCHHHHCCCCCCEEEEEECCEEEEEECCCCHHHCHHHHCCCEEEECCCCEEEECCCCCEE
QLAETKHAFGIRTDMGQEILVHIGLETVALNGEGFNVLVNVGDKVKVGDPIVEADFDFIE
EEECCCCCCEECCCCCCEEEEEECEEEEEECCCCEEEEEECCCEEEECCCEEECCHHHHH
KNAASTVVPMVVTNSSEGQYDFAFEDVSKTVAGETEVFTTKLK
CCCCCEEEEEEEECCCCCCEEEEHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure
MFKKFLNKSKSEVVFAHVTGKLIALEDVPDPVFNQKMMGEGIAIKPETGTIVAPVDGKII
CCHHHHCCCCCCEEEEEECCEEEEEECCCCHHHCHHHHCCCEEEECCCCEEEECCCCCEE
QLAETKHAFGIRTDMGQEILVHIGLETVALNGEGFNVLVNVGDKVKVGDPIVEADFDFIE
EEECCCCCCEECCCCCCEEEEEECEEEEEECCCCEEEEEECCCEEEECCCEEECCHHHHH
KNAASTVVPMVVTNSSEGQYDFAFEDVSKTVAGETEVFTTKLK
CCCCCEEEEEEEECCCCCCEEEEHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]