Click here to switch to the map view.

The map label for this gene is ykoU [H]

Identifier: 116622141

GI number: 116622141

Start: 3832244

End: 3834175

Strand: Direct

Name: ykoU [H]

Synonym: Acid_3033

Alternate gene names: 116622141

Gene position: 3832244-3834175 (Clockwise)

Preceding gene: 116622140

Following gene: 116622144

Centisome position: 38.45

GC content: 54.97

Gene sequence:

>1932_bases
GTGGTCATTGCCAGGAAAACGAAACCGAGAGGCGTTCCCACCGAACCTGTGCCTGGACCAATTCGTTCCTCCACGCACAA
AATTGGCGAGCTCATGGCTCGATTCCCCGAAGTCCAACTGGCAACACTTGTTGCTGCGGTCCCCACGGGTGAAAATTGGG
TTCAGGAAATTAAGTTCGATGGCTATCGACTTCTCGGATTTCGTTGGCAACAATCCGTTAGGCTGATTACGCGTAACGGG
AAGGATTGGACTGAAAGCTTTCCTTCCCTCTCAGCTGCGCTTTCAGGATTAGCCGTGGACAGCGCAGTGTTGGACATGGA
AGCGGTGGTCCTCGATGGCAGGGGTAAAAGCAGTTTCCAAGCTCTGCAGAATGCGCTGGGAGAGGGCGGGGATCGCCGGA
GGATCGTCGCATATGTCTTCGATCTTCTGTATCTCAACGGAAGAGATTTAACCAAACTGACACTGCTGGAGCGCAAGGAG
CAGCTCGAAACGATTCTTGGAACATTCAAAATTCTCCGTTACAGTAACCATGTCATCGGACAAGGCGAAGAGATGCTGGC
GAAGGCCTGTGAGGCCGGACTGGAAGGCATCATTTCGAAGGAGGCCAACGCCGCGTACTTGCCGGGCCGCAGCAGGAACT
GGCTCAAAATCAAGTGTTCCTTGCGCCAGGAGTTTGTCATCGTGGGATACAGCGATCCGCGAAAAGGCGGGCGAGCCCTG
GGTGCTCTCTACCTCGGCTATCGCAAGAATGGTGCTCTTCAGTATGCGGGGAAGGTCGGCACCGGGTTTACGATGAAGAG
CGCCCGGGAACTGACTGATCGCCTGAGCAGGATTTCGGTACATGCGCCTGTGCTAAGCCGCACCCAGGCAGCGGGACTTG
GCGCTGGTGAATGGCGCGCGGTCCATTGGGTGAAGCCGATCATGCTGTGCGAAGTCGCGTTTACCGAATGGACTGGAGAC
GGCCGCATTCGGCACCCTTCTTTTCAAGGTTTACGTGAAGACAAGAGTGCCTCTGAGGTCAAGAAGGAGATGCCCGTGGC
GCCTAAGGACAAAACGAAAGCGATAGAAGGCAAGAAAGTCCTGCACGGAATTACGATTACTCACCCCGAACGCGTGATCT
CCGATACCGGTCAGATCACGAAGGGTGAACTTGCCGAATATTATGCGGCGGTCGCCCCGTGGATGCTCCCGCACATCGCC
CGCCATCCGCTAAGCCTCCTGCGTTGCCCCGCAGGGATCGATAAGCAATGTTTCTTCCAGAGAAACCCAGGCAAAGGTCT
GGGCGCCGATGTTCACCCCTTCGAATTCAAAAACAAAGGGAAGACGTACGAATATCTGTATATCGAGGATGAAGCAGGAT
TGCTTGAAGTGATACAAATGGGCGCTGTCGAACTCCACCCGTGGGGCGCTTCGATCGATTCGATCGATTATCCGGATCGC
ATGATCTTCGATCTTGACCCCGCGCCCGAAGTGCCGTTCGAAGCGTTGAAACTCGCCGCGCAGGATCTTCGCAAGCGCCT
CCAGCGTAACGGCCTGGAATCATCACTAAAATGCACGGGAGGAAAGGGTCTCCATGTCACCGTTCCATTAGCTGCGACGG
ATTCCTGGTCGATGGTAAAACGCTTTGCCGCTTCGATCGCGGACGAGATGGTAGCCGCAACGCCCGAGGCATATGTCGCG
ACGATGAGCAAAGCCAAAAGGACGGGTAGGATTTTCATCGACTATTTCCGGAATGATTACACTGCGACTGCGATCGCCGA
CTATTCGGTTCGAGCGCGTCCTGGAGCGCCTGTTGCCTTGCCCATCGACTGGAAGGAACTGAAGAATTTGGAATCGGCCA
GTCAATTCACGATGAAAGATGTTCTGTCACGGTTGAGGAGAAAGAAAAAGACACCCGCCGCGCTTTTCCGCTATACAATC
CCCGCGGGTTAG

Upstream 100 bases:

>100_bases
GAATCAACACCTGCATACGCTTTACTCGCGTCTCCTCAGTGTTCAAGCTATATTACCAGGGTAGTTCTGCCTATCATCGT
CGGCTACAGCGGAGGCTATG

Downstream 100 bases:

>100_bases
GCCCTGCGTCTACTCAGGTTCAATCGGATGGCGAAACAGTGTTCCATTGTGTACTACAATTTGGCAAGCCGTTCGAAAGG
CGTCTCCGCTAATCGAAAAG

Product: ATP dependent DNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MVIARKTKPRGVPTEPVPGPIRSSTHKIGELMARFPEVQLATLVAAVPTGENWVQEIKFDGYRLLGFRWQQSVRLITRNG
KDWTESFPSLSAALSGLAVDSAVLDMEAVVLDGRGKSSFQALQNALGEGGDRRRIVAYVFDLLYLNGRDLTKLTLLERKE
QLETILGTFKILRYSNHVIGQGEEMLAKACEAGLEGIISKEANAAYLPGRSRNWLKIKCSLRQEFVIVGYSDPRKGGRAL
GALYLGYRKNGALQYAGKVGTGFTMKSARELTDRLSRISVHAPVLSRTQAAGLGAGEWRAVHWVKPIMLCEVAFTEWTGD
GRIRHPSFQGLREDKSASEVKKEMPVAPKDKTKAIEGKKVLHGITITHPERVISDTGQITKGELAEYYAAVAPWMLPHIA
RHPLSLLRCPAGIDKQCFFQRNPGKGLGADVHPFEFKNKGKTYEYLYIEDEAGLLEVIQMGAVELHPWGASIDSIDYPDR
MIFDLDPAPEVPFEALKLAAQDLRKRLQRNGLESSLKCTGGKGLHVTVPLAATDSWSMVKRFAASIADEMVAATPEAYVA
TMSKAKRTGRIFIDYFRNDYTATAIADYSVRARPGAPVALPIDWKELKNLESASQFTMKDVLSRLRRKKKTPAALFRYTI
PAG

Sequences:

>Translated_643_residues
MVIARKTKPRGVPTEPVPGPIRSSTHKIGELMARFPEVQLATLVAAVPTGENWVQEIKFDGYRLLGFRWQQSVRLITRNG
KDWTESFPSLSAALSGLAVDSAVLDMEAVVLDGRGKSSFQALQNALGEGGDRRRIVAYVFDLLYLNGRDLTKLTLLERKE
QLETILGTFKILRYSNHVIGQGEEMLAKACEAGLEGIISKEANAAYLPGRSRNWLKIKCSLRQEFVIVGYSDPRKGGRAL
GALYLGYRKNGALQYAGKVGTGFTMKSARELTDRLSRISVHAPVLSRTQAAGLGAGEWRAVHWVKPIMLCEVAFTEWTGD
GRIRHPSFQGLREDKSASEVKKEMPVAPKDKTKAIEGKKVLHGITITHPERVISDTGQITKGELAEYYAAVAPWMLPHIA
RHPLSLLRCPAGIDKQCFFQRNPGKGLGADVHPFEFKNKGKTYEYLYIEDEAGLLEVIQMGAVELHPWGASIDSIDYPDR
MIFDLDPAPEVPFEALKLAAQDLRKRLQRNGLESSLKCTGGKGLHVTVPLAATDSWSMVKRFAASIADEMVAATPEAYVA
TMSKAKRTGRIFIDYFRNDYTATAIADYSVRARPGAPVALPIDWKELKNLESASQFTMKDVLSRLRRKKKTPAALFRYTI
PAG
>Mature_643_residues
MVIARKTKPRGVPTEPVPGPIRSSTHKIGELMARFPEVQLATLVAAVPTGENWVQEIKFDGYRLLGFRWQQSVRLITRNG
KDWTESFPSLSAALSGLAVDSAVLDMEAVVLDGRGKSSFQALQNALGEGGDRRRIVAYVFDLLYLNGRDLTKLTLLERKE
QLETILGTFKILRYSNHVIGQGEEMLAKACEAGLEGIISKEANAAYLPGRSRNWLKIKCSLRQEFVIVGYSDPRKGGRAL
GALYLGYRKNGALQYAGKVGTGFTMKSARELTDRLSRISVHAPVLSRTQAAGLGAGEWRAVHWVKPIMLCEVAFTEWTGD
GRIRHPSFQGLREDKSASEVKKEMPVAPKDKTKAIEGKKVLHGITITHPERVISDTGQITKGELAEYYAAVAPWMLPHIA
RHPLSLLRCPAGIDKQCFFQRNPGKGLGADVHPFEFKNKGKTYEYLYIEDEAGLLEVIQMGAVELHPWGASIDSIDYPDR
MIFDLDPAPEVPFEALKLAAQDLRKRLQRNGLESSLKCTGGKGLHVTVPLAATDSWSMVKRFAASIADEMVAATPEAYVA
TMSKAKRTGRIFIDYFRNDYTATAIADYSVRARPGAPVALPIDWKELKNLESASQFTMKDVLSRLRRKKKTPAALFRYTI
PAG

Specific function: Probably involved in the repair of DNA double-strand breaks by non-homologous-end joining (NHEJ) during spore germination [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=380, Percent_Identity=25.7894736842105, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71985269, Length=366, Percent_Identity=25.1366120218579, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320038, Length=370, Percent_Identity=24.8648648648649, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324578, Length=228, Percent_Identity=29.8245614035088, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24762424, Length=379, Percent_Identity=25.8575197889182, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR014146
- InterPro:   IPR014145
- InterPro:   IPR014143 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 71215; Mature: 71215

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIARKTKPRGVPTEPVPGPIRSSTHKIGELMARFPEVQLATLVAAVPTGENWVQEIKFD
CEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHEEHHHEECCCCHHHHHHHCCC
GYRLLGFRWQQSVRLITRNGKDWTESFPSLSAALSGLAVDSAVLDMEAVVLDGRGKSSFQ
CEEEEEEEHHCCEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHH
ALQNALGEGGDRRRIVAYVFDLLYLNGRDLTKLTLLERKEQLETILGTFKILRYSNHVIG
HHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QGEEMLAKACEAGLEGIISKEANAAYLPGRSRNWLKIKCSLRQEFVIVGYSDPRKGGRAL
CCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEEECCEEEEEEECCCCCCCCHHH
GALYLGYRKNGALQYAGKVGTGFTMKSARELTDRLSRISVHAPVLSRTQAAGLGAGEWRA
HHHEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEE
VHWVKPIMLCEVAFTEWTGDGRIRHPSFQGLREDKSASEVKKEMPVAPKDKTKAIEGKKV
HHHHHHHHHHHHHHEEECCCCCEECCCCCCCCCCCCHHHHHHHCCCCCCCCHHHCCCCHH
LHGITITHPERVISDTGQITKGELAEYYAAVAPWMLPHIARHPLSLLRCPAGIDKQCFFQ
EEEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHEEE
RNPGKGLGADVHPFEFKNKGKTYEYLYIEDEAGLLEVIQMGAVELHPWGASIDSIDYPDR
CCCCCCCCCCCCCCEECCCCCEEEEEEEECCCCHHHHHHHCCEEEECCCCCCCCCCCCCC
MIFDLDPAPEVPFEALKLAAQDLRKRLQRNGLESSLKCTGGKGLHVTVPLAATDSWSMVK
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEEEEECCCCHHHHH
RFAASIADEMVAATPEAYVATMSKAKRTGRIFIDYFRNDYTATAIADYSVRARPGAPVAL
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCEEECCCCCCEEE
PIDWKELKNLESASQFTMKDVLSRLRRKKKTPAALFRYTIPAG
CCCHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCC
>Mature Secondary Structure
MVIARKTKPRGVPTEPVPGPIRSSTHKIGELMARFPEVQLATLVAAVPTGENWVQEIKFD
CEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHEEHHHEECCCCHHHHHHHCCC
GYRLLGFRWQQSVRLITRNGKDWTESFPSLSAALSGLAVDSAVLDMEAVVLDGRGKSSFQ
CEEEEEEEHHCCEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHH
ALQNALGEGGDRRRIVAYVFDLLYLNGRDLTKLTLLERKEQLETILGTFKILRYSNHVIG
HHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QGEEMLAKACEAGLEGIISKEANAAYLPGRSRNWLKIKCSLRQEFVIVGYSDPRKGGRAL
CCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEEECCEEEEEEECCCCCCCCHHH
GALYLGYRKNGALQYAGKVGTGFTMKSARELTDRLSRISVHAPVLSRTQAAGLGAGEWRA
HHHEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEE
VHWVKPIMLCEVAFTEWTGDGRIRHPSFQGLREDKSASEVKKEMPVAPKDKTKAIEGKKV
HHHHHHHHHHHHHHEEECCCCCEECCCCCCCCCCCCHHHHHHHCCCCCCCCHHHCCCCHH
LHGITITHPERVISDTGQITKGELAEYYAAVAPWMLPHIARHPLSLLRCPAGIDKQCFFQ
EEEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHEEE
RNPGKGLGADVHPFEFKNKGKTYEYLYIEDEAGLLEVIQMGAVELHPWGASIDSIDYPDR
CCCCCCCCCCCCCCEECCCCCEEEEEEEECCCCHHHHHHHCCEEEECCCCCCCCCCCCCC
MIFDLDPAPEVPFEALKLAAQDLRKRLQRNGLESSLKCTGGKGLHVTVPLAATDSWSMVK
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEEEEECCCCHHHHH
RFAASIADEMVAATPEAYVATMSKAKRTGRIFIDYFRNDYTATAIADYSVRARPGAPVAL
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCEEECCCCCCEEE
PIDWKELKNLESASQFTMKDVLSRLRRKKKTPAALFRYTIPAG
CCCHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]