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The map label for this gene is rlmN [H]

Identifier: 116689835

GI number: 116689835

Start: 2016249

End: 2017388

Strand: Reverse

Name: rlmN [H]

Synonym: Bcen2424_1814

Alternate gene names: 116689835

Gene position: 2017388-2016249 (Counterclockwise)

Preceding gene: 116689836

Following gene: 116689834

Centisome position: 57.91

GC content: 64.82

Gene sequence:

>1140_bases
ATGACGAGCGAAACTTCCGTCAATCTTCTCGACTTCGACGCCGAGGGTCTTGTCGCGTACTGCGGCAGCCTGGGCGAGAA
GCCGTTCCGCGCCAAGCAGTTGCAGCGCTGGATCCACCAGTACAACGCCGGCGATTTCGACGGCATGACCGATCTCGCGA
AGTCCCTGAGAGAAAAGCTCAAGGGCCGCGCGTCGATCGTGATGCCGGACATCGCCAGCGATCACGTTTCCACCGACGGC
ACGCGCAAGTGGCTGATCGACGTCGGAAACGGCAATGCGGTCGAAACCGTGTTCATCCCGGAAGAGACGCGCGGCACGCT
GTGCGTGTCGTCGCAGGCCGGGTGCGCGGTGAACTGCCGCTTCTGCTCGACGGGCAAGCAGGGCTTCTCCCGCAACCTGT
CGACAGCCGAAATCATCGGCCAGCTCCGGATGGCCGAATTTGCACTGCGAGCGTCGCTGGGCCGCGCGCCCGGCCCGAAC
GGCAAGGCCGAACGGGTCGTCACCAACGTGGTGATGATGGGCATGGGCGAGCCGCTCCTGAATTACAGCGCGGTCGTCCC
CGCGATGCGGCTGATGCTCGACGACAACGCGTACGGCCTGTCGCGCCGTCGCGTCACGCTGTCGACGTCCGGCGTGGTGC
CGATGATGGACCGCCTCGGCGCCGAGTTGCCGGTCGCGCTGGCCGTTTCGCTGCATGCGCCGAACGACGCGCTGCGCGAC
GAGCTGGTGCCGCTCAACAAGAAGTATCCGCTTCGCGAGCTGATGGCTGCGTGCCAGCGTTATCTGAAGGTCGCGCCGCG
CGACTTCATCACTTTCGAATACTGCATGCTCGACGGCGTCAACGACACCGAAGCGCATGCGCGCGAACTGCTGGCCGTCA
CGCGTGACGTGCCGTGCAAGTTCAACCTGATCCCGTTCAATCCGTTTCCGGAATCGGGCCTCATCCGCTCGAAGCCGGAG
CAGATCAAGCGCTTCGCGCAAGTCCTCATCGATGCGGGTGTCGTCACGACCGTGCGCAAGACGCGCGGCGACGACATCGA
CGCCGCGTGCGGGCAGTTGGCCGGCGCGGTGAAGGATCGCACGCGCCTCGCGGAACGAACGGGCGCAGCAGGAAAAATCA
TCGAAGTTCGGGCAGTTTGA

Upstream 100 bases:

>100_bases
GAAATCGCGTTCTTCTTCCCGGAAATGAACGTTTACTCGCGTTAAGCCCTGGCTGACAGCAAGTAGGCAGGATTCAGCGC
ACGCGGCAAGGCAGCACATC

Downstream 100 bases:

>100_bases
CGGGGGCACCTGGCCGGGGTGCCCGAACCCTTTGGCGGTGCAACACGCGAATAGCGATCGGTCGCGGCGCCTCGGCGCCG
CGCATGGTATGAACCGTTGC

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MTSETSVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAGDFDGMTDLAKSLREKLKGRASIVMPDIASDHVSTDG
TRKWLIDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTAEIIGQLRMAEFALRASLGRAPGPN
GKAERVVTNVVMMGMGEPLLNYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
ELVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCKFNLIPFNPFPESGLIRSKPE
QIKRFAQVLIDAGVVTTVRKTRGDDIDAACGQLAGAVKDRTRLAERTGAAGKIIEVRAV

Sequences:

>Translated_379_residues
MTSETSVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAGDFDGMTDLAKSLREKLKGRASIVMPDIASDHVSTDG
TRKWLIDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTAEIIGQLRMAEFALRASLGRAPGPN
GKAERVVTNVVMMGMGEPLLNYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
ELVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCKFNLIPFNPFPESGLIRSKPE
QIKRFAQVLIDAGVVTTVRKTRGDDIDAACGQLAGAVKDRTRLAERTGAAGKIIEVRAV
>Mature_378_residues
TSETSVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAGDFDGMTDLAKSLREKLKGRASIVMPDIASDHVSTDGT
RKWLIDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTAEIIGQLRMAEFALRASLGRAPGPNG
KAERVVTNVVMMGMGEPLLNYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRDE
LVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCKFNLIPFNPFPESGLIRSKPEQ
IKRFAQVLIDAGVVTTVRKTRGDDIDAACGQLAGAVKDRTRLAERTGAAGKIIEVRAV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=380, Percent_Identity=53.9473684210526, Blast_Score=403, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004383 [H]

Pfam domain/function: NA

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41338; Mature: 41207

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSETSVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAGDFDGMTDLAKSLREKL
CCCCCCEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGRASIVMPDIASDHVSTDGTRKWLIDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCR
CCCCEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEE
FCSTGKQGFSRNLSTAEIIGQLRMAEFALRASLGRAPGPNGKAERVVTNVVMMGMGEPLL
EECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHH
NYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEECCCHHHHH
ELVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCK
HCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEEEHEECCCCCHHHHHHHHHHHHCCCCCE
FNLIPFNPFPESGLIRSKPEQIKRFAQVLIDAGVVTTVRKTRGDDIDAACGQLAGAVKDR
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
TRLAERTGAAGKIIEVRAV
HHHHHHCCCCCCEEEEEEC
>Mature Secondary Structure 
TSETSVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAGDFDGMTDLAKSLREKL
CCCCCEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGRASIVMPDIASDHVSTDGTRKWLIDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCR
CCCCEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEE
FCSTGKQGFSRNLSTAEIIGQLRMAEFALRASLGRAPGPNGKAERVVTNVVMMGMGEPLL
EECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHH
NYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEECCCHHHHH
ELVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCK
HCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEEEHEECCCCCHHHHHHHHHHHHCCCCCE
FNLIPFNPFPESGLIRSKPEQIKRFAQVLIDAGVVTTVRKTRGDDIDAACGQLAGAVKDR
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
TRLAERTGAAGKIIEVRAV
HHHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA