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The map label for this gene is sigB [H]

Identifier: 116872297

GI number: 116872297

Start: 908036

End: 908815

Strand: Direct

Name: sigB [H]

Synonym: lwe0877

Alternate gene names: 116872297

Gene position: 908036-908815 (Clockwise)

Preceding gene: 116872296

Following gene: 116872298

Centisome position: 32.27

GC content: 37.18

Gene sequence:

>780_bases
ATGCCAAAAGTATCTCAACCTGATAAAGAAGCAAAAGAAAAAGTATATATTTGGATTGCCGCTTATCAAGAAAACGGCGA
CCAAGATGCCCAATATAATTTAGTGGTTCATTATAAAAATTTAGTAGAATCTATTGCACGCAAGTATTCGCAAGGTAAAT
CTTTTCATGAGGATTTAGTGCAAGTTGGAAATATTGGTTTACTAGGTGCTATTAGACGTTATGATGCTACTTTTGGGAAA
AGCTTTGAAGCATTTGCTGTGCCAACTATTGTTGGTGAAATTAAACGTTTTTTACGCGATAAAACATGGAGCGTTCATGT
ACCGCGCCGAATTAAAGAATTAGGTCCAAAAATTAAGAATGCTGTGGAAGAGTTAACAAGAGAATTACAAAGCTCTCCTC
AAATTAGCGATATTGCTGATTTTATCGGCGTCACTGAAGAAGAAGTTTTAGAAGCAATGGAAATGGGGAAAAGTTACCAA
GCACTTTCTGTAGACCATTCAATTGAAGCTGATTCAGATGGAAGTACGATTACGTTACTTGATGTTGTAGGAGGTACGGA
TGATGGATTCGAACGTGTGAATCAACGTATGCTTCTAGAGAAAGTTCTCCCTGTCTTAGATGAGCGAGAACAGATGATTT
TGCAATATACATTTATTGAAAATCGCAGTCAAAAAGAAACTGGGGAACTACTCGATATATCACAAATGCATGTGTCTAGA
ATCCAACGTCAAGCTATCAAAAAACTTCGAGAGGCGTTGCAGAATGAGGAAGTGGAGTAA

Upstream 100 bases:

>100_bases
TTTTTAATTGAAACATTAATGGATGATGTGAAACTTTATTACGATGAAGGGGTTTCTGTCGTAATGACCAAATATATTAA
TGAAAAGCAGGTGGAGGAGA

Downstream 100 bases:

>100_bases
ATGAACAAGGCAGTTGAATCAAATAATTTATTTGTATTTCAACGTTCTAAAGCATTACAACAATACTGCGGGGATGTTTA
TTTTACCCATCAAGACAAAA

Product: RNA polymerase sigma factor SigB

Products: NA

Alternate protein names: General stress protein 84; GSP84; Sigma-37 [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MPKVSQPDKEAKEKVYIWIAAYQENGDQDAQYNLVVHYKNLVESIARKYSQGKSFHEDLVQVGNIGLLGAIRRYDATFGK
SFEAFAVPTIVGEIKRFLRDKTWSVHVPRRIKELGPKIKNAVEELTRELQSSPQISDIADFIGVTEEEVLEAMEMGKSYQ
ALSVDHSIEADSDGSTITLLDVVGGTDDGFERVNQRMLLEKVLPVLDEREQMILQYTFIENRSQKETGELLDISQMHVSR
IQRQAIKKLREALQNEEVE

Sequences:

>Translated_259_residues
MPKVSQPDKEAKEKVYIWIAAYQENGDQDAQYNLVVHYKNLVESIARKYSQGKSFHEDLVQVGNIGLLGAIRRYDATFGK
SFEAFAVPTIVGEIKRFLRDKTWSVHVPRRIKELGPKIKNAVEELTRELQSSPQISDIADFIGVTEEEVLEAMEMGKSYQ
ALSVDHSIEADSDGSTITLLDVVGGTDDGFERVNQRMLLEKVLPVLDEREQMILQYTFIENRSQKETGELLDISQMHVSR
IQRQAIKKLREALQNEEVE
>Mature_258_residues
PKVSQPDKEAKEKVYIWIAAYQENGDQDAQYNLVVHYKNLVESIARKYSQGKSFHEDLVQVGNIGLLGAIRRYDATFGKS
FEAFAVPTIVGEIKRFLRDKTWSVHVPRRIKELGPKIKNAVEELTRELQSSPQISDIADFIGVTEEEVLEAMEMGKSYQA
LSVDHSIEADSDGSTITLLDVVGGTDDGFERVNQRMLLEKVLPVLDEREQMILQYTFIENRSQKETGELLDISQMHVSRI
QRQAIKKLREALQNEEVE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma B is not essential for sporulation; rather it is required for maximal expression of ctc and csbA which are transcr

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=200, Percent_Identity=29.5, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789098, Length=260, Percent_Identity=25.7692307692308, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789448, Length=236, Percent_Identity=24.5762711864407, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789871, Length=244, Percent_Identity=28.6885245901639, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014288
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29529; Mature: 29398

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKVSQPDKEAKEKVYIWIAAYQENGDQDAQYNLVVHYKNLVESIARKYSQGKSFHEDLV
CCCCCCCCHHHHCEEEEEEEEEECCCCCCCEEEHEEHHHHHHHHHHHHHHCCCHHHHHHH
QVGNIGLLGAIRRYDATFGKSFEAFAVPTIVGEIKRFLRDKTWSVHVPRRIKELGPKIKN
HHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHCHHHHH
AVEELTRELQSSPQISDIADFIGVTEEEVLEAMEMGKSYQALSVDHSIEADSDGSTITLL
HHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEEEE
DVVGGTDDGFERVNQRMLLEKVLPVLDEREQMILQYTFIENRSQKETGELLDISQMHVSR
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
IQRQAIKKLREALQNEEVE
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PKVSQPDKEAKEKVYIWIAAYQENGDQDAQYNLVVHYKNLVESIARKYSQGKSFHEDLV
CCCCCCCHHHHCEEEEEEEEEECCCCCCCEEEHEEHHHHHHHHHHHHHHCCCHHHHHHH
QVGNIGLLGAIRRYDATFGKSFEAFAVPTIVGEIKRFLRDKTWSVHVPRRIKELGPKIKN
HHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHCHHHHH
AVEELTRELQSSPQISDIADFIGVTEEEVLEAMEMGKSYQALSVDHSIEADSDGSTITLL
HHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEEEE
DVVGGTDDGFERVNQRMLLEKVLPVLDEREQMILQYTFIENRSQKETGELLDISQMHVSR
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
IQRQAIKKLREALQNEEVE
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3016731; 3027048; 2170324; 9384377; 9298659 [H]