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The map label for this gene is lexA

Identifier: 116689622

GI number: 116689622

Start: 1759783

End: 1760430

Strand: Reverse

Name: lexA

Synonym: Bcen2424_1600

Alternate gene names: 116689622

Gene position: 1760430-1759783 (Counterclockwise)

Preceding gene: 116689642

Following gene: 116689621

Centisome position: 50.53

GC content: 68.21

Gene sequence:

>648_bases
ATGACCAAACTCACCGCCCGTCAGCAGCAAGTGTTCGACTTGATCCGTCGCGCGATCGAGCGCTCCGGATTCCCGCCCAC
CCGTGCCGAGATCGCGGCCGAACTGGGCTTCAGCTCGCCGAATGCGGCCGAGGAGCACCTGCGCGCGCTGGCTCGCAAGG
GCGTGATCGAGCTGGCCGCCGGCGCATCGCGCGGCATCCGGCTGCTCGGCATCGACGACGCCCCGCACCAGTTCACGCTG
CCGCATGCCGGCCTGATGCAACTGTCGCTGCCGCTCGTCGGCCGCGTCGCGGCCGGTAGCCCGATCCTTGCGCAGGAGCA
CATCTCGCAGCACTACGCGTGCGATCCCGCGCTGTTCACCAGCAAGCCCGACTATCTGTTGAAGGTGCGCGGCCTGTCGA
TGCGCGACGCCGGCATTCTCGACGGCGACCTCCTCGCCGTGCAGAAGCGCACGGAAGCGAAGGACGGCCAGATCATCGTC
GCGCGTCTCGGCGACGACGTCACGGTCAAGCGGCTGATGCGCCGGCCCGGCGGCCTCGAGCTGATCGCGGAGAACCCGGA
TTACGAAAACATCTTCGTCAAGGCCGGCAGCGCGGATTTCGCGCTGGAAGGCATCGCCGTCGGGCTGATCCGCTCGGGCG
AACTCTGA

Upstream 100 bases:

>100_bases
CGCCGGAACCGCCGCGATCGCGTCGAACCTCAAGTAACACTTGAAATTCGCCAAGTACTGTATAAAAATACAGCTCACTG
TCTATCCATACAGTTGAGCC

Downstream 100 bases:

>100_bases
CATTCCGTCTCTCCAGGAGAACATCATGGAACGCCTTGCCCGCCTGCTGCCTTTCCGTCAATTTGGTCGCCTGCGTCATC
TGCGCGGCCGCACGCCCTGC

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MTKLTARQQQVFDLIRRAIERSGFPPTRAEIAAELGFSSPNAAEEHLRALARKGVIELAAGASRGIRLLGIDDAPHQFTL
PHAGLMQLSLPLVGRVAAGSPILAQEHISQHYACDPALFTSKPDYLLKVRGLSMRDAGILDGDLLAVQKRTEAKDGQIIV
ARLGDDVTVKRLMRRPGGLELIAENPDYENIFVKAGSADFALEGIAVGLIRSGEL

Sequences:

>Translated_215_residues
MTKLTARQQQVFDLIRRAIERSGFPPTRAEIAAELGFSSPNAAEEHLRALARKGVIELAAGASRGIRLLGIDDAPHQFTL
PHAGLMQLSLPLVGRVAAGSPILAQEHISQHYACDPALFTSKPDYLLKVRGLSMRDAGILDGDLLAVQKRTEAKDGQIIV
ARLGDDVTVKRLMRRPGGLELIAENPDYENIFVKAGSADFALEGIAVGLIRSGEL
>Mature_214_residues
TKLTARQQQVFDLIRRAIERSGFPPTRAEIAAELGFSSPNAAEEHLRALARKGVIELAAGASRGIRLLGIDDAPHQFTLP
HAGLMQLSLPLVGRVAAGSPILAQEHISQHYACDPALFTSKPDYLLKVRGLSMRDAGILDGDLLAVQKRTEAKDGQIIVA
RLGDDVTVKRLMRRPGGLELIAENPDYENIFVKAGSADFALEGIAVGLIRSGEL

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=215, Percent_Identity=55.8139534883721, Blast_Score=239, Evalue=7e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_BURCA (Q1BWH8)

Other databases:

- EMBL:   CP000378
- RefSeq:   YP_621000.1
- ProteinModelPortal:   Q1BWH8
- STRING:   Q1BWH8
- MEROPS:   S24.001
- GeneID:   4093780
- GenomeReviews:   CP000378_GR
- KEGG:   bcn:Bcen_1120
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- ProtClustDB:   PRK00215
- BioCyc:   BCEN331271:BCEN_1120-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 23180; Mature: 23049

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: ACT_SITE 133-133 ACT_SITE 170-170

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLTARQQQVFDLIRRAIERSGFPPTRAEIAAELGFSSPNAAEEHLRALARKGVIELAA
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEC
GASRGIRLLGIDDAPHQFTLPHAGLMQLSLPLVGRVAAGSPILAQEHISQHYACDPALFT
CCCCCEEEEECCCCCCEEECCCCCHHHHHCCHHHHHCCCCCCHHHHHHHHHCCCCCHHCC
SKPDYLLKVRGLSMRDAGILDGDLLAVQKRTEAKDGQIIVARLGDDVTVKRLMRRPGGLE
CCCCEEEEEECCCCCCCCCCCCHHHEEEHHCCCCCCCEEEEECCCCHHHHHHHCCCCCEE
LIAENPDYENIFVKAGSADFALEGIAVGLIRSGEL
EEECCCCCCEEEEECCCCCEEHHHEEEEEEECCCC
>Mature Secondary Structure 
TKLTARQQQVFDLIRRAIERSGFPPTRAEIAAELGFSSPNAAEEHLRALARKGVIELAA
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEC
GASRGIRLLGIDDAPHQFTLPHAGLMQLSLPLVGRVAAGSPILAQEHISQHYACDPALFT
CCCCCEEEEECCCCCCEEECCCCCHHHHHCCHHHHHCCCCCCHHHHHHHHHCCCCCHHCC
SKPDYLLKVRGLSMRDAGILDGDLLAVQKRTEAKDGQIIVARLGDDVTVKRLMRRPGGLE
CCCCEEEEEECCCCCCCCCCCCHHHEEEHHCCCCCCCEEEEECCCCHHHHHHHCCCCCEE
LIAENPDYENIFVKAGSADFALEGIAVGLIRSGEL
EEECCCCCCEEEEECCCCCEEHHHEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA