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The map label for this gene is crp [H]

Identifier: 116622046

GI number: 116622046

Start: 3714253

End: 3714963

Strand: Direct

Name: crp [H]

Synonym: Acid_2932

Alternate gene names: 116622046

Gene position: 3714253-3714963 (Clockwise)

Preceding gene: 116622045

Following gene: 116622047

Centisome position: 37.27

GC content: 56.26

Gene sequence:

>711_bases
TTGCTCGAGAATCTGCCGTATTACGAGGAATGGGTGTTAACCAAACTTGCAGCCCTCTCAGAAATCGCGCTGTTCGCCGC
CCTGAGTGAGAACGAACTTCAGGGTCTCGCGCAACGCGCCGTGGAAAAACGATTTGGAACCGATGAGATGCTGTTCTGGG
AAGGCGAGGCATGTGTCGGCATATTCTTGATCATCCAGGGAAGTGTGAAGATCTTTCGGACTTCGCCGGGCGGCCGTGAG
GTTATGCTTTGCATCGAGACCGCGCCGGCATCGGTAGCAGAATTGCCACTCTTTGATGGTGGACCTTATCCGGCATCAGT
TCGAGTGACGGAGCCGGTGGTATCCCTCTTTATTAACAAGAGCGACTTTCAACAGTTCTGCCGCCAGAATCCGGACGTGG
CGCTGAAAGTCCTGGCAGTGGTCGGGCGACGGCTTCGACAGTTGGTGGGACTGGTTGAAGCCATGACCTTTGGGAGCGTT
ACCCAGCGCCTGGCAAAGATGCTTTTGGACGCATCAAAGGAAGCTGGCCCGGAGGACTTCGATTTGCCGGCCACCCACCA
GGAACTCGCATCTCGCCTTGGAACGGTCCGCGAGGTGATCTCCCGGAATCTGGGCCGCTTTCGCGCTCAAGGACTGATCA
AGATTCAGGACCGCCAGGTAGAGATCGTAAATCGCGCGGGACTTCAGCAAGAAGCGGAGTCCCAAGCATAA

Upstream 100 bases:

>100_bases
CCTCCTGTGGCGGCGATGGTCCCGCTCGTTCAGCTACAAGCGATCCCAGCAATGACGCCTCAACTGTAGGGCGCCGGCGA
ATCGTGGTCTCAGCATCAGA

Downstream 100 bases:

>100_bases
TACGCTTTCGGTCGGATCTTTCTCCCTGTGACCACGGTCACCGAAGCTCCATCGCGCGAGTGTGATCATGGAAACAGATG
AAGATGAGACTACAAGGCGA

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MLENLPYYEEWVLTKLAALSEIALFAALSENELQGLAQRAVEKRFGTDEMLFWEGEACVGIFLIIQGSVKIFRTSPGGRE
VMLCIETAPASVAELPLFDGGPYPASVRVTEPVVSLFINKSDFQQFCRQNPDVALKVLAVVGRRLRQLVGLVEAMTFGSV
TQRLAKMLLDASKEAGPEDFDLPATHQELASRLGTVREVISRNLGRFRAQGLIKIQDRQVEIVNRAGLQQEAESQA

Sequences:

>Translated_236_residues
MLENLPYYEEWVLTKLAALSEIALFAALSENELQGLAQRAVEKRFGTDEMLFWEGEACVGIFLIIQGSVKIFRTSPGGRE
VMLCIETAPASVAELPLFDGGPYPASVRVTEPVVSLFINKSDFQQFCRQNPDVALKVLAVVGRRLRQLVGLVEAMTFGSV
TQRLAKMLLDASKEAGPEDFDLPATHQELASRLGTVREVISRNLGRFRAQGLIKIQDRQVEIVNRAGLQQEAESQA
>Mature_236_residues
MLENLPYYEEWVLTKLAALSEIALFAALSENELQGLAQRAVEKRFGTDEMLFWEGEACVGIFLIIQGSVKIFRTSPGGRE
VMLCIETAPASVAELPLFDGGPYPASVRVTEPVVSLFINKSDFQQFCRQNPDVALKVLAVVGRRLRQLVGLVEAMTFGSV
TQRLAKMLLDASKEAGPEDFDLPATHQELASRLGTVREVISRNLGRFRAQGLIKIQDRQVEIVNRAGLQQEAESQA

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA. Acts as a negative regulator of its own synthesis

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=182, Percent_Identity=26.9230769230769, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26094; Mature: 26094

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENLPYYEEWVLTKLAALSEIALFAALSENELQGLAQRAVEKRFGTDEMLFWEGEACVG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEECCCHHEE
IFLIIQGSVKIFRTSPGGREVMLCIETAPASVAELPLFDGGPYPASVRVTEPVVSLFINK
EEEEEECCEEEEEECCCCCEEEEEEECCCCCHHHCCCCCCCCCCCCEEHHHHHHHHHHCH
SDFQQFCRQNPDVALKVLAVVGRRLRQLVGLVEAMTFGSVTQRLAKMLLDASKEAGPEDF
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DLPATHQELASRLGTVREVISRNLGRFRAQGLIKIQDRQVEIVNRAGLQQEAESQA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MLENLPYYEEWVLTKLAALSEIALFAALSENELQGLAQRAVEKRFGTDEMLFWEGEACVG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEECCCHHEE
IFLIIQGSVKIFRTSPGGREVMLCIETAPASVAELPLFDGGPYPASVRVTEPVVSLFINK
EEEEEECCEEEEEECCCCCEEEEEEECCCCCHHHCCCCCCCCCCCCEEHHHHHHHHHHCH
SDFQQFCRQNPDVALKVLAVVGRRLRQLVGLVEAMTFGSVTQRLAKMLLDASKEAGPEDF
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DLPATHQELASRLGTVREVISRNLGRFRAQGLIKIQDRQVEIVNRAGLQQEAESQA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]