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The map label for this gene is cmoB [H]

Identifier: 116622132

GI number: 116622132

Start: 3819802

End: 3820545

Strand: Direct

Name: cmoB [H]

Synonym: Acid_3020

Alternate gene names: 116622132

Gene position: 3819802-3820545 (Clockwise)

Preceding gene: 116622131

Following gene: 116622133

Centisome position: 38.33

GC content: 59.95

Gene sequence:

>744_bases
ATGAAGAGCTCACCTAGTGCCCCCGCGGCACTGCCGAGAGAAGAGATCCGGCGGAGGGTCTCGGAACTCGGGGAGTGGTT
TCACAATCTCGATCTGGACGGGGTGGAGACGGCGCCGAATCACTTTCTAGGCGACTACCCGGCCTGCAAGTGGCGGAACT
TCGCTCACGCGATACCTCGCGATCTGAGCGGTAAAACTGTACTCGATATTGGGTGCAACGCCGGGTTCTACTCGCTCGAA
ATGAAACGGCGAGGCGCGGAGCGGGTGGTTGCCGTCGATTCCGATCCGGTGTATCTGGCCCAGGCGCGATTCGCCGCCGA
GGTCCGCGGCGCAGACATCGAGTTCCGCGAACTGGATGTTTACCGCGTCGATAGCCTGCGGGAGAAATTCGACCTGGTGC
TTTTCATGGGCGTGCTTTACCATTTGCGCCATCCTCTCCTGGCGCTCGATTTATTGTGGGAACACGTGGTGGGCGACACA
CTGGTATTCCAGTCGCTGTTGCGCGGCAGCCGCGAAACGCCGTCGCTGGCCACGGATTACCCATTCTCGGAAACGGGAAT
TTTCGAGCGCGAGGGTTACCCGGCAATGTACTTCGTGGAGCAGAGGTATTCGCACGACCCCACGAACTGGTGGATTCCAA
ATCGCGCGTGTGCCGAAGCTATGCTGCGCAGCGCTGGTTTCGAGATCGCCGCACATCCCGAGGACGAAGTGTACATTTGC
AGGAGAGGCGCCCGCAATGGTTGA

Upstream 100 bases:

>100_bases
GTACGTCGTATCTGGACGTCGGAGCGATGGAGGGCTATGTGGCCGCGATGCAGTCACTGGAAAAACTTGAAATGGACTCC
GCGATGGCAGGAGGCATCCG

Downstream 100 bases:

>100_bases
AGCCGTGATGCTCTGGAACGAGCCGAACAATCTTTCACACTGGAATTTCGAAATCGATCGTGACTGGAGTATTTACGCAA
ACATGGTCAATTTAGCCGCC

Product: type 12 methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MKSSPSAPAALPREEIRRRVSELGEWFHNLDLDGVETAPNHFLGDYPACKWRNFAHAIPRDLSGKTVLDIGCNAGFYSLE
MKRRGAERVVAVDSDPVYLAQARFAAEVRGADIEFRELDVYRVDSLREKFDLVLFMGVLYHLRHPLLALDLLWEHVVGDT
LVFQSLLRGSRETPSLATDYPFSETGIFEREGYPAMYFVEQRYSHDPTNWWIPNRACAEAMLRSAGFEIAAHPEDEVYIC
RRGARNG

Sequences:

>Translated_247_residues
MKSSPSAPAALPREEIRRRVSELGEWFHNLDLDGVETAPNHFLGDYPACKWRNFAHAIPRDLSGKTVLDIGCNAGFYSLE
MKRRGAERVVAVDSDPVYLAQARFAAEVRGADIEFRELDVYRVDSLREKFDLVLFMGVLYHLRHPLLALDLLWEHVVGDT
LVFQSLLRGSRETPSLATDYPFSETGIFEREGYPAMYFVEQRYSHDPTNWWIPNRACAEAMLRSAGFEIAAHPEDEVYIC
RRGARNG
>Mature_247_residues
MKSSPSAPAALPREEIRRRVSELGEWFHNLDLDGVETAPNHFLGDYPACKWRNFAHAIPRDLSGKTVLDIGCNAGFYSLE
MKRRGAERVVAVDSDPVYLAQARFAAEVRGADIEFRELDVYRVDSLREKFDLVLFMGVLYHLRHPLLALDLLWEHVVGDT
LVFQSLLRGSRETPSLATDYPFSETGIFEREGYPAMYFVEQRYSHDPTNWWIPNRACAEAMLRSAGFEIAAHPEDEVYIC
RRGARNG

Specific function: Catalyzes the conversion of 5-hydroxyuridine (ho5U) to 5-methoxyuridine (mo5U) at position 34 in tRNA [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. CmoB family [H]

Homologues:

Organism=Escherichia coli, GI1788178, Length=120, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010017 [H]

Pfam domain/function: PF08003 Methyltransf_9 [H]

EC number: NA

Molecular weight: Translated: 28181; Mature: 28181

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSPSAPAALPREEIRRRVSELGEWFHNLDLDGVETAPNHFLGDYPACKWRNFAHAIPR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHCCC
DLSGKTVLDIGCNAGFYSLEMKRRGAERVVAVDSDPVYLAQARFAAEVRGADIEFRELDV
CCCCCEEEEECCCCCEEEHHHHHCCCCEEEEECCCCEEEHHHHHHHHHCCCCCEEHHCCH
YRVDSLREKFDLVLFMGVLYHLRHPLLALDLLWEHVVGDTLVFQSLLRGSRETPSLATDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PFSETGIFEREGYPAMYFVEQRYSHDPTNWWIPNRACAEAMLRSAGFEIAAHPEDEVYIC
CCCCCCCEECCCCCEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCEEEE
RRGARNG
CCCCCCC
>Mature Secondary Structure
MKSSPSAPAALPREEIRRRVSELGEWFHNLDLDGVETAPNHFLGDYPACKWRNFAHAIPR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHCCC
DLSGKTVLDIGCNAGFYSLEMKRRGAERVVAVDSDPVYLAQARFAAEVRGADIEFRELDV
CCCCCEEEEECCCCCEEEHHHHHCCCCEEEEECCCCEEEHHHHHHHHHCCCCCEEHHCCH
YRVDSLREKFDLVLFMGVLYHLRHPLLALDLLWEHVVGDTLVFQSLLRGSRETPSLATDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PFSETGIFEREGYPAMYFVEQRYSHDPTNWWIPNRACAEAMLRSAGFEIAAHPEDEVYIC
CCCCCCCEECCCCCEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCEEEE
RRGARNG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA