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The map label for this gene is citH [H]

Identifier: 116689611

GI number: 116689611

Start: 1749312

End: 1750598

Strand: Reverse

Name: citH [H]

Synonym: Bcen2424_1589

Alternate gene names: 116689611

Gene position: 1750598-1749312 (Counterclockwise)

Preceding gene: 116689612

Following gene: 116689608

Centisome position: 50.25

GC content: 67.83

Gene sequence:

>1287_bases
ATGAATACGACCACCCTGACCTCGCAGGACGCCGCGCGCCCCAGCGCCGCGAAGATCCGGCGCATCATCTTCGCGGCGTC
GATCGGCAACGCACTCGAATGGTTCGACCTGATCGTCTACGGCTTCTTCGCGGTCACGATCGCCAAGCTGTTCTTCCCCG
CCACGAGCGAAGCGACGTCGCTGATGCTGACGCTCGGCACGTTCGGGCTGTCCTACCTGATCCGGCCGATCGGCGGCTTC
GTGCTCGGCGCGTACGCGGACCGCGCGGGCCGCAAGGCGTCGCTGCTGCTGTCGATCGCGATGATGATGGCCGGCACGCT
GCTGATCGCGCTGATGCCGACCTACGCATCGATCGGCATGCTCGCGCCGCTCGGGATCATGCTGTCGCGGCTGATGCAGG
GCTTTTCCGCCGGCGGCGAATTCGCGAGTTCGACCGCGTTTCTCGTCGAGCACGCGCCGCAGCGGCGCGGCTTCATGTCG
AGCTGGCAGTTCGCGAGCCAGGGCCTCGCGACGTTGCTCGCGTCGGGCTTCGGCGCGCTGCTGACGGCCACGCTGACCAC
CGCGCAACTCGAAAGCTGGGGCTGGCGCGTGCCGTTCCTGTTCGGCCTCGCGATCGGCCCGGTCGGGCTGTACATCCGCC
GCTACGTCGACGAAGGCGTCGAGTTCAAGACGCAGGCGCGCTCCGAAGCGCCGGTGCGCGAACTGTTCGCGGATCAGAAG
ATGCGGCTGCTGCTGTCGGTCGGCGCGCTCGTGATCTCGACCGCGATCAACTACATGATCCTGTACATGCCGACCTACGC
GATCAAGCAGCTCGGGCTGCCCGCATCGACCGGTTTCGCGGCGACGCTCGCGACCGGCTTCGTGCTGACGCTCGTCACGC
CGGTCGTCGGCCATCTGTCCGACAGCACCGGGCGAATCCGCATGATGGCGGTCGCGGCCGTGCTGATGCTCCTGACCGTG
TGGCCGACGTTCGCCTGGCTCACGCGCCACGCGTCGTTCGCGACGATGCTCGCCGCACTGGTCTGGATCGGCGCGCTGAA
GGCGATGTACTGCGGCGCGCTGCCGGCGCTGATGGCCGAACTGTTCCCGTCGCAGACGCGCGCCACCGGGCTCGCGGTCA
GCTACAACACCGGCGTCACGCTGTTCGGCGGCTTCGCGCCGTTCGTCATCACCTGGTTGATCAGCACGACCGGCAACCGG
CTGTCGCCGGCGCTCTATCTGATGGGATGTGCGCTGCTGAGCCTCGCCGCACTGTTCGTCGCGCGCACGCGGCTGAAAAT
GCGCTGA

Upstream 100 bases:

>100_bases
TCTCGGCGACGACGCGCCGCCCGTTCGCCGCGCGGCATCCGCCGCGGCCGGCACCGCCCTGTAACGCGCCTCGCCCCTTT
TCACGGGACCTGCACACACG

Downstream 100 bases:

>100_bases
CGCGAGAAACGGCGGCGCAGCAAGGCGCCGCCGAAACCGCCGGACTCGGTGCGACTCGGCCCGTCGCGGCATCGCTGCCG
CGCGGGCCGCGCGCCGTTTA

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNTTTLTSQDAARPSAAKIRRIIFAASIGNALEWFDLIVYGFFAVTIAKLFFPATSEATSLMLTLGTFGLSYLIRPIGGF
VLGAYADRAGRKASLLLSIAMMMAGTLLIALMPTYASIGMLAPLGIMLSRLMQGFSAGGEFASSTAFLVEHAPQRRGFMS
SWQFASQGLATLLASGFGALLTATLTTAQLESWGWRVPFLFGLAIGPVGLYIRRYVDEGVEFKTQARSEAPVRELFADQK
MRLLLSVGALVISTAINYMILYMPTYAIKQLGLPASTGFAATLATGFVLTLVTPVVGHLSDSTGRIRMMAVAAVLMLLTV
WPTFAWLTRHASFATMLAALVWIGALKAMYCGALPALMAELFPSQTRATGLAVSYNTGVTLFGGFAPFVITWLISTTGNR
LSPALYLMGCALLSLAALFVARTRLKMR

Sequences:

>Translated_428_residues
MNTTTLTSQDAARPSAAKIRRIIFAASIGNALEWFDLIVYGFFAVTIAKLFFPATSEATSLMLTLGTFGLSYLIRPIGGF
VLGAYADRAGRKASLLLSIAMMMAGTLLIALMPTYASIGMLAPLGIMLSRLMQGFSAGGEFASSTAFLVEHAPQRRGFMS
SWQFASQGLATLLASGFGALLTATLTTAQLESWGWRVPFLFGLAIGPVGLYIRRYVDEGVEFKTQARSEAPVRELFADQK
MRLLLSVGALVISTAINYMILYMPTYAIKQLGLPASTGFAATLATGFVLTLVTPVVGHLSDSTGRIRMMAVAAVLMLLTV
WPTFAWLTRHASFATMLAALVWIGALKAMYCGALPALMAELFPSQTRATGLAVSYNTGVTLFGGFAPFVITWLISTTGNR
LSPALYLMGCALLSLAALFVARTRLKMR
>Mature_428_residues
MNTTTLTSQDAARPSAAKIRRIIFAASIGNALEWFDLIVYGFFAVTIAKLFFPATSEATSLMLTLGTFGLSYLIRPIGGF
VLGAYADRAGRKASLLLSIAMMMAGTLLIALMPTYASIGMLAPLGIMLSRLMQGFSAGGEFASSTAFLVEHAPQRRGFMS
SWQFASQGLATLLASGFGALLTATLTTAQLESWGWRVPFLFGLAIGPVGLYIRRYVDEGVEFKTQARSEAPVRELFADQK
MRLLLSVGALVISTAINYMILYMPTYAIKQLGLPASTGFAATLATGFVLTLVTPVVGHLSDSTGRIRMMAVAAVLMLLTV
WPTFAWLTRHASFATMLAALVWIGALKAMYCGALPALMAELFPSQTRATGLAVSYNTGVTLFGGFAPFVITWLISTTGNR
LSPALYLMGCALLSLAALFVARTRLKMR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=425, Percent_Identity=32.7058823529412, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1788942, Length=402, Percent_Identity=35.8208955223881, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1788292, Length=436, Percent_Identity=29.1284403669725, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1789941, Length=430, Percent_Identity=31.6279069767442, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI87082231, Length=436, Percent_Identity=24.3119266055046, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082404, Length=423, Percent_Identity=24.3498817966903, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45899; Mature: 45899

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTTLTSQDAARPSAAKIRRIIFAASIGNALEWFDLIVYGFFAVTIAKLFFPATSEATS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LMLTLGTFGLSYLIRPIGGFVLGAYADRAGRKASLLLSIAMMMAGTLLIALMPTYASIGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAPLGIMLSRLMQGFSAGGEFASSTAFLVEHAPQRRGFMSSWQFASQGLATLLASGFGAL
HHHHHHHHHHHHHHHCCCCCHHCCHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LTATLTTAQLESWGWRVPFLFGLAIGPVGLYIRRYVDEGVEFKTQARSEAPVRELFADQK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHH
MRLLLSVGALVISTAINYMILYMPTYAIKQLGLPASTGFAATLATGFVLTLVTPVVGHLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
DSTGRIRMMAVAAVLMLLTVWPTFAWLTRHASFATMLAALVWIGALKAMYCGALPALMAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFPSQTRATGLAVSYNTGVTLFGGFAPFVITWLISTTGNRLSPALYLMGCALLSLAALFV
HCCCCHHHCCEEEEECCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ARTRLKMR
HHHHHCCC
>Mature Secondary Structure
MNTTTLTSQDAARPSAAKIRRIIFAASIGNALEWFDLIVYGFFAVTIAKLFFPATSEATS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LMLTLGTFGLSYLIRPIGGFVLGAYADRAGRKASLLLSIAMMMAGTLLIALMPTYASIGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAPLGIMLSRLMQGFSAGGEFASSTAFLVEHAPQRRGFMSSWQFASQGLATLLASGFGAL
HHHHHHHHHHHHHHHCCCCCHHCCHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LTATLTTAQLESWGWRVPFLFGLAIGPVGLYIRRYVDEGVEFKTQARSEAPVRELFADQK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHH
MRLLLSVGALVISTAINYMILYMPTYAIKQLGLPASTGFAATLATGFVLTLVTPVVGHLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
DSTGRIRMMAVAAVLMLLTVWPTFAWLTRHASFATMLAALVWIGALKAMYCGALPALMAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFPSQTRATGLAVSYNTGVTLFGGFAPFVITWLISTTGNRLSPALYLMGCALLSLAALFV
HCCCCHHHCCEEEEECCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ARTRLKMR
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]