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The map label for this gene is yneJ [H]

Identifier: 116689432

GI number: 116689432

Start: 1556642

End: 1557556

Strand: Reverse

Name: yneJ [H]

Synonym: Bcen2424_1410

Alternate gene names: 116689432

Gene position: 1557556-1556642 (Counterclockwise)

Preceding gene: 116689441

Following gene: 116689430

Centisome position: 44.71

GC content: 72.46

Gene sequence:

>915_bases
ATGGACCTGGCGGCGCTGGCGATTTTCCGGGCGGTCGTGCGCGAAAACGGCGTGACGCGCGCGGCGGCCAAGCTCAATCG
CGTGCAGTCGAACGTCACGACCCGCATCAAGCAGCTGGAGGAGGAACTCGGCGCGGCGCTGTTCGTGCGCGACGGCCGCC
GGCTCGTGCTGACGCCGGCCGGGCACACGCTGCTGCCGTATGCGGAGCGCCTGCTCACGCTCGCCGACGAGGCACGCGAC
GCGGTGCGCGAGGACACGCCGCGCGGGCGGCTGCGGCTCGGCACGATGGAAAGCACGGCCGCGAGCCGGCTGCCGACCCT
CCTCGCGCGCTACCACCATGCGTGGCCCGACGTGTCGCTCGAGCTGCTGACGGGCACGACGGGCTACCTGATCGACCGCG
TGCGCGACTTCGAGATCGACGCCGCGCTGTTCGCGCGGCCGCCCGAGCCGGACACGCTGCCCGACACGTTCGAGACGGTG
CCGATCTTCCGCGAGGATCTCGTGCTGCTCACGCCGCGCGGCCATCCGCCGGTGCGCACGCCGCGCGACGTGATCCTGCC
GACGCTGATCGCGTTCGAGCGCGGCTGCGCGTATCGCAAGTACGTCGAACGGTGGTACGCGGCGCACGGCGTGAAGCCGG
CCCGGGTGCTCGAACTCGGCTCGTATCACGCGATCGTCGCGTGCGTCGCGGCCGGCGCGGGCGTCGCGGTCGCGCCACGT
TCGGTACTCGATCTGCAGCCGGAGACCGGCAACATCGCCGCGCACACGATTCCCGAACTCGAAGGCATCGACACGCTGCT
CGGGTGGCGGCGCGGCTATGCGTCGGCGGCGCTCGCCGCGCTGCGCGATGCGCTCACCGACGCCGCGCGGCAGCCTGGGG
ACATGACGACGCCAACGGTGACCGTGCCGGCATGA

Upstream 100 bases:

>100_bases
GCGCCGGCCGGATCGGAAATCGGAATGGGTCCATCCTAGCTTGACCATCCGTTCTCGAAAAATGAATAATTCAGAATCGA
CTCATCCCTCGGAGAGAATC

Downstream 100 bases:

>100_bases
CCCCTATGCGCGCGCCGCCGCATCGATCGACGCGCATGGGCTGCACAAACGAAACGACCCGACCGGCGCCTGCCGATCGG
GTCGTTCGTCGATTCACGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEELGAALFVRDGRRLVLTPAGHTLLPYAERLLTLADEARD
AVREDTPRGRLRLGTMESTAASRLPTLLARYHHAWPDVSLELLTGTTGYLIDRVRDFEIDAALFARPPEPDTLPDTFETV
PIFREDLVLLTPRGHPPVRTPRDVILPTLIAFERGCAYRKYVERWYAAHGVKPARVLELGSYHAIVACVAAGAGVAVAPR
SVLDLQPETGNIAAHTIPELEGIDTLLGWRRGYASAALAALRDALTDAARQPGDMTTPTVTVPA

Sequences:

>Translated_304_residues
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEELGAALFVRDGRRLVLTPAGHTLLPYAERLLTLADEARD
AVREDTPRGRLRLGTMESTAASRLPTLLARYHHAWPDVSLELLTGTTGYLIDRVRDFEIDAALFARPPEPDTLPDTFETV
PIFREDLVLLTPRGHPPVRTPRDVILPTLIAFERGCAYRKYVERWYAAHGVKPARVLELGSYHAIVACVAAGAGVAVAPR
SVLDLQPETGNIAAHTIPELEGIDTLLGWRRGYASAALAALRDALTDAARQPGDMTTPTVTVPA
>Mature_304_residues
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEELGAALFVRDGRRLVLTPAGHTLLPYAERLLTLADEARD
AVREDTPRGRLRLGTMESTAASRLPTLLARYHHAWPDVSLELLTGTTGYLIDRVRDFEIDAALFARPPEPDTLPDTFETV
PIFREDLVLLTPRGHPPVRTPRDVILPTLIAFERGCAYRKYVERWYAAHGVKPARVLELGSYHAIVACVAAGAGVAVAPR
SVLDLQPETGNIAAHTIPELEGIDTLLGWRRGYASAALAALRDALTDAARQPGDMTTPTVTVPA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=294, Percent_Identity=40.8163265306122, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI87082132, Length=259, Percent_Identity=30.5019305019305, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788481, Length=291, Percent_Identity=30.2405498281787, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790208, Length=256, Percent_Identity=30.46875, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788887, Length=293, Percent_Identity=28.3276450511945, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788296, Length=246, Percent_Identity=30.4878048780488, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI157672245, Length=229, Percent_Identity=27.9475982532751, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787879, Length=260, Percent_Identity=28.0769230769231, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693105, Length=244, Percent_Identity=24.1803278688525, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790262, Length=297, Percent_Identity=25.9259259259259, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788626, Length=140, Percent_Identity=33.5714285714286, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787601, Length=172, Percent_Identity=26.1627906976744, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI2367136, Length=246, Percent_Identity=26.4227642276423, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789204, Length=252, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788748, Length=298, Percent_Identity=25.8389261744966, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787530, Length=282, Percent_Identity=25.177304964539, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI48994962, Length=154, Percent_Identity=31.1688311688312, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786401, Length=151, Percent_Identity=33.112582781457, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790399, Length=255, Percent_Identity=23.5294117647059, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33201; Mature: 33201

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEELGAALFVRDGRRLVLTPA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCC
GHTLLPYAERLLTLADEARDAVREDTPRGRLRLGTMESTAASRLPTLLARYHHAWPDVSL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEE
ELLTGTTGYLIDRVRDFEIDAALFARPPEPDTLPDTFETVPIFREDLVLLTPRGHPPVRT
EEECCCHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCCCCCC
PRDVILPTLIAFERGCAYRKYVERWYAAHGVKPARVLELGSYHAIVACVAAGAGVAVAPR
CHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCCCCEECCH
SVLDLQPETGNIAAHTIPELEGIDTLLGWRRGYASAALAALRDALTDAARQPGDMTTPTV
HHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
TVPA
EECC
>Mature Secondary Structure
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEELGAALFVRDGRRLVLTPA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCC
GHTLLPYAERLLTLADEARDAVREDTPRGRLRLGTMESTAASRLPTLLARYHHAWPDVSL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEE
ELLTGTTGYLIDRVRDFEIDAALFARPPEPDTLPDTFETVPIFREDLVLLTPRGHPPVRT
EEECCCHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCCCCCC
PRDVILPTLIAFERGCAYRKYVERWYAAHGVKPARVLELGSYHAIVACVAAGAGVAVAPR
CHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCCCCEECCH
SVLDLQPETGNIAAHTIPELEGIDTLLGWRRGYASAALAALRDALTDAARQPGDMTTPTV
HHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
TVPA
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]