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The map label for this gene is zraS [H]

Identifier: 116622221

GI number: 116622221

Start: 3948440

End: 3949129

Strand: Direct

Name: zraS [H]

Synonym: Acid_3114

Alternate gene names: 116622221

Gene position: 3948440-3949129 (Clockwise)

Preceding gene: 116622216

Following gene: 116622223

Centisome position: 39.62

GC content: 61.88

Gene sequence:

>690_bases
ATGAACACAACCTCCAATCAAAGCTCGTTAACTGAAGTACAGTCATTTCTGCATGATTTGCGCAACCCCCTGTCGGCCAT
TCACGGTGGCGCGGAGATACTGGTGCGGTCTAACCTTTCGCAGCCGCAGGTTCACCGCATCGCGCGCAACATGTGCTGCG
CCGCTATCCGAATTCGCGAATTGCTGGAACTCTGCCTGGATCGCAGCCGCTGCCGGACCAAAAAAGTGGAAATCGCCGAT
CTGCGCGCGGTGGTGGCCGCCGCGGTGGACCGGATTGCAGTCAGTGCCGAGTTTCAAGCCGTACAGATCGTGCAGGCCAT
ACCGGAACGACTCCGCATTGCGATGGACCTCAATCGCATCCATCGCGCCCTCGTGAATCTTCTGGTCAACGCGTTGGAAG
TCATGCCACAAGGTGGAACGATCCACGTATCCGCGATTCCCGAGGGCCGCTCGGTATTGATCCGCGTTCGCGATACCGGC
CCGGGGATCGCTCCGGAAATCAGGGATCGCCTCTTCCAACCCTTCGCCACCGCGGGCAAGGCGGGCGGTCTCGGTCTCGG
CCTGGCCTTCTCACGGCAGGCTGCGATCGATCACGGCGGCGAGATCTGGGCGGAATCGCCTAGCCGCGGAGCCTGCCTCG
TCATTCGACTTCCGCGCACGCTCGCCAAGCCGCGCGCAGCCTCACGCTGA

Upstream 100 bases:

>100_bases
GAAAGGAGTCGCATTTCCGTTCCGAGGATGAACTCATTGCATTCCTGCGCGAACGCTTTGCCGAGAGTTTTCAGCGGGCG
TCGCGAAATGAAGGGACAGT

Downstream 100 bases:

>100_bases
GGCCACCCGCGGGAGAAGCCGCCGGACCGGTGGAGCGTTGAGCAGCGACTCGATCAGTGGATCGCATTCCTCAAACGAGC
CGGCCAGCTGCCGGATAATT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MNTTSNQSSLTEVQSFLHDLRNPLSAIHGGAEILVRSNLSQPQVHRIARNMCCAAIRIRELLELCLDRSRCRTKKVEIAD
LRAVVAAAVDRIAVSAEFQAVQIVQAIPERLRIAMDLNRIHRALVNLLVNALEVMPQGGTIHVSAIPEGRSVLIRVRDTG
PGIAPEIRDRLFQPFATAGKAGGLGLGLAFSRQAAIDHGGEIWAESPSRGACLVIRLPRTLAKPRAASR

Sequences:

>Translated_229_residues
MNTTSNQSSLTEVQSFLHDLRNPLSAIHGGAEILVRSNLSQPQVHRIARNMCCAAIRIRELLELCLDRSRCRTKKVEIAD
LRAVVAAAVDRIAVSAEFQAVQIVQAIPERLRIAMDLNRIHRALVNLLVNALEVMPQGGTIHVSAIPEGRSVLIRVRDTG
PGIAPEIRDRLFQPFATAGKAGGLGLGLAFSRQAAIDHGGEIWAESPSRGACLVIRLPRTLAKPRAASR
>Mature_229_residues
MNTTSNQSSLTEVQSFLHDLRNPLSAIHGGAEILVRSNLSQPQVHRIARNMCCAAIRIRELLELCLDRSRCRTKKVEIAD
LRAVVAAAVDRIAVSAEFQAVQIVQAIPERLRIAMDLNRIHRALVNLLVNALEVMPQGGTIHVSAIPEGRSVLIRVRDTG
PGIAPEIRDRLFQPFATAGKAGGLGLGLAFSRQAAIDHGGEIWAESPSRGACLVIRLPRTLAKPRAASR

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=206, Percent_Identity=30.5825242718447, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1790861, Length=214, Percent_Identity=31.3084112149533, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1786912, Length=215, Percent_Identity=33.0232558139535, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI87081816, Length=211, Percent_Identity=30.8056872037915, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788549, Length=205, Percent_Identity=30.2439024390244, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1790300, Length=219, Percent_Identity=27.8538812785388, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789808, Length=199, Percent_Identity=30.6532663316583, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790346, Length=225, Percent_Identity=30.2222222222222, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1786783, Length=210, Percent_Identity=27.1428571428571, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI48994928, Length=214, Percent_Identity=25.2336448598131, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787894, Length=201, Percent_Identity=29.8507462686567, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786600, Length=191, Percent_Identity=27.2251308900524, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 24877; Mature: 24877

Theoretical pI: Translated: 11.18; Mature: 11.18

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTSNQSSLTEVQSFLHDLRNPLSAIHGGAEILVRSNLSQPQVHRIARNMCCAAIRIRE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHH
LLELCLDRSRCRTKKVEIADLRAVVAAAVDRIAVSAEFQAVQIVQAIPERLRIAMDLNRI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HRALVNLLVNALEVMPQGGTIHVSAIPEGRSVLIRVRDTGPGIAPEIRDRLFQPFATAGK
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCC
AGGLGLGLAFSRQAAIDHGGEIWAESPSRGACLVIRLPRTLAKPRAASR
CCCCEECHHHHHHHHHCCCCCEECCCCCCCCEEEEECCHHHCCCCCCCC
>Mature Secondary Structure
MNTTSNQSSLTEVQSFLHDLRNPLSAIHGGAEILVRSNLSQPQVHRIARNMCCAAIRIRE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHH
LLELCLDRSRCRTKKVEIADLRAVVAAAVDRIAVSAEFQAVQIVQAIPERLRIAMDLNRI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HRALVNLLVNALEVMPQGGTIHVSAIPEGRSVLIRVRDTGPGIAPEIRDRLFQPFATAGK
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCC
AGGLGLGLAFSRQAAIDHGGEIWAESPSRGACLVIRLPRTLAKPRAASR
CCCCEECHHHHHHHHHCCCCCEECCCCCCCCEEEEECCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]