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The map label for this gene is mexA [H]

Identifier: 116689644

GI number: 116689644

Start: 1786759

End: 1787961

Strand: Direct

Name: mexA [H]

Synonym: Bcen2424_1622

Alternate gene names: 116689644

Gene position: 1786759-1787961 (Clockwise)

Preceding gene: 116689641

Following gene: 116689645

Centisome position: 51.29

GC content: 72.82

Gene sequence:

>1203_bases
ATGAATAACAAACGCACCCAGTGGCGCCGCATGCGGCTGGCGCCGTTCGCGCTCGCGGCCCTGTTGGCCGTGGCCGGATG
CGGAAAGGGCGACAAGGATGCCGCGCCGGAGACCGCGAAGCAGGCCACCGTCGTGACGGTGCGCCCGACGGCCGTGCCGA
TGACTGTCGAACTGCCGGGCCGGCTCGACGCCTACCGGCAGGCGGAAGTCCGCGCGCGGGTCGCGGGCATCGTCATCGCA
CGCACCTACGAGGAAGGCCAGGAAGTGAAGCAGGGCGCGGTGCTGTTCCGCATCGATCCTGCGCCGCTGAAGGCCGCACG
CGACGCCGCGCAGGGCGCGCTCGCGAAGGCGCAGGCCGCCGCGCTCGCGGCGACCGACAAGCGCCGCCGTTACGACGATC
TCGTGCGCGACCGCGCGGTGAGCGAGCGCGACCATACCGAAGCCGTCGCGGCCGATACGCAGGCGAAGGCCGAGGTGGCA
TCCGCGAAGGCCGAACTCGCGCGCGCGCAGTTGCAGCTCGATTACGCGACCGTCACCGCGCCGATCGCGGGCCGCGCACG
GCGCGCGCTCGTGACCGAGGGCGCGCTCGTCGGCCAGGACCAGGCGACGCCGCTCACGACCGTCGAGCAGCTCGATCCGA
TCTACGTGAACTTCTCGCAGCCGGCCGCCGACGTCGACGCCTTGCGTCGCGCGGTGAAGAGCGGGCGCGCGACGGGCATC
GCGCAGCACGACGTCGCGGTGACGCTGCTGCGCGCCGACGGCACCGCGTATCCGCTGAAGGGCAAGCTGCTGTTCAGCGA
TCTCGCGGTCGATCCGACCACCGACACCGTCGCGATGCGCGCGCTGTTCCCCAATCCGGAGCGCGAGCTGCTGCCGGGCG
CGTACGTGCGGATCGCGCTCGACACGGCGGTCGACCAGCGGGCGATCCTGGTGCCGCGCGACGCGCTGCTGCGCACCGCC
GACCGGACGTCGGTGCGGGTCGTCGGCGCGAACGGCAAGGTCAAGGACGTCGAGGTCACGGCCGACCAGATGAGCGGCCG
TGACTGGCGCATCACGCGCGGCCTCGCGGGCGGCGAGCGCGTGATCGTCGACGACGCCGCGCAGTTCGCGCCCGATACGG
CCGTCAAGCCCGTCGAAAAAGCGCCGCCGTCGAAGGCGGCGCCGGCAGCGGCCGCTTCGCAGGCGGCCGCCCGTCAAACC
TGA

Upstream 100 bases:

>100_bases
CCAGATTGAAAGCGCGCGTATTTAATCAGTCGTGACTGATTATAATCGGCCACCCTGAGCCGCCGCATTGTATCGGCGGC
GAATGATCCGAGGTCAGAAC

Downstream 100 bases:

>100_bases
CCGGTTCAAACCAACATGGCACGTTTCTTCATCGATCGCCCCGTCTTCGCGTGGGTGATCGCCATCTTCATCATGCTGGG
CGGCCTGTTCGCGATCCGCG

Product: periplasmic multidrug efflux lipoprotein precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MNNKRTQWRRMRLAPFALAALLAVAGCGKGDKDAAPETAKQATVVTVRPTAVPMTVELPGRLDAYRQAEVRARVAGIVIA
RTYEEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAAALAATDKRRRYDDLVRDRAVSERDHTEAVAADTQAKAEVA
SAKAELARAQLQLDYATVTAPIAGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVDALRRAVKSGRATGI
AQHDVAVTLLRADGTAYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIALDTAVDQRAILVPRDALLRTA
DRTSVRVVGANGKVKDVEVTADQMSGRDWRITRGLAGGERVIVDDAAQFAPDTAVKPVEKAPPSKAAPAAAASQAAARQT

Sequences:

>Translated_400_residues
MNNKRTQWRRMRLAPFALAALLAVAGCGKGDKDAAPETAKQATVVTVRPTAVPMTVELPGRLDAYRQAEVRARVAGIVIA
RTYEEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAAALAATDKRRRYDDLVRDRAVSERDHTEAVAADTQAKAEVA
SAKAELARAQLQLDYATVTAPIAGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVDALRRAVKSGRATGI
AQHDVAVTLLRADGTAYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIALDTAVDQRAILVPRDALLRTA
DRTSVRVVGANGKVKDVEVTADQMSGRDWRITRGLAGGERVIVDDAAQFAPDTAVKPVEKAPPSKAAPAAAASQAAARQT
>Mature_400_residues
MNNKRTQWRRMRLAPFALAALLAVAGCGKGDKDAAPETAKQATVVTVRPTAVPMTVELPGRLDAYRQAEVRARVAGIVIA
RTYEEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAAALAATDKRRRYDDLVRDRAVSERDHTEAVAADTQAKAEVA
SAKAELARAQLQLDYATVTAPIAGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVDALRRAVKSGRATGI
AQHDVAVTLLRADGTAYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIALDTAVDQRAILVPRDALLRTA
DRTSVRVVGANGKVKDVEVTADQMSGRDWRITRGLAGGERVIVDDAAQFAPDTAVKPVEKAPPSKAAPAAAASQAAARQT

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=387, Percent_Identity=40.3100775193798, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1786668, Length=368, Percent_Identity=41.0326086956522, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1789929, Length=364, Percent_Identity=38.1868131868132, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI87082036, Length=333, Percent_Identity=31.5315315315315, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42483; Mature: 42483

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKRTQWRRMRLAPFALAALLAVAGCGKGDKDAAPETAKQATVVTVRPTAVPMTVELPG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCC
RLDAYRQAEVRARVAGIVIARTYEEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAA
CCCHHHHHHHHHHHEEEEEEEEHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
ALAATDKRRRYDDLVRDRAVSERDHTEAVAADTQAKAEVASAKAELARAQLQLDYATVTA
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEHEEEC
PIAGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVDALRRAVKSGRATGI
CCCCHHHHHHHCCCCEECCCCCCCCCHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCE
AQHDVAVTLLRADGTAYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIAL
EECCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHCCCEEEEEEE
DTAVDQRAILVPRDALLRTADRTSVRVVGANGKVKDVEVTADQMSGRDWRITRGLAGGER
ECCCCCCEEECCHHHHHHCCCCCEEEEECCCCCEEEEEEEHHHHCCCCEEEEECCCCCCE
VIVDDAAQFAPDTAVKPVEKAPPSKAAPAAAASQAAARQT
EEEECHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNNKRTQWRRMRLAPFALAALLAVAGCGKGDKDAAPETAKQATVVTVRPTAVPMTVELPG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCC
RLDAYRQAEVRARVAGIVIARTYEEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAA
CCCHHHHHHHHHHHEEEEEEEEHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
ALAATDKRRRYDDLVRDRAVSERDHTEAVAADTQAKAEVASAKAELARAQLQLDYATVTA
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEHEEEC
PIAGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVDALRRAVKSGRATGI
CCCCHHHHHHHCCCCEECCCCCCCCCHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCE
AQHDVAVTLLRADGTAYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIAL
EECCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHCCCEEEEEEE
DTAVDQRAILVPRDALLRTADRTSVRVVGANGKVKDVEVTADQMSGRDWRITRGLAGGER
ECCCCCCEEECCHHHHHHCCCCCEEEEECCCCCEEEEEEEHHHHCCCCEEEEECCCCCCE
VIVDDAAQFAPDTAVKPVEKAPPSKAAPAAAASQAAARQT
EEEECHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]