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The map label for this gene is mscL [H]

Identifier: 116689972

GI number: 116689972

Start: 2170911

End: 2171342

Strand: Direct

Name: mscL [H]

Synonym: Bcen2424_1951

Alternate gene names: 116689972

Gene position: 2170911-2171342 (Clockwise)

Preceding gene: 116689971

Following gene: 116689973

Centisome position: 62.31

GC content: 58.8

Gene sequence:

>432_bases
ATGAGCATCATCAAGGAATTCAAGGAGTTCGCCGTCAAGGGCAACGTGATGGATCTCGCCGTCGGCGTGATCATCGGCGG
CGCGTTCTCGAAGATCGTCGACTCGGTCGTGAAGGACCTCATCATGCCGGTCATCGGTGTGCTGACCGGCGGTCTGGATT
TCTCGAACAAGTTCGTTCTGCTCGGCACCATCCCCCCGTCGTTCAAGGGTAATCCCGATTCGTTCAAGGACCTGCAGGCC
GCGGGCGTCGCCGCATTCGGCTACGGCTCGTTCATCACGGTGGCGATCAATTTCGTGATCCTTGCGTTCATCATTTTCCT
GATGGTGAAATTCATCAACAAACTGCGCAAGCCGGAAGAAGCCGCGCCGGCCGCGACGCCGGAAGACGTCGTGCTGCTGC
GCGAGATCCGCGATTCGCTCAAGCAGCGCTGA

Upstream 100 bases:

>100_bases
CTATCATGCGGCCATCGGCGTGCCACCGACCTCATTCGACGCAAAGCAATCCTGCGCGTCGCGGCACGCCTCCCTCGCCC
CAACCAGGAGAAAGCCGCTC

Downstream 100 bases:

>100_bases
GCGCCTCACGCGCCGCCCGCCAGGCCCGCCCCGTGCGGGCCTTTTTATTGCGCGCGCGATGCACCGCCGCATGCGGCGTA
TCTCACTTTTTTGTGACGAG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 142

Protein sequence:

>143_residues
MSIIKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVLTGGLDFSNKFVLLGTIPPSFKGNPDSFKDLQA
AGVAAFGYGSFITVAINFVILAFIIFLMVKFINKLRKPEEAAPAATPEDVVLLREIRDSLKQR

Sequences:

>Translated_143_residues
MSIIKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVLTGGLDFSNKFVLLGTIPPSFKGNPDSFKDLQA
AGVAAFGYGSFITVAINFVILAFIIFLMVKFINKLRKPEEAAPAATPEDVVLLREIRDSLKQR
>Mature_142_residues
SIIKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVLTGGLDFSNKFVLLGTIPPSFKGNPDSFKDLQAA
GVAAFGYGSFITVAINFVILAFIIFLMVKFINKLRKPEEAAPAATPEDVVLLREIRDSLKQR

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=146, Percent_Identity=53.4246575342466, Blast_Score=121, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 15449; Mature: 15317

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVLTGGLDFSNKFVL
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LGTIPPSFKGNPDSFKDLQAAGVAAFGYGSFITVAINFVILAFIIFLMVKFINKLRKPEE
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AAPAATPEDVVLLREIRDSLKQR
CCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIIKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVLTGGLDFSNKFVL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LGTIPPSFKGNPDSFKDLQAAGVAAFGYGSFITVAINFVILAFIIFLMVKFINKLRKPEE
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AAPAATPEDVVLLREIRDSLKQR
CCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA