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The map label for this gene is rumA

Identifier: 116689838

GI number: 116689838

Start: 2018880

End: 2020277

Strand: Reverse

Name: rumA

Synonym: Bcen2424_1817

Alternate gene names: 116689838

Gene position: 2020277-2018880 (Counterclockwise)

Preceding gene: 116689840

Following gene: 116689836

Centisome position: 57.99

GC content: 67.67

Gene sequence:

>1398_bases
GTGTCCGAAGCCGTCCCCACTTCTGCGCGCAAATCGAAAAACGCGCCCGTCGCGCCCGGGCCCGCCCCGGTTCTCGAGAT
CGAATCGCTCGACATGGAAGCGCGCGGTGTCGGCCGCACGATGACCGAGGACGGCGAGCCGGGCAAGGTGATCTTCGTCG
AAGGCGCGCTGCCGGGCGAACGCGTGACCTATTCGAGCTATCGCCGCAAGCCGAGCTACGAGCAGGCGACGGTTGTCGAC
ATTCTGCGCCCGAGCGTGATGCGCACCCAGCCGAAGTGCACGTTCTTCGGCACCTGCGGCGGCTGCTCGATGCAGCATCT
CGACATGCGCGCGCAGGTGGCGGTCAAGCAGCGCGTGCTGGAAGATAACCTGTGGCACCTGGCGAAGCTGCGCGCGGAAA
CGATGTTCGCGCCGATCCACGGCCCGTCGTGGGGCTATCGCTACCGGGCGCGCCTGACCGTGCGCAACGTCGCGAAGAAG
GGCGGCGTGCTGGTCGGCTTCCACGAGAAGAAGAGCAGCTACGTGGCCGACATGACGAGCTGCGAAGTGCTGCCGCCGCA
CGTGTCGGCGATGCTCGTGCCGCTGCGGCGGCTCGTCGAGGGGCTGTCGATTCGCGACCGGATGCCGCAGATCGAGCTCG
CGGTCGGCTCGACCGTCACGGCGCTCGTGCTGCGTGTGCTGGAGCCGATCAATGCGGATGACGAAGCGCTGCTGCGCGCG
TTCGCTGACGAGCACAAGGTGCAGTTCTGGCTGCAGCCGAAGGGGCCGGACACGGTGACGCCGTTCTATCCGCTCGACGT
GTCGCTCGACTACACGCTGCCGGAATTCGGCATCCGCATGCCGTTCAAGCCGACTGATTTCACGCAGGTCAATCACCAGA
TCAACCGCGTGCTGGTGGGCCGCGCGCTGCGCCTGCTCGCGCCGTCGCGCGACGATCGCGTGCTCGACCTGTTCTGCGGG
ATCGGCAACTTCACGCTGCCGCTCGCGCGGCTGTCGCGCGAAGTGATGGGCATCGAGGGCAGCGACACGCTGACCACGCG
CGCACTGGCGAACGCACGCGAGAACGGTGTCGACGGCCACACGACGTTCGCGTGCCGGAACCTGTTCGAGGTGACGGGCG
ACGATCTCCGTGCGCTCGGCGCATTCGACAAGTTCCTGATCGACCCGCCGCGCGAAGGCGCGCTCGCGGTGTCGAAGGCG
CTGGCCGAGATCGCGCAGAGCGGCGCAGGCCCGTTGCCGAAGCGCATCGTCTACGTGTCGTGCAACCCGTCGACGCTCGC
GCGCGATGCGGGCCTGCTCGTGCACGAGGCCGGCTACCGGCTGAAGGGCGCCGGGGTCGTGAACATGTTCCCGAATACGT
CGCACGTCGAATCGATCGCGCTGTTCGAGCGCGACTGA

Upstream 100 bases:

>100_bases
GGCCTGCGTATCAGCAGCGGCGCTTGCGCGACGAAAGCACGCGACGGGGGCCTTCGTCGTCTACAATCTCGCCTTCTTCA
ACGTTTGTCAGGAAAAGCTG

Downstream 100 bases:

>100_bases
GCCGGGCGGCGGACGTAAAAAAACCGGCCCGCGGGCCGGTTTTTCATGGTGCGTGCCGAACGTCAGTTGCGGTTGCCGCC
GAAGATGCCGAGCAGCGCGA

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSEAVPTSARKSKNAPVAPGPAPVLEIESLDMEARGVGRTMTEDGEPGKVIFVEGALPGERVTYSSYRRKPSYEQATVVD
ILRPSVMRTQPKCTFFGTCGGCSMQHLDMRAQVAVKQRVLEDNLWHLAKLRAETMFAPIHGPSWGYRYRARLTVRNVAKK
GGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEGLSIRDRMPQIELAVGSTVTALVLRVLEPINADDEALLRA
FADEHKVQFWLQPKGPDTVTPFYPLDVSLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVGRALRLLAPSRDDRVLDLFCG
IGNFTLPLARLSREVMGIEGSDTLTTRALANARENGVDGHTTFACRNLFEVTGDDLRALGAFDKFLIDPPREGALAVSKA
LAEIAQSGAGPLPKRIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPNTSHVESIALFERD

Sequences:

>Translated_465_residues
MSEAVPTSARKSKNAPVAPGPAPVLEIESLDMEARGVGRTMTEDGEPGKVIFVEGALPGERVTYSSYRRKPSYEQATVVD
ILRPSVMRTQPKCTFFGTCGGCSMQHLDMRAQVAVKQRVLEDNLWHLAKLRAETMFAPIHGPSWGYRYRARLTVRNVAKK
GGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEGLSIRDRMPQIELAVGSTVTALVLRVLEPINADDEALLRA
FADEHKVQFWLQPKGPDTVTPFYPLDVSLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVGRALRLLAPSRDDRVLDLFCG
IGNFTLPLARLSREVMGIEGSDTLTTRALANARENGVDGHTTFACRNLFEVTGDDLRALGAFDKFLIDPPREGALAVSKA
LAEIAQSGAGPLPKRIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPNTSHVESIALFERD
>Mature_464_residues
SEAVPTSARKSKNAPVAPGPAPVLEIESLDMEARGVGRTMTEDGEPGKVIFVEGALPGERVTYSSYRRKPSYEQATVVDI
LRPSVMRTQPKCTFFGTCGGCSMQHLDMRAQVAVKQRVLEDNLWHLAKLRAETMFAPIHGPSWGYRYRARLTVRNVAKKG
GVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEGLSIRDRMPQIELAVGSTVTALVLRVLEPINADDEALLRAF
ADEHKVQFWLQPKGPDTVTPFYPLDVSLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVGRALRLLAPSRDDRVLDLFCGI
GNFTLPLARLSREVMGIEGSDTLTTRALANARENGVDGHTTFACRNLFEVTGDDLRALGAFDKFLIDPPREGALAVSKAL
AEIAQSGAGPLPKRIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPNTSHVESIALFERD

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Escherichia coli, GI1789148, Length=445, Percent_Identity=36.1797752808989, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1787083, Length=402, Percent_Identity=24.3781094527363, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790403, Length=355, Percent_Identity=25.0704225352113, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24666579, Length=228, Percent_Identity=30.2631578947368, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_BURCA (Q1BGX9)

Other databases:

- EMBL:   CP000380
- RefSeq:   YP_626099.1
- ProteinModelPortal:   Q1BGX9
- STRING:   Q1BGX9
- GeneID:   4097402
- GenomeReviews:   CP000380_GR
- KEGG:   bcn:Bcen_6262
- eggNOG:   COG2265
- HOGENOM:   HBG690163
- OMA:   VVFVEGA
- ProtClustDB:   PRK13168
- BioCyc:   BCEN331271:BCEN_6262-MONOMER
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 51092; Mature: 50960

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 421-421 BINDING 289-289 BINDING 318-318 BINDING 323-323 BINDING 339-339 BINDING 367-367 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAVPTSARKSKNAPVAPGPAPVLEIESLDMEARGVGRTMTEDGEPGKVIFVEGALPGE
CCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCHHCCCCCCCCCCCCCCEEEEEECCCCCC
RVTYSSYRRKPSYEQATVVDILRPSVMRTQPKCTFFGTCGGCSMQHLDMRAQVAVKQRVL
CEEHHHHCCCCCCCHHHHHHHHCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
EDNLWHLAKLRAETMFAPIHGPSWGYRYRARLTVRNVAKKGGVLVGFHEKKSSYVADMTS
HHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEEHHHHHHHCCEEEEEECCCCCHHHHCCC
CEVLPPHVSAMLVPLRRLVEGLSIRDRMPQIELAVGSTVTALVLRVLEPINADDEALLRA
CCCCCHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHH
FADEHKVQFWLQPKGPDTVTPFYPLDVSLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVG
HHCCCEEEEEECCCCCCCCCCEEEEEEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHH
RALRLLAPSRDDRVLDLFCGIGNFTLPLARLSREVMGIEGSDTLTTRALANARENGVDGH
HHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
TTFACRNLFEVTGDDLRALGAFDKFLIDPPREGALAVSKALAEIAQSGAGPLPKRIVYVS
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEE
CNPSTLARDAGLLVHEAGYRLKGAGVVNMFPNTSHVESIALFERD
CCCHHHHHHCCEEEECCCCEEECCCEEEECCCCCCHHEEEEEECC
>Mature Secondary Structure 
SEAVPTSARKSKNAPVAPGPAPVLEIESLDMEARGVGRTMTEDGEPGKVIFVEGALPGE
CCCCCCCHHHCCCCCCCCCCCCEEEEECCCCHHCCCCCCCCCCCCCCEEEEEECCCCCC
RVTYSSYRRKPSYEQATVVDILRPSVMRTQPKCTFFGTCGGCSMQHLDMRAQVAVKQRVL
CEEHHHHCCCCCCCHHHHHHHHCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
EDNLWHLAKLRAETMFAPIHGPSWGYRYRARLTVRNVAKKGGVLVGFHEKKSSYVADMTS
HHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEEHHHHHHHCCEEEEEECCCCCHHHHCCC
CEVLPPHVSAMLVPLRRLVEGLSIRDRMPQIELAVGSTVTALVLRVLEPINADDEALLRA
CCCCCHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHH
FADEHKVQFWLQPKGPDTVTPFYPLDVSLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVG
HHCCCEEEEEECCCCCCCCCCEEEEEEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHH
RALRLLAPSRDDRVLDLFCGIGNFTLPLARLSREVMGIEGSDTLTTRALANARENGVDGH
HHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
TTFACRNLFEVTGDDLRALGAFDKFLIDPPREGALAVSKALAEIAQSGAGPLPKRIVYVS
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEE
CNPSTLARDAGLLVHEAGYRLKGAGVVNMFPNTSHVESIALFERD
CCCHHHHHHCCEEEECCCCEEECCCEEEECCCCCCHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA