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The map label for this gene is cysU [H]

Identifier: 116689624

GI number: 116689624

Start: 1761856

End: 1762752

Strand: Direct

Name: cysU [H]

Synonym: Bcen2424_1602

Alternate gene names: 116689624

Gene position: 1761856-1762752 (Clockwise)

Preceding gene: 116689623

Following gene: 116689625

Centisome position: 50.57

GC content: 67.89

Gene sequence:

>897_bases
ATGACGACGTACACCTTTCGCAAGCCGAGCGCGCTGCCCGGTTTCGGCGTGACACTCGGCATCACGGTGGCCTATTTGAG
CCTCGTGGTGCTGATTCCGCTCGCCGCCACGTTCCTGAAGACCGCGACGCTGTCGTGGGACCAGTTCGTCACCGCCGTCA
CGTCGCCGCGCGTGCTCGCGTCGTACCGGCTGACGTTCTCGGCCGCGCTCGGCGGCGCGCTGATCAATGCCGTGTTCGGC
TTCCTCGTCGCATGGGTGCTGGTGCGCTACACGTTCCCGTTCAAGCGCCTCGTCGATGCGGTCGTCGACCTGCCGTTCGC
GCTGCCGACGTCGGTGGCCGGCATCTCGCTCGCGGCCGTCTACGCGACCAACGGCTGGGTCGGCCAGTATCTGGCGCCGC
TCGGCATCAAGATCGCGTTCACGCCGGCCGGCGTGCTGGTCGCGCTGACCTTCATCGGGCTGCCGTTCGTCGTGCGCACC
GTGCAGCCGGTGCTCGAGGATTTCGAGCGCGAGCAGGAAGAAGCCGCCGCGTGCCTCGGCGCGTCGCGCTGGCTGACGTT
CCGCCGCGTCGTGTTGCCGGCCGTGCTGCCGGCGCTGCTCACCGGTTTCGCGCTCGCGTTCGCGCGCGCGCTCGGCGAAT
ACGGCTCGGTGATCTTCATCGCCGGCAACGTGCCGATGAAATCCGAGATCACGTCGCTATTGATCATCACGAAGCTCGAG
CAGTACGACTACGCGGGCGCGACCGCGCTGGCGGTCGTGATGCTGGTCGTGTCGTTCCTGATGCTGCTGCTGATCAACAC
GCTGCAGTGGTATCTGCAGCGTCGTACGAGCAAGGGCGCGAGCGGTCCGGCGCCCGCCACCGTCACCGCCGCTGCTGCCG
CCGCAGGAGGCCAGTAA

Upstream 100 bases:

>100_bases
CACGACAGCGCGCCGCACCCCCGACGGGCCGCCGGCGCGATTCACTGCGCCGGCGTTTGCGTCGGACCCGTGAGCAGCAT
CGACCCAGCAAGAGCATCCG

Downstream 100 bases:

>100_bases
TGACCCAGGAGGCCACCGTCGTGCTGAAAACCCCGTCGCCGGCCGCGCGTGCCGCGAAGCGGCTCGACCCCGTCAGCGAG
TCGCGCGTCGTGCGCTGGCT

Product: sulfate ABC transporter, inner membrane subunit CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTTYTFRKPSALPGFGVTLGITVAYLSLVVLIPLAATFLKTATLSWDQFVTAVTSPRVLASYRLTFSAALGGALINAVFG
FLVAWVLVRYTFPFKRLVDAVVDLPFALPTSVAGISLAAVYATNGWVGQYLAPLGIKIAFTPAGVLVALTFIGLPFVVRT
VQPVLEDFEREQEEAAACLGASRWLTFRRVVLPAVLPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
QYDYAGATALAVVMLVVSFLMLLLINTLQWYLQRRTSKGASGPAPATVTAAAAAAGGQ

Sequences:

>Translated_298_residues
MTTYTFRKPSALPGFGVTLGITVAYLSLVVLIPLAATFLKTATLSWDQFVTAVTSPRVLASYRLTFSAALGGALINAVFG
FLVAWVLVRYTFPFKRLVDAVVDLPFALPTSVAGISLAAVYATNGWVGQYLAPLGIKIAFTPAGVLVALTFIGLPFVVRT
VQPVLEDFEREQEEAAACLGASRWLTFRRVVLPAVLPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
QYDYAGATALAVVMLVVSFLMLLLINTLQWYLQRRTSKGASGPAPATVTAAAAAAGGQ
>Mature_297_residues
TTYTFRKPSALPGFGVTLGITVAYLSLVVLIPLAATFLKTATLSWDQFVTAVTSPRVLASYRLTFSAALGGALINAVFGF
LVAWVLVRYTFPFKRLVDAVVDLPFALPTSVAGISLAAVYATNGWVGQYLAPLGIKIAFTPAGVLVALTFIGLPFVVRTV
QPVLEDFEREQEEAAACLGASRWLTFRRVVLPAVLPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLEQ
YDYAGATALAVVMLVVSFLMLLLINTLQWYLQRRTSKGASGPAPATVTAAAAAAGGQ

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=264, Percent_Identity=50, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI87082099, Length=262, Percent_Identity=33.969465648855, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786980, Length=202, Percent_Identity=34.1584158415842, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1787369, Length=201, Percent_Identity=27.8606965174129, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786254, Length=227, Percent_Identity=28.1938325991189, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787368, Length=245, Percent_Identity=27.3469387755102, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31645; Mature: 31514

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTYTFRKPSALPGFGVTLGITVAYLSLVVLIPLAATFLKTATLSWDQFVTAVTSPRVLA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHH
SYRLTFSAALGGALINAVFGFLVAWVLVRYTFPFKRLVDAVVDLPFALPTSVAGISLAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
YATNGWVGQYLAPLGIKIAFTPAGVLVALTFIGLPFVVRTVQPVLEDFEREQEEAAACLG
HCCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASRWLTFRRVVLPAVLPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
QYDYAGATALAVVMLVVSFLMLLLINTLQWYLQRRTSKGASGPAPATVTAAAAAAGGQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
TTYTFRKPSALPGFGVTLGITVAYLSLVVLIPLAATFLKTATLSWDQFVTAVTSPRVLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHH
SYRLTFSAALGGALINAVFGFLVAWVLVRYTFPFKRLVDAVVDLPFALPTSVAGISLAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
YATNGWVGQYLAPLGIKIAFTPAGVLVALTFIGLPFVVRTVQPVLEDFEREQEEAAACLG
HCCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASRWLTFRRVVLPAVLPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
QYDYAGATALAVVMLVVSFLMLLLINTLQWYLQRRTSKGASGPAPATVTAAAAAAGGQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]