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The map label for this gene is insF1 [C]

Identifier: 116624244

GI number: 116624244

Start: 6487679

End: 6488521

Strand: Reverse

Name: insF1 [C]

Synonym: Acid_5161

Alternate gene names: 116624244

Gene position: 6488521-6487679 (Counterclockwise)

Preceding gene: 116624245

Following gene: 116624243

Centisome position: 65.11

GC content: 57.53

Gene sequence:

>843_bases
ATGAGTGTCGTGCAGATGTGCGAGTCGCTGGGCATCAGCCGGGCAGGATACTACCGCTTTTTGGATCCGGAAAAGCCGGC
ACCCGCCGATATGGATCTTCGCGACGAGATGCACCGGGTAGCCCTCGATTGGCCCTGCTATGGGAGCCGGCGTATCGTCG
AGGAACTCAAGGCTCGTGGATGGGAGGTCAACCGCAAGCGGATTCAGCGGCTGATGCGGGAAGACAACCTGCTGTGTGTG
ATCAAACGCAAGTTCGTCGTAGCCACCACCGATTCCCGTCACGGTCTGAAGGTATATCCGAATCGGGCCGCGGAGATGAC
GCTAACCGGAGTCGATCAGCTCTGGGTGGCCGATATCACCTACATCCGACTGGAAGAGGAGTTTGTCTACCTGGCGGTCA
TTCTGGATGCCTTCTCACGCCGGGTCATTGGCTGGCATTTGGGGGAAACCTTAGAAGTTTCCCTCACGCTGAATGCCTTG
CGCATGGCGCTTGGGCAACGATCGGTAAGTCCGGGCCTGGTTCACCACTCCGACCGCGGAGTCCAGTATGCCAGTCACGA
TTACACGCAACTGCTCCAGGACAACGGGATCGAAATCAGCATGAGTCGCAAGGCCAACCCCTGGGACAATGCGGCCTGCG
AGTCCTTCATGAAAACGCTGAAATACGAGGAGGTACACCGCACCGAATATCGGAATCTGGCACACGCCCGTGCGTCGATA
AAGACCTTCCTGGAGAAGATCTATAACCAAAAGCGATTGCACTCGTCCTTGAGCTATCGCTCGCCGGTCGAGTTCGAACA
CTCTCTAGCGGCCACCGCTTCTCAGCAGCAGGTGGCGGTATGA

Upstream 100 bases:

>100_bases
GGGCCTTGCAGCATGTCGAGGAACAGCGCCTGCTGCGGGCACTGAACAACGGCGCGCCCTCTACGAGCAGATCGACAAAG
AAGTCCAGGCGGAAACCCGC

Downstream 100 bases:

>100_bases
AAAGTGAGGGCGCCCTCAGGAAGCGTTTTCTCAGGCATGACGGAATCTATCGGTCCGATATGTCATTCCATCTGATAAAC
CCGGTCCAGCTGCCGCCTCC

Product: integrase catalytic subunit

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSVVQMCESLGISRAGYYRFLDPEKPAPADMDLRDEMHRVALDWPCYGSRRIVEELKARGWEVNRKRIQRLMREDNLLCV
IKRKFVVATTDSRHGLKVYPNRAAEMTLTGVDQLWVADITYIRLEEEFVYLAVILDAFSRRVIGWHLGETLEVSLTLNAL
RMALGQRSVSPGLVHHSDRGVQYASHDYTQLLQDNGIEISMSRKANPWDNAACESFMKTLKYEEVHRTEYRNLAHARASI
KTFLEKIYNQKRLHSSLSYRSPVEFEHSLAATASQQQVAV

Sequences:

>Translated_280_residues
MSVVQMCESLGISRAGYYRFLDPEKPAPADMDLRDEMHRVALDWPCYGSRRIVEELKARGWEVNRKRIQRLMREDNLLCV
IKRKFVVATTDSRHGLKVYPNRAAEMTLTGVDQLWVADITYIRLEEEFVYLAVILDAFSRRVIGWHLGETLEVSLTLNAL
RMALGQRSVSPGLVHHSDRGVQYASHDYTQLLQDNGIEISMSRKANPWDNAACESFMKTLKYEEVHRTEYRNLAHARASI
KTFLEKIYNQKRLHSSLSYRSPVEFEHSLAATASQQQVAV
>Mature_279_residues
SVVQMCESLGISRAGYYRFLDPEKPAPADMDLRDEMHRVALDWPCYGSRRIVEELKARGWEVNRKRIQRLMREDNLLCVI
KRKFVVATTDSRHGLKVYPNRAAEMTLTGVDQLWVADITYIRLEEEFVYLAVILDAFSRRVIGWHLGETLEVSLTLNALR
MALGQRSVSPGLVHHSDRGVQYASHDYTQLLQDNGIEISMSRKANPWDNAACESFMKTLKYEEVHRTEYRNLAHARASIK
TFLEKIYNQKRLHSSLSYRSPVEFEHSLAATASQQQVAV

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=239, Percent_Identity=33.0543933054393, Blast_Score=111, Evalue=6e-25,
Organism=Escherichia coli, GI1788405, Length=273, Percent_Identity=33.6996336996337, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1787263, Length=273, Percent_Identity=33.6996336996337, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1786753, Length=273, Percent_Identity=33.6996336996337, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1786570, Length=273, Percent_Identity=33.6996336996337, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1786490, Length=273, Percent_Identity=33.6996336996337, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1789981, Length=222, Percent_Identity=31.981981981982, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790724, Length=248, Percent_Identity=25.4032258064516, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789424, Length=248, Percent_Identity=25.4032258064516, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789224, Length=248, Percent_Identity=25.4032258064516, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788305, Length=248, Percent_Identity=25.4032258064516, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787669, Length=248, Percent_Identity=25.4032258064516, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1786558, Length=248, Percent_Identity=25.4032258064516, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32290; Mature: 32159

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVQMCESLGISRAGYYRFLDPEKPAPADMDLRDEMHRVALDWPCYGSRRIVEELKARG
CCHHHHHHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHEEECCCCCCCHHHHHHHHHCC
WEVNRKRIQRLMREDNLLCVIKRKFVVATTDSRHGLKVYPNRAAEMTLTGVDQLWVADIT
CCCCHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCEEECCCCCCEEEECCCHHHHHHHEE
YIRLEEEFVYLAVILDAFSRRVIGWHLGETLEVSLTLNALRMALGQRSVSPGLVHHSDRG
EEEEHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEHHHHHHHHHHCCCCCCCCEECCCCC
VQYASHDYTQLLQDNGIEISMSRKANPWDNAACESFMKTLKYEEVHRTEYRNLAHARASI
CEECCHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTFLEKIYNQKRLHSSLSYRSPVEFEHSLAATASQQQVAV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SVVQMCESLGISRAGYYRFLDPEKPAPADMDLRDEMHRVALDWPCYGSRRIVEELKARG
CHHHHHHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHEEECCCCCCCHHHHHHHHHCC
WEVNRKRIQRLMREDNLLCVIKRKFVVATTDSRHGLKVYPNRAAEMTLTGVDQLWVADIT
CCCCHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCEEECCCCCCEEEECCCHHHHHHHEE
YIRLEEEFVYLAVILDAFSRRVIGWHLGETLEVSLTLNALRMALGQRSVSPGLVHHSDRG
EEEEHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEHHHHHHHHHHCCCCCCCCEECCCCC
VQYASHDYTQLLQDNGIEISMSRKANPWDNAACESFMKTLKYEEVHRTEYRNLAHARASI
CEECCHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTFLEKIYNQKRLHSSLSYRSPVEFEHSLAATASQQQVAV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]