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The map label for this gene is nrtB [H]

Identifier: 116689996

GI number: 116689996

Start: 2194042

End: 2194917

Strand: Direct

Name: nrtB [H]

Synonym: Bcen2424_1975

Alternate gene names: 116689996

Gene position: 2194042-2194917 (Clockwise)

Preceding gene: 116689995

Following gene: 116689997

Centisome position: 62.98

GC content: 69.86

Gene sequence:

>876_bases
ATGGCTGCCACCCTCGACCTCGTCAAACGCCGTCTCGCCGACGCACCGCGCAGCGGTGACACCCGCTCGCCGCATTCCGC
CGCGCGCCGCCGCTCGACCCCGATGCGCCGCCGCGCATGGCGCATCGCATCGCTCGCCGCCTGCATCGCGCTGTGGCAGG
CGGCCGTCCACTGGCGGCTGTCGCTCGGCATCGTCACGTTCGCGAACGTGCCCGCGCCGAGCGACGTCGGCCCCGCGCTG
GCAGCGTTCCTGCAATCGCCGAAGCTGCCGATGCACCTGTTCGCGAGCATCACGCGCGTGCTGGCCGGCTTCGTCGCGGC
GGCGGTCACCGGCATCGGCCTCGGGCTCGCGATCGGCCGCTATCGCGCGCTCGAGGACACGCTGCTGCCGCCGCTCGAGA
TGCTGCGGCCGATTCCGGCCGTCGCGTGGATTCCGCTCGCGATCCTGATGTTCCCGTCGTCCGAGCTGAGCATGATGTTC
ATCACGTTCGTCGGCGCGCTGTTTCCGATCCTGCTCAATACCGTGCATGGCGCCGAAGCCGTCGACCCGCGGCTCGTCGC
GACCGCGCGCAGCCTCGGCACGCGCGGCCTCGCGCTCTATGCGGAAGTGATCCTGCCCGGCGCCGCGCCGTCGATCTTCA
CGGGCCTGTCGATCGGAATGGGTACCGCGTGGTTCTGCCTCGTCACGGCCGAGATGATCGCGGGCCAGTACGGGATCGGC
TATTTCACGTGGGAATCCTATACGTTGCAGAACTACCCGGACATCGTGGTCGGCATGGCGCTGATCGGCCTGCTCGGGAT
GGGCAGCAGCCTGCTCGTCAAGCGTATCGGCGCGGCGCTCACGCCGTGGTACGCACCGCGTGGAGGCCGATCGTGA

Upstream 100 bases:

>100_bases
GTCGACTACGCGGCCGCGAAGACCGTCACCGCGCAGGCGCTCGCCGCCCGCTGAACGTCGCGCGCCTTTCCCCTTCCATT
CGTCGACCCCGGACTTCCGC

Downstream 100 bases:

>100_bases
GTACGCCGGACATTGCGTCGCCCGCGCCCGGCCGCATCGGCGTGCGCGCGCTGACGATCGAACTCGGCCCGCCGCATGCG
CGCTTCGCGGCGCTCGATAC

Product: binding-protein-dependent transport systems inner membrane component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MAATLDLVKRRLADAPRSGDTRSPHSAARRRSTPMRRRAWRIASLAACIALWQAAVHWRLSLGIVTFANVPAPSDVGPAL
AAFLQSPKLPMHLFASITRVLAGFVAAAVTGIGLGLAIGRYRALEDTLLPPLEMLRPIPAVAWIPLAILMFPSSELSMMF
ITFVGALFPILLNTVHGAEAVDPRLVATARSLGTRGLALYAEVILPGAAPSIFTGLSIGMGTAWFCLVTAEMIAGQYGIG
YFTWESYTLQNYPDIVVGMALIGLLGMGSSLLVKRIGAALTPWYAPRGGRS

Sequences:

>Translated_291_residues
MAATLDLVKRRLADAPRSGDTRSPHSAARRRSTPMRRRAWRIASLAACIALWQAAVHWRLSLGIVTFANVPAPSDVGPAL
AAFLQSPKLPMHLFASITRVLAGFVAAAVTGIGLGLAIGRYRALEDTLLPPLEMLRPIPAVAWIPLAILMFPSSELSMMF
ITFVGALFPILLNTVHGAEAVDPRLVATARSLGTRGLALYAEVILPGAAPSIFTGLSIGMGTAWFCLVTAEMIAGQYGIG
YFTWESYTLQNYPDIVVGMALIGLLGMGSSLLVKRIGAALTPWYAPRGGRS
>Mature_290_residues
AATLDLVKRRLADAPRSGDTRSPHSAARRRSTPMRRRAWRIASLAACIALWQAAVHWRLSLGIVTFANVPAPSDVGPALA
AFLQSPKLPMHLFASITRVLAGFVAAAVTGIGLGLAIGRYRALEDTLLPPLEMLRPIPAVAWIPLAILMFPSSELSMMFI
TFVGALFPILLNTVHGAEAVDPRLVATARSLGTRGLALYAEVILPGAAPSIFTGLSIGMGTAWFCLVTAEMIAGQYGIGY
FTWESYTLQNYPDIVVGMALIGLLGMGSSLLVKRIGAALTPWYAPRGGRS

Specific function: Probably part of a high-affinity binding-protein- dependent transport system for nitrate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0600

COG function: function code P; ABC-type nitrate/sulfonate/bicarbonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081802, Length=238, Percent_Identity=36.9747899159664, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786564, Length=225, Percent_Identity=30.6666666666667, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005889 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31037; Mature: 30906

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATLDLVKRRLADAPRSGDTRSPHSAARRRSTPMRRRAWRIASLAACIALWQAAVHWRL
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGIVTFANVPAPSDVGPALAAFLQSPKLPMHLFASITRVLAGFVAAAVTGIGLGLAIGR
HHHEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRALEDTLLPPLEMLRPIPAVAWIPLAILMFPSSELSMMFITFVGALFPILLNTVHGAEA
HHHHHHHCCCCHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
VDPRLVATARSLGTRGLALYAEVILPGAAPSIFTGLSIGMGTAWFCLVTAEMIAGQYGIG
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
YFTWESYTLQNYPDIVVGMALIGLLGMGSSLLVKRIGAALTPWYAPRGGRS
EEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AATLDLVKRRLADAPRSGDTRSPHSAARRRSTPMRRRAWRIASLAACIALWQAAVHWRL
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGIVTFANVPAPSDVGPALAAFLQSPKLPMHLFASITRVLAGFVAAAVTGIGLGLAIGR
HHHEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRALEDTLLPPLEMLRPIPAVAWIPLAILMFPSSELSMMFITFVGALFPILLNTVHGAEA
HHHHHHHCCCCHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
VDPRLVATARSLGTRGLALYAEVILPGAAPSIFTGLSIGMGTAWFCLVTAEMIAGQYGIG
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
YFTWESYTLQNYPDIVVGMALIGLLGMGSSLLVKRIGAALTPWYAPRGGRS
EEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]