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The map label for this gene is hipO [H]

Identifier: 116689671

GI number: 116689671

Start: 1831983

End: 1833146

Strand: Direct

Name: hipO [H]

Synonym: Bcen2424_1649

Alternate gene names: 116689671

Gene position: 1831983-1833146 (Clockwise)

Preceding gene: 116689668

Following gene: 116689672

Centisome position: 52.58

GC content: 66.92

Gene sequence:

>1164_bases
GTGACGTACAGCGTGATTCCCGCCGGCCTCGCCGACGAAATGATCGAGATCCGGCACCGCATCCATGCCCACCCCGAGCT
GGGTTTCGAGGAATTCGCGACGAGCGACCTCGTCGCCGAGCAGTTGCAGTCGTGGGGCTACACCGTGCATCGCGGGCTCG
GCGGCACCGGCGTCGTCGCCCAGTTGAAAGTGGGCGACGGCAAGCGGCGCCTCGGTCTGCGCGCCGACATGGACGCGCTG
CCGATCCACGAAACGACGGGCCTGCCGTACCAGAGCACGATCGCGGGCAAGATGCACGCGTGCGGCCACGACGGCCACAC
GGCGATGCTGCTCGCAGCCGCGAAGCACCTCGCGCGCGAACGCCGCTTCTCGGGCACGCTGAACCTGATCTTCCAGCCGG
CCGAGGAAGGGCTCGGCGGCGCCAAGAAGATGCTCGACGAAGGGCTGTTCGAGCAATTTCCGTGTGACGCGATCTTCGCG
ATGCACAACATGCCGGGCTTCCCGACCGGCAAATTCGGTTTCCTGCCGGGCTCGTTCATGGCGTCGTCGGATACCGTCGT
CATCGACGTGCAGGGTCGCGGCGGCCACGGCGCGGTGCCGCACCGGGCGATCGATCCGGTCGTCGTATGCGCGCAGATCG
TGCTGGCGCTGCAGACCATCGTGTCGCGCAACGTGTCGCCGCTCGACATGGCGATCATCACGGTCGGTGCGATCCATGCG
GGCGAAGCGCCGAACGTGATTCCCGATCGCGCGCAGATGCGCCTGTCGGTGCGCGCGCTGAAGCCCGACGTGCGCGACTT
GCTCGAAACGCGCATCAAGGAGGTCGTCCATACGCAGGCGGCCGTGTTCGGCGCGACCGCGACGATCGACTACCAGCGCC
GCTACCCAGTGCTCGTGAACGATGCGGAGATGACGGCGTTCGCGCGCGGCGTGGCCCGTGAATGGGTCGGCGAAGCCAAT
CTGATCGACGGCATGGTGCCGCTCACCGGCAGCGAGGATTTCGCGTTCCTGCTCGAGAAACGGCCGGGCTGCTACCTGAT
CATCGGCAACGGCGACGGGGAGGGCGGCTGCATGGTGCACAACCCCGGCTACGACTTCAACGACGCGGCGCTGCCGACCG
GCGCGTCGTATTGGGTGAAGTTAACTGAGGCGTTTCTGGTGTGA

Upstream 100 bases:

>100_bases
GGCAATCGCCAGTTGCCATCGTGACGACTTTCTGTCTATCCGGTTGCCGTTCGTCGGACTATCCTTGCCCGCATACTCAC
AGCACACAGGAGACGACACC

Downstream 100 bases:

>100_bases
CGGAGGATGCGGCGTGAGGTTGGAGCCGGGAATTCACAATCCGCTCCCAACCATGACAGACGGCATGTACCTTGCCTTGG
TATTTGGTATATGCCCATCG

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MTYSVIPAGLADEMIEIRHRIHAHPELGFEEFATSDLVAEQLQSWGYTVHRGLGGTGVVAQLKVGDGKRRLGLRADMDAL
PIHETTGLPYQSTIAGKMHACGHDGHTAMLLAAAKHLARERRFSGTLNLIFQPAEEGLGGAKKMLDEGLFEQFPCDAIFA
MHNMPGFPTGKFGFLPGSFMASSDTVVIDVQGRGGHGAVPHRAIDPVVVCAQIVLALQTIVSRNVSPLDMAIITVGAIHA
GEAPNVIPDRAQMRLSVRALKPDVRDLLETRIKEVVHTQAAVFGATATIDYQRRYPVLVNDAEMTAFARGVAREWVGEAN
LIDGMVPLTGSEDFAFLLEKRPGCYLIIGNGDGEGGCMVHNPGYDFNDAALPTGASYWVKLTEAFLV

Sequences:

>Translated_387_residues
MTYSVIPAGLADEMIEIRHRIHAHPELGFEEFATSDLVAEQLQSWGYTVHRGLGGTGVVAQLKVGDGKRRLGLRADMDAL
PIHETTGLPYQSTIAGKMHACGHDGHTAMLLAAAKHLARERRFSGTLNLIFQPAEEGLGGAKKMLDEGLFEQFPCDAIFA
MHNMPGFPTGKFGFLPGSFMASSDTVVIDVQGRGGHGAVPHRAIDPVVVCAQIVLALQTIVSRNVSPLDMAIITVGAIHA
GEAPNVIPDRAQMRLSVRALKPDVRDLLETRIKEVVHTQAAVFGATATIDYQRRYPVLVNDAEMTAFARGVAREWVGEAN
LIDGMVPLTGSEDFAFLLEKRPGCYLIIGNGDGEGGCMVHNPGYDFNDAALPTGASYWVKLTEAFLV
>Mature_386_residues
TYSVIPAGLADEMIEIRHRIHAHPELGFEEFATSDLVAEQLQSWGYTVHRGLGGTGVVAQLKVGDGKRRLGLRADMDALP
IHETTGLPYQSTIAGKMHACGHDGHTAMLLAAAKHLARERRFSGTLNLIFQPAEEGLGGAKKMLDEGLFEQFPCDAIFAM
HNMPGFPTGKFGFLPGSFMASSDTVVIDVQGRGGHGAVPHRAIDPVVVCAQIVLALQTIVSRNVSPLDMAIITVGAIHAG
EAPNVIPDRAQMRLSVRALKPDVRDLLETRIKEVVHTQAAVFGATATIDYQRRYPVLVNDAEMTAFARGVAREWVGEANL
IDGMVPLTGSEDFAFLLEKRPGCYLIIGNGDGEGGCMVHNPGYDFNDAALPTGASYWVKLTEAFLV

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=415, Percent_Identity=27.2289156626506, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41708; Mature: 41577

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYSVIPAGLADEMIEIRHRIHAHPELGFEEFATSDLVAEQLQSWGYTVHRGLGGTGVVA
CCCEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEE
QLKVGDGKRRLGLRADMDALPIHETTGLPYQSTIAGKMHACGHDGHTAMLLAAAKHLARE
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHCCCCCCCHHHHHHHHHHHHHH
RRFSGTLNLIFQPAEEGLGGAKKMLDEGLFEQFPCDAIFAMHNMPGFPTGKFGFLPGSFM
HHCCCEEEEEEECCHHCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCC
ASSDTVVIDVQGRGGHGAVPHRAIDPVVVCAQIVLALQTIVSRNVSPLDMAIITVGAIHA
CCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEEEEC
GEAPNVIPDRAQMRLSVRALKPDVRDLLETRIKEVVHTQAAVFGATATIDYQRRYPVLVN
CCCCCCCCCCHHHEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEE
DAEMTAFARGVAREWVGEANLIDGMVPLTGSEDFAFLLEKRPGCYLIIGNGDGEGGCMVH
CHHHHHHHHHHHHHHCCCCHHHCCEECCCCCCCCEEEEECCCCEEEEEECCCCCCCEEEE
NPGYDFNDAALPTGASYWVKLTEAFLV
CCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TYSVIPAGLADEMIEIRHRIHAHPELGFEEFATSDLVAEQLQSWGYTVHRGLGGTGVVA
CCEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEE
QLKVGDGKRRLGLRADMDALPIHETTGLPYQSTIAGKMHACGHDGHTAMLLAAAKHLARE
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHCCCCCCCHHHHHHHHHHHHHH
RRFSGTLNLIFQPAEEGLGGAKKMLDEGLFEQFPCDAIFAMHNMPGFPTGKFGFLPGSFM
HHCCCEEEEEEECCHHCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCC
ASSDTVVIDVQGRGGHGAVPHRAIDPVVVCAQIVLALQTIVSRNVSPLDMAIITVGAIHA
CCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEEEEC
GEAPNVIPDRAQMRLSVRALKPDVRDLLETRIKEVVHTQAAVFGATATIDYQRRYPVLVN
CCCCCCCCCCHHHEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEE
DAEMTAFARGVAREWVGEANLIDGMVPLTGSEDFAFLLEKRPGCYLIIGNGDGEGGCMVH
CHHHHHHHHHHHHHHCCCCHHHCCEECCCCCCCCEEEEECCCCEEEEEECCCCCCCEEEE
NPGYDFNDAALPTGASYWVKLTEAFLV
CCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]