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The map label for this gene is mls [H]

Identifier: 116622215

GI number: 116622215

Start: 3937415

End: 3939016

Strand: Direct

Name: mls [H]

Synonym: Acid_3107

Alternate gene names: 116622215

Gene position: 3937415-3939016 (Clockwise)

Preceding gene: 116622214

Following gene: 116622216

Centisome position: 39.51

GC content: 61.8

Gene sequence:

>1602_bases
ATGAGCAGTGGAACCATGCAGGCGCCAAAGGGCACGGAAATTCAGGGGCTTGTGGAAGGCGAACACGCCGGGATCCTGAC
CAGGGAAGCAATCGAGTTCATCGCCGGTCTCTCGCGGCATTTCGAACCCCGGCGGCGTGAATTGCTCGCCCGCAGAGCGG
AACGCCAGTTGGCGCTCGACGCAGGCGCGCGACCGGATTTTCTTCCGGAAACGGCGGAGATTCGGGCCGGCGAGTGGCGG
GTCGCGCCGATTCCGAAGGATCTCGAAGACCGGCGCGTCGAGATCACCGGGCCCGTCGATCGCAAGATGATCATCAATGC
GCTCAACTCCGGCGCCAATGTGTTCATGGCGGATTTCGAGGATTCCAACTCGCCCACCTGGCGCAACAACCTGGATGGGC
AGGTGAACCTGCGCGACGCCGTCCGCGGCACAATCGCGTTCGAGAGCGGGGAAGGGAAGCAGTACCGGCTTAATCCCCGT
CCCGCAGTCCTGTTCGTGCGGCCGCGAGGCTGGCATTTGACGGAGCGGCACTTTTTGGTGGACGGCGAGCCGATCTCCGG
TTCGCTTTTCGATTTCGGCCTCTACTTCTTTCACAACGCGCGGGCACTGATGGAAAAGGGATCGGGACCATATTTTTATC
TGCCCAAGATGGAAAGCCATCTCGAAGCGCGCTTGTGGAACGATGTCTTCACCGCGGCTCAGGATGCCTTGCGCATTCCG
CGCGGCACCATTCGAGCTACCGTGTTGATCGAGACTATACTCGCTGCATTCGAGATGGACGAAATCCTCTGGGAACTGCG
CGATCATTCCGCCGGGCTGAACTGCGGGCGGTGGGACTACATTTTCAGCTTCATTAAGAAGTTCCGCAACCGGGAAGGTT
TCGTGCTGCCCAATCGAGCAGTGGTGACGATGGACCGGCATTTTCTGGATTCCTACGTCCGCCTGCTGATTGAGACGTGC
CACCGCCGCGGTATCCACGCGATGGGCGGTATGGCGGCGCAGGTACCGATCAAAGATGATCGGGCGGCCAATGAGCAGGC
CATCGAAAAGGTCCGCCGCGACAAGATCCGCGAGGTGCGCGCGGGACATGACGGAACCTGGGTGGCGCATCCGGGACTGG
TACCGGTCGCGAAACAGGTCTTCGACGAAGTCTTGAAAGAGCCGAATCAGATCGCCATCAAGCGCGACGACGCGCGCGTC
GTCGCCGTCGATCTGCTCGCCATTCCCGACGGTGAAATTACCGAACAGGGCGTGCGGTGGAATATCGACGTGGGCCTGCG
CTATCTGGAGGCGTGGCTGCGCGGCTCCGGTTGCGTGCCGATCTACAACCTGATGGAAGACGCGGCCACCGCCGAGATCT
GCCGGGCCCAGATCTGGCAGTGGGTTCGTCACGGCGCCGCTTTGCAGGATGGCCGAAAGGTCACGGCGGCCTGGGTGCTG
CAGGCGTGCGCGGAGCAGTTACAGAAGATTCGCGAGGATCTGGGGGACGCCGCCTTCTCGCAGGGCCGCTTCGCGGAGGC
TGCCCGCATTTTCGAGCCGATGGTGACGGGATCTGAATTTCCGGAGTTCCTGACGCTGGCGGCTTACGCGTCTATCGATT
GA

Upstream 100 bases:

>100_bases
CGATGCGGCCGGGAAGAAGTGGGTGCGCTTCGCGGTGTGGGATTCAGCGGGCAACGGCGCGTTCACGCAGCCGGTGCATC
TCAAGTGAAGGAGAGATGAA

Downstream 100 bases:

>100_bases
CTGGCGCTCTGGGAACAAACCATCGTCCGCCTCGTTACTGTTGACGTAAGGAGTGCTGTGCGGTCAATCTGGCGAGATCT
CCGCTACGGCTATCGGACCC

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MSSGTMQAPKGTEIQGLVEGEHAGILTREAIEFIAGLSRHFEPRRRELLARRAERQLALDAGARPDFLPETAEIRAGEWR
VAPIPKDLEDRRVEITGPVDRKMIINALNSGANVFMADFEDSNSPTWRNNLDGQVNLRDAVRGTIAFESGEGKQYRLNPR
PAVLFVRPRGWHLTERHFLVDGEPISGSLFDFGLYFFHNARALMEKGSGPYFYLPKMESHLEARLWNDVFTAAQDALRIP
RGTIRATVLIETILAAFEMDEILWELRDHSAGLNCGRWDYIFSFIKKFRNREGFVLPNRAVVTMDRHFLDSYVRLLIETC
HRRGIHAMGGMAAQVPIKDDRAANEQAIEKVRRDKIREVRAGHDGTWVAHPGLVPVAKQVFDEVLKEPNQIAIKRDDARV
VAVDLLAIPDGEITEQGVRWNIDVGLRYLEAWLRGSGCVPIYNLMEDAATAEICRAQIWQWVRHGAALQDGRKVTAAWVL
QACAEQLQKIREDLGDAAFSQGRFAEAARIFEPMVTGSEFPEFLTLAAYASID

Sequences:

>Translated_533_residues
MSSGTMQAPKGTEIQGLVEGEHAGILTREAIEFIAGLSRHFEPRRRELLARRAERQLALDAGARPDFLPETAEIRAGEWR
VAPIPKDLEDRRVEITGPVDRKMIINALNSGANVFMADFEDSNSPTWRNNLDGQVNLRDAVRGTIAFESGEGKQYRLNPR
PAVLFVRPRGWHLTERHFLVDGEPISGSLFDFGLYFFHNARALMEKGSGPYFYLPKMESHLEARLWNDVFTAAQDALRIP
RGTIRATVLIETILAAFEMDEILWELRDHSAGLNCGRWDYIFSFIKKFRNREGFVLPNRAVVTMDRHFLDSYVRLLIETC
HRRGIHAMGGMAAQVPIKDDRAANEQAIEKVRRDKIREVRAGHDGTWVAHPGLVPVAKQVFDEVLKEPNQIAIKRDDARV
VAVDLLAIPDGEITEQGVRWNIDVGLRYLEAWLRGSGCVPIYNLMEDAATAEICRAQIWQWVRHGAALQDGRKVTAAWVL
QACAEQLQKIREDLGDAAFSQGRFAEAARIFEPMVTGSEFPEFLTLAAYASID
>Mature_532_residues
SSGTMQAPKGTEIQGLVEGEHAGILTREAIEFIAGLSRHFEPRRRELLARRAERQLALDAGARPDFLPETAEIRAGEWRV
APIPKDLEDRRVEITGPVDRKMIINALNSGANVFMADFEDSNSPTWRNNLDGQVNLRDAVRGTIAFESGEGKQYRLNPRP
AVLFVRPRGWHLTERHFLVDGEPISGSLFDFGLYFFHNARALMEKGSGPYFYLPKMESHLEARLWNDVFTAAQDALRIPR
GTIRATVLIETILAAFEMDEILWELRDHSAGLNCGRWDYIFSFIKKFRNREGFVLPNRAVVTMDRHFLDSYVRLLIETCH
RRGIHAMGGMAAQVPIKDDRAANEQAIEKVRRDKIREVRAGHDGTWVAHPGLVPVAKQVFDEVLKEPNQIAIKRDDARVV
AVDLLAIPDGEITEQGVRWNIDVGLRYLEAWLRGSGCVPIYNLMEDAATAEICRAQIWQWVRHGAALQDGRKVTAAWVLQ
ACAEQLQKIREDLGDAAFSQGRFAEAARIFEPMVTGSEFPEFLTLAAYASID

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=507, Percent_Identity=56.8047337278106, Blast_Score=591, Evalue=1e-170,
Organism=Caenorhabditis elegans, GI17561814, Length=531, Percent_Identity=54.6139359698682, Blast_Score=578, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI71982926, Length=428, Percent_Identity=55.607476635514, Blast_Score=480, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6324212, Length=518, Percent_Identity=56.5637065637066, Blast_Score=585, Evalue=1e-168,
Organism=Saccharomyces cerevisiae, GI6322222, Length=520, Percent_Identity=55.1923076923077, Blast_Score=572, Evalue=1e-164,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 60151; Mature: 60020

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGTMQAPKGTEIQGLVEGEHAGILTREAIEFIAGLSRHFEPRRRELLARRAERQLALD
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
AGARPDFLPETAEIRAGEWRVAPIPKDLEDRRVEITGPVDRKMIINALNSGANVFMADFE
CCCCCCCCCCCHHCCCCCEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECC
DSNSPTWRNNLDGQVNLRDAVRGTIAFESGEGKQYRLNPRPAVLFVRPRGWHLTERHFLV
CCCCCCCCCCCCCCEEHHHHHCEEEEEECCCCCEEEECCCCEEEEECCCCCCEECCEEEE
DGEPISGSLFDFGLYFFHNARALMEKGSGPYFYLPKMESHLEARLWNDVFTAAQDALRIP
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
RGTIRATVLIETILAAFEMDEILWELRDHSAGLNCGRWDYIFSFIKKFRNREGFVLPNRA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCE
VVTMDRHFLDSYVRLLIETCHRRGIHAMGGMAAQVPIKDDRAANEQAIEKVRRDKIREVR
EEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHH
AGHDGTWVAHPGLVPVAKQVFDEVLKEPNQIAIKRDDARVVAVDLLAIPDGEITEQGVRW
CCCCCCEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCCEE
NIDVGLRYLEAWLRGSGCVPIYNLMEDAATAEICRAQIWQWVRHGAALQDGRKVTAAWVL
EHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHH
QACAEQLQKIREDLGDAAFSQGRFAEAARIFEPMVTGSEFPEFLTLAAYASID
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SSGTMQAPKGTEIQGLVEGEHAGILTREAIEFIAGLSRHFEPRRRELLARRAERQLALD
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
AGARPDFLPETAEIRAGEWRVAPIPKDLEDRRVEITGPVDRKMIINALNSGANVFMADFE
CCCCCCCCCCCHHCCCCCEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECC
DSNSPTWRNNLDGQVNLRDAVRGTIAFESGEGKQYRLNPRPAVLFVRPRGWHLTERHFLV
CCCCCCCCCCCCCCEEHHHHHCEEEEEECCCCCEEEECCCCEEEEECCCCCCEECCEEEE
DGEPISGSLFDFGLYFFHNARALMEKGSGPYFYLPKMESHLEARLWNDVFTAAQDALRIP
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
RGTIRATVLIETILAAFEMDEILWELRDHSAGLNCGRWDYIFSFIKKFRNREGFVLPNRA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCE
VVTMDRHFLDSYVRLLIETCHRRGIHAMGGMAAQVPIKDDRAANEQAIEKVRRDKIREVR
EEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHH
AGHDGTWVAHPGLVPVAKQVFDEVLKEPNQIAIKRDDARVVAVDLLAIPDGEITEQGVRW
CCCCCCEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCCEE
NIDVGLRYLEAWLRGSGCVPIYNLMEDAATAEICRAQIWQWVRHGAALQDGRKVTAAWVL
EHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHH
QACAEQLQKIREDLGDAAFSQGRFAEAARIFEPMVTGSEFPEFLTLAAYASID
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA