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The map label for this gene is ghrB [H]

Identifier: 116689752

GI number: 116689752

Start: 1922348

End: 1923313

Strand: Reverse

Name: ghrB [H]

Synonym: Bcen2424_1731

Alternate gene names: 116689752

Gene position: 1923313-1922348 (Counterclockwise)

Preceding gene: 116689753

Following gene: 116689751

Centisome position: 55.21

GC content: 70.7

Gene sequence:

>966_bases
ATGAAGCCCCGTATCGTCGCGTACAAGCCGCTGCCCGATGACGTTCTCGCGTACCTGCGCGAGCATGCGGACGTCGTGCA
GGCCGACGGCGCCGACGCGCTCGCCGCCGCGCTCGGCGACGCGGACGGCGCGATCGGCGCGAGCCTGAAGGTCACGCCGC
AGATGCTCGATCGTGCGCCGCGGCTGAAGGCGTGGTCGACGATCTCGGTCGGCTACGACAACTTCGACGTCGCCGATTTC
ACGCGGCGCGGCATCGTGCTCGCGCATACGCCGGACGTGCTGACCGAGTCGACGGCCGATACGGTGTTTTCGCTGATCCT
GGCCTCCGCGCGGCGCGTGGTCGAACTGGCCGAATGGGTGAAGGCCGGCCACTGGCATCGCAGCATCGGCCCGGACCTGT
ACGGCACCGACGTGCAGGGCAAGACGCTTGGCATCGTCGGGCTCGGGCGGATCGGCGGCGCGGTCGCGCGCCGCGCGGCG
CTCGGCTTCCGGATGCAGGTGCTGTACGCGAACCGGTCCGCGCATGCCGAGGCCGAGACGCAGTACGGCGCCCGGCGCGT
GACGCTCGACGAGTTGCTCGCGCAGTCCGATTTCGTGTGCCTGCAGGTGCCGCTGTCGCCCGACACGCGCCACCTGATCG
GCGCGCCCGAATTCGCGAAGATGAAACGCGGCGCGATCCTGATCAATGCATCGCGCGGGCCCGTGGTCGACGAGGCCGCG
CTGGTCGACGCACTGCGCGCGGGGACGATCCGCGGCGCGGGGCTCGACGTGTTCGAGAAGGAGCCGCTGCCGGCGGATTC
GCCGCTGCTGCAGATGAAGAACGTCGTCGCGCTGCCGCATATCGGCTCGGCGACGCACGAGACGCGCCATGCGATGGCGC
GCTGCGCCGCGGAAAACCTCGTCGGCGCGCTGGCCGGCACGCTGCGCACGAATCTCGTCAATCCGGATGCGCTGGCGCAC
GCCTGA

Upstream 100 bases:

>100_bases
AGCGCCGCGCTCGTCGCCGCGGTGATCCTCACGCTGTCCGTCAAACCCCAGGCGCGCGATGCGCATCCGCTCGCGGCGCC
GCTGCAAGGAAAATGAAACG

Downstream 100 bases:

>100_bases
GCCGCCCGAGGCAACCGGCCGGCACGGTGCCGGCGCCGTTAGAGTCGATGATCTTGGTGCCGGAGGGCTGGCTGCGTTAT
GATCGCCCGCGCTCGTCGTA

Product: gluconate 2-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKPRIVAYKPLPDDVLAYLREHADVVQADGADALAAALGDADGAIGASLKVTPQMLDRAPRLKAWSTISVGYDNFDVADF
TRRGIVLAHTPDVLTESTADTVFSLILASARRVVELAEWVKAGHWHRSIGPDLYGTDVQGKTLGIVGLGRIGGAVARRAA
LGFRMQVLYANRSAHAEAETQYGARRVTLDELLAQSDFVCLQVPLSPDTRHLIGAPEFAKMKRGAILINASRGPVVDEAA
LVDALRAGTIRGAGLDVFEKEPLPADSPLLQMKNVVALPHIGSATHETRHAMARCAAENLVGALAGTLRTNLVNPDALAH
A

Sequences:

>Translated_321_residues
MKPRIVAYKPLPDDVLAYLREHADVVQADGADALAAALGDADGAIGASLKVTPQMLDRAPRLKAWSTISVGYDNFDVADF
TRRGIVLAHTPDVLTESTADTVFSLILASARRVVELAEWVKAGHWHRSIGPDLYGTDVQGKTLGIVGLGRIGGAVARRAA
LGFRMQVLYANRSAHAEAETQYGARRVTLDELLAQSDFVCLQVPLSPDTRHLIGAPEFAKMKRGAILINASRGPVVDEAA
LVDALRAGTIRGAGLDVFEKEPLPADSPLLQMKNVVALPHIGSATHETRHAMARCAAENLVGALAGTLRTNLVNPDALAH
A
>Mature_321_residues
MKPRIVAYKPLPDDVLAYLREHADVVQADGADALAAALGDADGAIGASLKVTPQMLDRAPRLKAWSTISVGYDNFDVADF
TRRGIVLAHTPDVLTESTADTVFSLILASARRVVELAEWVKAGHWHRSIGPDLYGTDVQGKTLGIVGLGRIGGAVARRAA
LGFRMQVLYANRSAHAEAETQYGARRVTLDELLAQSDFVCLQVPLSPDTRHLIGAPEFAKMKRGAILINASRGPVVDEAA
LVDALRAGTIRGAGLDVFEKEPLPADSPLLQMKNVVALPHIGSATHETRHAMARCAAENLVGALAGTLRTNLVNPDALAH
A

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=257, Percent_Identity=45.136186770428, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI23308577, Length=294, Percent_Identity=35.7142857142857, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI145580578, Length=285, Percent_Identity=35.0877192982456, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4557499, Length=285, Percent_Identity=35.0877192982456, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4557497, Length=296, Percent_Identity=35.472972972973, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI61743967, Length=296, Percent_Identity=35.472972972973, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI145580575, Length=282, Percent_Identity=35.1063829787234, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI87082289, Length=320, Percent_Identity=58.4375, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1789279, Length=258, Percent_Identity=35.2713178294574, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787645, Length=191, Percent_Identity=29.8429319371728, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081824, Length=192, Percent_Identity=31.25, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17532191, Length=301, Percent_Identity=33.5548172757475, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI25147481, Length=283, Percent_Identity=29.3286219081272, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6324055, Length=233, Percent_Identity=42.0600858369099, Blast_Score=173, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6320925, Length=262, Percent_Identity=35.4961832061069, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6322116, Length=262, Percent_Identity=34.7328244274809, Blast_Score=136, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6324964, Length=249, Percent_Identity=29.718875502008, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321253, Length=180, Percent_Identity=31.1111111111111, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6325144, Length=178, Percent_Identity=31.4606741573034, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324980, Length=179, Percent_Identity=32.4022346368715, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571528, Length=278, Percent_Identity=43.5251798561151, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI45552429, Length=310, Percent_Identity=39.3548387096774, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI45551003, Length=310, Percent_Identity=39.3548387096774, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI28574284, Length=310, Percent_Identity=39.3548387096774, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24585514, Length=310, Percent_Identity=39.3548387096774, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI28574282, Length=310, Percent_Identity=39.3548387096774, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI28574286, Length=311, Percent_Identity=35.6913183279743, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24585516, Length=253, Percent_Identity=35.1778656126482, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI19921140, Length=300, Percent_Identity=35.3333333333333, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24646446, Length=294, Percent_Identity=36.3945578231293, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24646448, Length=294, Percent_Identity=36.3945578231293, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24646452, Length=294, Percent_Identity=36.3945578231293, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24646450, Length=294, Percent_Identity=36.3945578231293, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI62472511, Length=294, Percent_Identity=36.3945578231293, Blast_Score=123, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34149; Mature: 34149

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPRIVAYKPLPDDVLAYLREHADVVQADGADALAAALGDADGAIGASLKVTPQMLDRAP
CCCCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEECHHHHCCCC
RLKAWSTISVGYDNFDVADFTRRGIVLAHTPDVLTESTADTVFSLILASARRVVELAEWV
CCCCCEEEEECCCCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAGHWHRSIGPDLYGTDVQGKTLGIVGLGRIGGAVARRAALGFRMQVLYANRSAHAEAET
HHCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCEEEEEEEECCCCCCHHHH
QYGARRVTLDELLAQSDFVCLQVPLSPDTRHLIGAPEFAKMKRGAILINASRGPVVDEAA
HHCCEEEEHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHCCCEEEEECCCCCCCHHHH
LVDALRAGTIRGAGLDVFEKEPLPADSPLLQMKNVVALPHIGSATHETRHAMARCAAENL
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCEEECCCCCCCHHHHHHHHHHHHHHHH
VGALAGTLRTNLVNPDALAHA
HHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MKPRIVAYKPLPDDVLAYLREHADVVQADGADALAAALGDADGAIGASLKVTPQMLDRAP
CCCCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEECHHHHCCCC
RLKAWSTISVGYDNFDVADFTRRGIVLAHTPDVLTESTADTVFSLILASARRVVELAEWV
CCCCCEEEEECCCCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAGHWHRSIGPDLYGTDVQGKTLGIVGLGRIGGAVARRAALGFRMQVLYANRSAHAEAET
HHCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCEEEEEEEECCCCCCHHHH
QYGARRVTLDELLAQSDFVCLQVPLSPDTRHLIGAPEFAKMKRGAILINASRGPVVDEAA
HHCCEEEEHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHCCCEEEEECCCCCCCHHHH
LVDALRAGTIRGAGLDVFEKEPLPADSPLLQMKNVVALPHIGSATHETRHAMARCAAENL
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCEEECCCCCCCHHHHHHHHHHHHHHHH
VGALAGTLRTNLVNPDALAHA
HHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA