BacMap

Definition	Burkholderia cenocepacia HI2424 chromosome 1, complete sequence.
Accession	NC_008542
Length	3,483,902

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The map label for this gene is braG [H]

Identifier: 116689439

GI number: 116689439

Start: 1563104

End: 1563808

Strand: Direct

Name: braG [H]

Synonym: Bcen2424_1417

Alternate gene names: 116689439

Gene position: 1563104-1563808 (Clockwise)

Preceding gene: 116689438

Following gene: 116689440

Centisome position: 44.87

GC content: 73.33

Gene sequence:

>705_bases
ATGAACGCCGTGCTCGACATTCGCGGGCTGCGCGCGTGGTACGGGAGGCAGCCCGTGCTCGACGGTGTCGATCTCGCGCT
CGCGCCGGGCGAGACGCTCGCGCTGCTCGGCCGCAACGGCTCGGGACGCTCGACACTCGCGAAGGCCGTGATGGGGCTCG
TACGCACGGCCGGCTCGGTGCGCATCGCCGGCGCCGAATGCGCGGGGGCGCGCACGTTCGAGATCGCGCGACGCGGCGTT
GCTTACGTGGCTGAAAGCCGCGACGTGTTTCCGCTGCTGAGCGTGCGCGACAACCTGCGGCTCGGTTTGCGCGGCACAAG
CGGCACCGCCGAACGCACGGCGCTCGATCGTCTGTTCGAGCGTTTTCCGCTGCTGGCCGCGCGCGCGGACGTGAAAGCCG
GGCGGCTGTCGGGCGGCGAGCAGCAGGTGCTCGCGCTGGTGCGCGCACTCGCCGGCCGCCCGCGCGTGCTGATCGTCGAC
GAGCCGGCCGAGGGGCTTGCGCCGCTCGCGGTCGACGAAGTCGGCGCATGCCTCGCCGCGCTGCAGGCCGATGGCGTCGC
GATCGTGCTGATCGAGCAGCGTCTGCAGCTCGCGCCGCGGCTCGCGCGGCGCGTTGCGGTGATGGGGCACGGACGGATCG
TCTACGACGGCGCGCTCGACGGGCTGGGCGGCGAGGTCGCCGATGCGTGGTTGAGCGCCGGCTGA

Upstream 100 bases:

>100_bases
TCACGGTGCTCGTGCGCGGCGCGGTGGTCGCGACCGGCGCGCCCGATGCGATCCGCGCCGATCCGAGCGTGCGTGCCGCG
TACCTGGGCGAGGGCGCGGC

Downstream 100 bases:

>100_bases
CCGCGCCCGGTCGATTGTTCGGCAAACTCCGCCAGTCAATTCGCATCGAGCCGCTTTATCGCTGCGGTGCCGCAACGTAC
ATTGCCAGTCAGGCCGGTCG

Product: ABC transporter related

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MNAVLDIRGLRAWYGRQPVLDGVDLALAPGETLALLGRNGSGRSTLAKAVMGLVRTAGSVRIAGAECAGARTFEIARRGV
AYVAESRDVFPLLSVRDNLRLGLRGTSGTAERTALDRLFERFPLLAARADVKAGRLSGGEQQVLALVRALAGRPRVLIVD
EPAEGLAPLAVDEVGACLAALQADGVAIVLIEQRLQLAPRLARRVAVMGHGRIVYDGALDGLGGEVADAWLSAG

Sequences:

>Translated_234_residues
MNAVLDIRGLRAWYGRQPVLDGVDLALAPGETLALLGRNGSGRSTLAKAVMGLVRTAGSVRIAGAECAGARTFEIARRGV
AYVAESRDVFPLLSVRDNLRLGLRGTSGTAERTALDRLFERFPLLAARADVKAGRLSGGEQQVLALVRALAGRPRVLIVD
EPAEGLAPLAVDEVGACLAALQADGVAIVLIEQRLQLAPRLARRVAVMGHGRIVYDGALDGLGGEVADAWLSAG
>Mature_234_residues
MNAVLDIRGLRAWYGRQPVLDGVDLALAPGETLALLGRNGSGRSTLAKAVMGLVRTAGSVRIAGAECAGARTFEIARRGV
AYVAESRDVFPLLSVRDNLRLGLRGTSGTAERTALDRLFERFPLLAARADVKAGRLSGGEQQVLALVRALAGRPRVLIVD
EPAEGLAPLAVDEVGACLAALQADGVAIVLIEQRLQLAPRLARRVAVMGHGRIVYDGALDGLGGEVADAWLSAG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI153792144, Length=207, Percent_Identity=29.4685990338164, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI150417984, Length=233, Percent_Identity=33.0472103004292, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI45446740, Length=237, Percent_Identity=27.4261603375527, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI47078218, Length=237, Percent_Identity=27.4261603375527, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI27477115, Length=219, Percent_Identity=29.2237442922374, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI27436953, Length=219, Percent_Identity=28.7671232876712, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI21536376, Length=237, Percent_Identity=26.1603375527426, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI105990541, Length=217, Percent_Identity=26.7281105990783, Blast_Score=74, Evalue=7e-14,
Organism=Homo sapiens, GI90421313, Length=199, Percent_Identity=27.1356783919598, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI6005701, Length=217, Percent_Identity=29.9539170506912, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI87082268, Length=220, Percent_Identity=35, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1789672, Length=226, Percent_Identity=30.5309734513274, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788225, Length=230, Percent_Identity=31.304347826087, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787029, Length=220, Percent_Identity=30.4545454545455, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1786872, Length=226, Percent_Identity=31.858407079646, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787792, Length=221, Percent_Identity=29.8642533936652, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1787089, Length=223, Percent_Identity=30.9417040358744, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87081791, Length=221, Percent_Identity=30.7692307692308, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1786703, Length=211, Percent_Identity=33.6492890995261, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1790544, Length=248, Percent_Identity=28.6290322580645, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788761, Length=231, Percent_Identity=30.7359307359307, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788897, Length=221, Percent_Identity=29.8642533936652, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1789593, Length=212, Percent_Identity=29.7169811320755, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1789864, Length=234, Percent_Identity=28.2051282051282, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1790190, Length=208, Percent_Identity=31.25, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1789032, Length=215, Percent_Identity=33.953488372093, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1787370, Length=222, Percent_Identity=31.5315315315315, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI87081782, Length=232, Percent_Identity=29.3103448275862, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788450, Length=219, Percent_Identity=30.1369863013699, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1788472, Length=226, Percent_Identity=25.2212389380531, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI48995001, Length=216, Percent_Identity=29.6296296296296, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789991, Length=220, Percent_Identity=26.8181818181818, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787712, Length=217, Percent_Identity=29.0322580645161, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1790525, Length=226, Percent_Identity=28.3185840707965, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1789891, Length=233, Percent_Identity=33.9055793991416, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1786563, Length=212, Percent_Identity=30.188679245283, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790162, Length=232, Percent_Identity=25.8620689655172, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1786319, Length=227, Percent_Identity=28.1938325991189, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786253, Length=206, Percent_Identity=29.126213592233, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081834, Length=211, Percent_Identity=30.3317535545024, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1789963, Length=236, Percent_Identity=29.6610169491525, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787500, Length=234, Percent_Identity=28.6324786324786, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788210, Length=215, Percent_Identity=27.4418604651163, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789873, Length=208, Percent_Identity=26.9230769230769, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87081709, Length=166, Percent_Identity=30.1204819277108, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786398, Length=201, Percent_Identity=29.8507462686567, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI145693107, Length=211, Percent_Identity=32.2274881516588, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1787164, Length=209, Percent_Identity=33.0143540669856, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1787547, Length=233, Percent_Identity=26.1802575107296, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI48994943, Length=217, Percent_Identity=29.0322580645161, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082267, Length=168, Percent_Identity=32.1428571428571, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788165, Length=177, Percent_Identity=32.7683615819209, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786698, Length=148, Percent_Identity=31.0810810810811, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787758, Length=218, Percent_Identity=27.9816513761468, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787105, Length=225, Percent_Identity=28.4444444444444, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1787041, Length=213, Percent_Identity=26.7605633802817, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1788506, Length=225, Percent_Identity=31.5555555555556, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788644, Length=236, Percent_Identity=26.6949152542373, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787113, Length=201, Percent_Identity=31.3432835820896, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790467, Length=159, Percent_Identity=32.0754716981132, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787112, Length=209, Percent_Identity=30.1435406698565, Blast_Score=62, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=221, Percent_Identity=29.8642533936652, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115533608, Length=221, Percent_Identity=28.5067873303167, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983523, Length=201, Percent_Identity=30.8457711442786, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71983516, Length=201, Percent_Identity=30.8457711442786, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71983510, Length=201, Percent_Identity=30.8457711442786, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17565586, Length=204, Percent_Identity=28.4313725490196, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193209708, Length=218, Percent_Identity=28.4403669724771, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17565938, Length=206, Percent_Identity=30.5825242718447, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193208177, Length=220, Percent_Identity=26.3636363636364, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71992290, Length=203, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17558664, Length=214, Percent_Identity=27.5700934579439, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193211017, Length=221, Percent_Identity=28.0542986425339, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193211015, Length=221, Percent_Identity=28.0542986425339, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71996809, Length=221, Percent_Identity=28.5067873303167, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17543740, Length=211, Percent_Identity=27.9620853080569, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25146777, Length=228, Percent_Identity=27.1929824561404, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI212646699, Length=215, Percent_Identity=27.4418604651163, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17541710, Length=230, Percent_Identity=28.2608695652174, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24666092, Length=228, Percent_Identity=29.3859649122807, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24641565, Length=223, Percent_Identity=31.390134529148, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24643674, Length=220, Percent_Identity=28.1818181818182, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28574150, Length=218, Percent_Identity=27.9816513761468, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI116007328, Length=218, Percent_Identity=27.9816513761468, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24581191, Length=210, Percent_Identity=27.6190476190476, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24581189, Length=210, Percent_Identity=27.6190476190476, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24581193, Length=210, Percent_Identity=27.6190476190476, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24581195, Length=210, Percent_Identity=27.6190476190476, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI221512771, Length=215, Percent_Identity=27.4418604651163, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648314, Length=218, Percent_Identity=26.605504587156, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI116007184, Length=195, Percent_Identity=28.2051282051282, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221500365, Length=195, Percent_Identity=28.2051282051282, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28574744, Length=213, Percent_Identity=27.2300469483568, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574533, Length=217, Percent_Identity=27.6497695852535, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19920532, Length=222, Percent_Identity=28.3783783783784, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580930, Length=222, Percent_Identity=28.3783783783784, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI23397593, Length=216, Percent_Identity=29.1666666666667, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 24477; Mature: 24477

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVLDIRGLRAWYGRQPVLDGVDLALAPGETLALLGRNGSGRSTLAKAVMGLVRTAGSV
CCCEEECHHHHHHHCCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCE
RIAGAECAGARTFEIARRGVAYVAESRDVFPLLSVRDNLRLGLRGTSGTAERTALDRLFE
EEECCCCCCCHHHHHHHHHHHEEECCCCCEEHEECCCCCEEEEECCCCCHHHHHHHHHHH
RFPLLAARADVKAGRLSGGEQQVLALVRALAGRPRVLIVDEPAEGLAPLAVDEVGACLAA
HCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
LQADGVAIVLIEQRLQLAPRLARRVAVMGHGRIVYDGALDGLGGEVADAWLSAG
HHCCCEEEEEEHHHHHHHHHHHHHHHHEECCEEEECCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MNAVLDIRGLRAWYGRQPVLDGVDLALAPGETLALLGRNGSGRSTLAKAVMGLVRTAGSV
CCCEEECHHHHHHHCCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCE
RIAGAECAGARTFEIARRGVAYVAESRDVFPLLSVRDNLRLGLRGTSGTAERTALDRLFE
EEECCCCCCCHHHHHHHHHHHEEECCCCCEEHEECCCCCEEEEECCCCCHHHHHHHHHHH
RFPLLAARADVKAGRLSGGEQQVLALVRALAGRPRVLIVDEPAEGLAPLAVDEVGACLAA
HCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
LQADGVAIVLIEQRLQLAPRLARRVAVMGHGRIVYDGALDGLGGEVADAWLSAG
HHCCCEEEEEEHHHHHHHHHHHHHHHHEECCEEEECCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]