Click here to switch to the map view.

The map label for this gene is mdlA [H]

Identifier: 116622344

GI number: 116622344

Start: 4099238

End: 4100308

Strand: Reverse

Name: mdlA [H]

Synonym: Acid_3238

Alternate gene names: 116622344

Gene position: 4100308-4099238 (Counterclockwise)

Preceding gene: 116622345

Following gene: 116622341

Centisome position: 41.14

GC content: 67.23

Gene sequence:

>1071_bases
ATGCCGAAAATAACGGGGGTGCGGGTGCGGGCGGTGCGCGTTCCCATGCAGCCCCACCTGACAGCCAGCGGCACGGTGGC
GGAGTCGCCGCTGGTGCTCACGGATGTGCTCACCGATGAAGGCGTGGAAGGCCACAGCATCACGTTCACCTACACCGCCG
CGGCGCTGAAGCCGACTGGAGGGCTGATCGCGAACCTGGCGCCGCTGCTGAAGGGTGAGGAGCTTGCGCCCGCCGAACTG
GCCCGCAAACTGGCGGGCCGGTACCGGCTGCTGGGCATGCAGGGGCTGGTCGGGATGGCGCTGGCCGGACTCGACATGGC
CCTGTGGGACGCGCTGGCGCGGGTCCACAAGCTATCCCTGGCGGGGCTGCTGGGCGGGGTTCCGAAGCCGATTCGCGCGT
ATGGCGCGGTGGGGTACGATGGCGCGGCAAACTGCGCGGCGGGCGCCGAGGAGTGGGCGAAGCGCGGGTTCACCGGCGTA
AAGGCAAAGATCGGCTATCCGACGGCGGCGGAGGACCTCGAAGTGATCCGCGGGATGCGCAGGGCGTGCGGAAAAGGGAT
GTCGCTCATGGTGGACTATAACCAATCGCTCACTCCGGCGGAAGCAGTGGAGCGGCTGCGCGGGGTGGATGGCGAAGGGC
TGACGTGGATCGAGGAGCCGACGCTGGCGCACGATTACGAGGGCCACGCACAGATCGCGCGCGAGATTCGGACACCCATT
CAGTGCGGGGAAAACTGGTGGGGCGCGCTCGATATGCGCCACGCGATCGCGGCAGGGGCGAGCGATTACATGATGCCCGA
TGTGATGAAGATCGGCGGGGTCACGGGGTGGCTGCGCGCGGCGGCTTTGGGAGAGGCCCACAGCATCCGGCTCTCAAATC
ATCTGTGGCCTGAAATCAGCGCCCAGTTACTGAGCGTCACGCCCACGGCGCACTGGTTGGAGTACGCGGACTGGTGGAAC
CCGATTCTGGCCGAGCCGCTGCGAGTCGAGAATGGAATGGCGATTTGCGACGGCGTCACCGGCACCGGCGTGGAGTGGAA
CCAGACGGCGGTCGAGAAGTACCTGGCCTAG

Upstream 100 bases:

>100_bases
GTTCGCGCGTACAACGGACGCTCCCTTACGGTCGCGGCTTTGATGCACAGGAGTTCGCGGGGCCAGGAAACAAAAGCGGT
CTAATACCCGGAGGAAGCAG

Downstream 100 bases:

>100_bases
AACGGGCCCGCGAGATATCCGAGCAAGGTAAGCAGGATCAACGCACCACCCAAAATGGCTAGCCCCATGGGCGGCTGGAT
TGGTTCATGGCGGCGGGCGT

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: MR [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MPKITGVRVRAVRVPMQPHLTASGTVAESPLVLTDVLTDEGVEGHSITFTYTAAALKPTGGLIANLAPLLKGEELAPAEL
ARKLAGRYRLLGMQGLVGMALAGLDMALWDALARVHKLSLAGLLGGVPKPIRAYGAVGYDGAANCAAGAEEWAKRGFTGV
KAKIGYPTAAEDLEVIRGMRRACGKGMSLMVDYNQSLTPAEAVERLRGVDGEGLTWIEEPTLAHDYEGHAQIAREIRTPI
QCGENWWGALDMRHAIAAGASDYMMPDVMKIGGVTGWLRAAALGEAHSIRLSNHLWPEISAQLLSVTPTAHWLEYADWWN
PILAEPLRVENGMAICDGVTGTGVEWNQTAVEKYLA

Sequences:

>Translated_356_residues
MPKITGVRVRAVRVPMQPHLTASGTVAESPLVLTDVLTDEGVEGHSITFTYTAAALKPTGGLIANLAPLLKGEELAPAEL
ARKLAGRYRLLGMQGLVGMALAGLDMALWDALARVHKLSLAGLLGGVPKPIRAYGAVGYDGAANCAAGAEEWAKRGFTGV
KAKIGYPTAAEDLEVIRGMRRACGKGMSLMVDYNQSLTPAEAVERLRGVDGEGLTWIEEPTLAHDYEGHAQIAREIRTPI
QCGENWWGALDMRHAIAAGASDYMMPDVMKIGGVTGWLRAAALGEAHSIRLSNHLWPEISAQLLSVTPTAHWLEYADWWN
PILAEPLRVENGMAICDGVTGTGVEWNQTAVEKYLA
>Mature_355_residues
PKITGVRVRAVRVPMQPHLTASGTVAESPLVLTDVLTDEGVEGHSITFTYTAAALKPTGGLIANLAPLLKGEELAPAELA
RKLAGRYRLLGMQGLVGMALAGLDMALWDALARVHKLSLAGLLGGVPKPIRAYGAVGYDGAANCAAGAEEWAKRGFTGVK
AKIGYPTAAEDLEVIRGMRRACGKGMSLMVDYNQSLTPAEAVERLRGVDGEGLTWIEEPTLAHDYEGHAQIAREIRTPIQ
CGENWWGALDMRHAIAAGASDYMMPDVMKIGGVTGWLRAAALGEAHSIRLSNHLWPEISAQLLSVTPTAHWLEYADWWNP
ILAEPLRVENGMAICDGVTGTGVEWNQTAVEKYLA

Specific function: D-galactonate degradation; first step. D-galactonate degradation; third step. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=204, Percent_Identity=26.4705882352941, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787864, Length=303, Percent_Identity=22.7722772277228, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.1.2.2 [H]

Molecular weight: Translated: 38108; Mature: 37977

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKITGVRVRAVRVPMQPHLTASGTVAESPLVLTDVLTDEGVEGHSITFTYTAAALKPTG
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEHHHCCCCCCCEEEEEEEEEEECCCC
GLIANLAPLLKGEELAPAELARKLAGRYRLLGMQGLVGMALAGLDMALWDALARVHKLSL
CHHHHHHHHHCCCCCCHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLLGGVPKPIRAYGAVGYDGAANCAAGAEEWAKRGFTGVKAKIGYPTAAEDLEVIRGMR
HHHHCCCCHHHHHHCCCCCCCCCHHHCCHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHH
RACGKGMSLMVDYNQSLTPAEAVERLRGVDGEGLTWIEEPTLAHDYEGHAQIAREIRTPI
HHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHCHH
QCGENWWGALDMRHAIAAGASDYMMPDVMKIGGVTGWLRAAALGEAHSIRLSNHLWPEIS
HCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHCCCHHEEEECCCCCHHHH
AQLLSVTPTAHWLEYADWWNPILAEPLRVENGMAICDGVTGTGVEWNQTAVEKYLA
HHHHCCCCHHHHHHHHHHCCHHHCCCEEECCCCEEECCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
PKITGVRVRAVRVPMQPHLTASGTVAESPLVLTDVLTDEGVEGHSITFTYTAAALKPTG
CCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEHHHCCCCCCCEEEEEEEEEEECCCC
GLIANLAPLLKGEELAPAELARKLAGRYRLLGMQGLVGMALAGLDMALWDALARVHKLSL
CHHHHHHHHHCCCCCCHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLLGGVPKPIRAYGAVGYDGAANCAAGAEEWAKRGFTGVKAKIGYPTAAEDLEVIRGMR
HHHHCCCCHHHHHHCCCCCCCCCHHHCCHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHH
RACGKGMSLMVDYNQSLTPAEAVERLRGVDGEGLTWIEEPTLAHDYEGHAQIAREIRTPI
HHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHCHH
QCGENWWGALDMRHAIAAGASDYMMPDVMKIGGVTGWLRAAALGEAHSIRLSNHLWPEIS
HCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHCCCHHEEEECCCCCHHHH
AQLLSVTPTAHWLEYADWWNPILAEPLRVENGMAICDGVTGTGVEWNQTAVEKYLA
HHHHCCCCHHHHHHHHHHCCHHHCCCEEECCCCEEECCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2831968; 2271624; 2215699; 1909893; 1892834; 7893689; 7893690 [H]