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The map label for this gene is rstB [H]

Identifier: 116689373

GI number: 116689373

Start: 1489886

End: 1491271

Strand: Reverse

Name: rstB [H]

Synonym: Bcen2424_1351

Alternate gene names: 116689373

Gene position: 1491271-1489886 (Counterclockwise)

Preceding gene: 116689374

Following gene: 116689368

Centisome position: 42.8

GC content: 67.97

Gene sequence:

>1386_bases
ATGCTGCGCCCGTTGCTCCGGTTGTACCTCGTCGTGATCCTGTTCGTCGGTGCGTCGATCATCTTCATCCAGCTGTCGTT
CGACCGGATCTTCTATGAACGCGGCGCGCAGGCGCAGCGCGATTCGCTGACGACCTATTCGTTCGTGCTGAACGACTACC
TCGAACGCCATCCGGGCGCGCAGCGCGAGCTCGCATTGCGCGAACTCGCGCTGCATGGCCGCGAAGGATTCGGCTTCATG
TCGATGGCCGACGCGCGTGCGCAACTGAGCGGCGCGCCGTTGCGCGATCTCGATGCCGGCAGGATCGCGATCAGCTACAA
CGGCAAGGATTACTACATGCCGCTCGCGGACGGCTCGGTGCTGCATGCCCGCCCGATCGAAGCGCCCGACCTCGACATCC
GGATCTATGCGTACATGCTGCTCGCGCTCGCGACGCTGTTCGCGGTCGTGCTGTGGATTCACTATCACTGGCGCGACATT
CGCCGGCTGCAGGATGCCGCGCGCGCGTTCGGCGCCGGAATGCTGTCGACACGCGTGAAGCTGTCGGGCAAGTCGAACAT
CTACGAGCTGTCGCAGCAGTTCAACGACATGGCCGCGCGTATCGAGGCGTCGATCAAGCAGCAGCGCGAAATGATGCACG
GCATCTCGCATGAACTGAAAACGCCGCTCGCCCGGCTCGAATTCGGGCTCGCGCTGCTCGCCGACGCCGACGAGACGGGC
CGGATGCGCGAGCGCCGCGACGCGCTGCGACGCGACGTGCGCGAACTCGACGAGCTCGTCACCGAGTTGCTGACGATCGG
CCGGCTCGAACAGGGTGCGAGCGAGCTCGCGCCGATGGAAGTCGTCGTCGATGCGCTGATCGACAGCGTCGCCGGCAACG
TCGCGGACGACATCGCCGATCGCGGCATCGCGCTCGACGTGTCGACGTTCGACGCGCCCGCGACCCACGTCTGCGATCCG
AAGCTCGTCGCCCGCGCGCTGCTGAACCTGATTCGCAACGGCGCCCGCTATGCGTCGCGCAAGGTCGTGCTGGCCGCGAC
CCGCGACCCGTCCGGCGCGCTCGTGCTGAGCGTCGACGACGATGGCCCCGGCATTCCGGCCGCCGAACGCGCCCGCGTGT
TCGAGCCGTTCCAGCGGCTGGATTCGAGCCGCGACCGTCAGACCGGCGGTTTCGGGCTCGGGCTCGCGATCGTGCGGCGC
GTCGCGCAGGTGCACGGCGGCGACGTGCGGCTCGAGGATTCGCCGCTCGGCGGCGCGCGCTTCGTGATCACGCTGCCCGT
GCGTGCGCTGCCGGACAGTCTGTTCGACGTCGCGCAGCACGTGGAAAGCACGCATGCCGATGCGCGCCGCCGCAGCGGCA
CGGGCACGGTCGTCCCGCTTCGATGA

Upstream 100 bases:

>100_bases
GCTGCGCCGCCGCTTCGAGGATGCGTCATCGGAGCCGCACAAGATCAAGACGATCTGGGGTCGCGGCTACCTGTTCAGCC
CTTCTGCGTGGGACGAGTAA

Downstream 100 bases:

>100_bases
AAACGGCGGCGGGCGCCTACCCGCCCCCGCGTGACCGCGCCTTCGCTCAGCGCCGGCGCAACAGCGCGACGAAGAACAGG
CTGCCGAACAGCGCGGTCAC

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLRPLLRLYLVVILFVGASIIFIQLSFDRIFYERGAQAQRDSLTTYSFVLNDYLERHPGAQRELALRELALHGREGFGFM
SMADARAQLSGAPLRDLDAGRIAISYNGKDYYMPLADGSVLHARPIEAPDLDIRIYAYMLLALATLFAVVLWIHYHWRDI
RRLQDAARAFGAGMLSTRVKLSGKSNIYELSQQFNDMAARIEASIKQQREMMHGISHELKTPLARLEFGLALLADADETG
RMRERRDALRRDVRELDELVTELLTIGRLEQGASELAPMEVVVDALIDSVAGNVADDIADRGIALDVSTFDAPATHVCDP
KLVARALLNLIRNGARYASRKVVLAATRDPSGALVLSVDDDGPGIPAAERARVFEPFQRLDSSRDRQTGGFGLGLAIVRR
VAQVHGGDVRLEDSPLGGARFVITLPVRALPDSLFDVAQHVESTHADARRRSGTGTVVPLR

Sequences:

>Translated_461_residues
MLRPLLRLYLVVILFVGASIIFIQLSFDRIFYERGAQAQRDSLTTYSFVLNDYLERHPGAQRELALRELALHGREGFGFM
SMADARAQLSGAPLRDLDAGRIAISYNGKDYYMPLADGSVLHARPIEAPDLDIRIYAYMLLALATLFAVVLWIHYHWRDI
RRLQDAARAFGAGMLSTRVKLSGKSNIYELSQQFNDMAARIEASIKQQREMMHGISHELKTPLARLEFGLALLADADETG
RMRERRDALRRDVRELDELVTELLTIGRLEQGASELAPMEVVVDALIDSVAGNVADDIADRGIALDVSTFDAPATHVCDP
KLVARALLNLIRNGARYASRKVVLAATRDPSGALVLSVDDDGPGIPAAERARVFEPFQRLDSSRDRQTGGFGLGLAIVRR
VAQVHGGDVRLEDSPLGGARFVITLPVRALPDSLFDVAQHVESTHADARRRSGTGTVVPLR
>Mature_461_residues
MLRPLLRLYLVVILFVGASIIFIQLSFDRIFYERGAQAQRDSLTTYSFVLNDYLERHPGAQRELALRELALHGREGFGFM
SMADARAQLSGAPLRDLDAGRIAISYNGKDYYMPLADGSVLHARPIEAPDLDIRIYAYMLLALATLFAVVLWIHYHWRDI
RRLQDAARAFGAGMLSTRVKLSGKSNIYELSQQFNDMAARIEASIKQQREMMHGISHELKTPLARLEFGLALLADADETG
RMRERRDALRRDVRELDELVTELLTIGRLEQGASELAPMEVVVDALIDSVAGNVADDIADRGIALDVSTFDAPATHVCDP
KLVARALLNLIRNGARYASRKVVLAATRDPSGALVLSVDDDGPGIPAAERARVFEPFQRLDSSRDRQTGGFGLGLAIVRR
VAQVHGGDVRLEDSPLGGARFVITLPVRALPDSLFDVAQHVESTHADARRRSGTGTVVPLR

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=297, Percent_Identity=37.3737373737374, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1790346, Length=268, Percent_Identity=35.8208955223881, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789808, Length=293, Percent_Identity=30.7167235494881, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788393, Length=273, Percent_Identity=31.1355311355311, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1786600, Length=241, Percent_Identity=29.045643153527, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786912, Length=242, Percent_Identity=31.404958677686, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788713, Length=305, Percent_Identity=24.9180327868852, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786783, Length=302, Percent_Identity=30.4635761589404, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI145693157, Length=240, Percent_Identity=23.3333333333333, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1789149, Length=230, Percent_Identity=27.3913043478261, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI87082128, Length=296, Percent_Identity=27.3648648648649, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081816, Length=279, Percent_Identity=26.1648745519713, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790436, Length=218, Percent_Identity=28.8990825688073, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790551, Length=274, Percent_Identity=27.3722627737226, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788549, Length=247, Percent_Identity=23.8866396761134, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790861, Length=238, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787374, Length=300, Percent_Identity=23.6666666666667, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994928, Length=229, Percent_Identity=24.8908296943231, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789403, Length=236, Percent_Identity=26.271186440678, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50959; Mature: 50959

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRPLLRLYLVVILFVGASIIFIQLSFDRIFYERGAQAQRDSLTTYSFVLNDYLERHPGA
CCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHCCCC
QRELALRELALHGREGFGFMSMADARAQLSGAPLRDLDAGRIAISYNGKDYYMPLADGSV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEECCCCCE
LHARPIEAPDLDIRIYAYMLLALATLFAVVLWIHYHWRDIRRLQDAARAFGAGMLSTRVK
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEE
LSGKSNIYELSQQFNDMAARIEASIKQQREMMHGISHELKTPLARLEFGLALLADADETG
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCCHHH
RMRERRDALRRDVRELDELVTELLTIGRLEQGASELAPMEVVVDALIDSVAGNVADDIAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHHC
RGIALDVSTFDAPATHVCDPKLVARALLNLIRNGARYASRKVVLAATRDPSGALVLSVDD
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCCEEEEEECC
DGPGIPAAERARVFEPFQRLDSSRDRQTGGFGLGLAIVRRVAQVHGGDVRLEDSPLGGAR
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCEE
FVITLPVRALPDSLFDVAQHVESTHADARRRSGTGTVVPLR
EEEEECHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
>Mature Secondary Structure
MLRPLLRLYLVVILFVGASIIFIQLSFDRIFYERGAQAQRDSLTTYSFVLNDYLERHPGA
CCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHCCCC
QRELALRELALHGREGFGFMSMADARAQLSGAPLRDLDAGRIAISYNGKDYYMPLADGSV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEECCCCCE
LHARPIEAPDLDIRIYAYMLLALATLFAVVLWIHYHWRDIRRLQDAARAFGAGMLSTRVK
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEE
LSGKSNIYELSQQFNDMAARIEASIKQQREMMHGISHELKTPLARLEFGLALLADADETG
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCCHHH
RMRERRDALRRDVRELDELVTELLTIGRLEQGASELAPMEVVVDALIDSVAGNVADDIAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHHC
RGIALDVSTFDAPATHVCDPKLVARALLNLIRNGARYASRKVVLAATRDPSGALVLSVDD
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCCEEEEEECC
DGPGIPAAERARVFEPFQRLDSSRDRQTGGFGLGLAIVRRVAQVHGGDVRLEDSPLGGAR
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCEE
FVITLPVRALPDSLFDVAQHVESTHADARRRSGTGTVVPLR
EEEEECHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]