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The map label for this gene is glgX [H]

Identifier: 116622006

GI number: 116622006

Start: 3664470

End: 3666560

Strand: Reverse

Name: glgX [H]

Synonym: Acid_2891

Alternate gene names: 116622006

Gene position: 3666560-3664470 (Counterclockwise)

Preceding gene: 116622007

Following gene: 116622002

Centisome position: 36.79

GC content: 63.18

Gene sequence:

>2091_bases
ATGACCTCTAACCTCCTCGAAAACCCGCAATCCGCACCTCCCTGCACAGCCGCACTCGCCGGACGCTGCTCCCCTCTCGG
TGCCACCGTGCAGGCCAATGGTGTCAACTTCAGCGTCTTCTCGCGCTCCGCCTCCGCCATCGAGTTGCTCCTCTTCGATC
GCGAAGACGATGCCCTCCCCGCGCGCGTCATCCGCATCCCCGCCGCCACCAATCGCACCTACCACTACTGGCATACCTTC
GTCCCCGGCGCCCGCCCCGGCCAGCTCTACGGCTACCGCGTCCACGGGCCCTTCGACCCCGCCTCCGGATTGCGCTTCGA
TCCCCGTAAACTCCTCCTCGATCCGTACGGACGCGGCATCGTTGTCCCCGCCGGGTACAGCCGCGAAGCCGCCCGCCTTC
CCGGCGATAGCGCGCGCACCGCCATGAAAAGCGTCGTCATCGATCCCGCCGCCTACGATTGGGAGGGTGACACGCCCCTT
CACCGCCCCTCCTCTCGCACCATCATCTATGAACTCCACGTCCGCGGCTTCACCCGCCATCCCAGCTCCGGCCTTTCCGA
AAACCTTCGCGGCACCTACGCCGGCCTGATCGAAAAAATCCCCTACCTGCACGATCTAGGCATCACCGCCGTGGAGCTCA
TGCCGGTCTTCCAGTTCGATCCCCAGGATGCCCCGGTCGGACACTGCAACTATTGGGGCTACGCACCCGTCTCCTTCTTC
GCGCCCCATCAGCAATACAGTTCGCGCCGCGACCCCCAGGGCGTCCTCAACGAGTTCCGCGATATGGTGAAGGCCCTGCA
CCGCGCCGGCATCGAAGTCATCCTCGACGTCGTTTTCAATCACACCGCCGAGGGCGACCATCGCGGGCCTACCCTCAGTT
TCCGCGGCTTCGATAACACCGCCTACTACCTCCTCGAGCCCGATCGCTCGTACTCCAATTACAGCGGCACCGGCAACACC
CTCAACGCCAATCACCCCATCGTTCGCCGCATGATCGTCGATAGCCTCCGCTACTGGGTGGAGGAAATGCACATCGATGG
CTTCCGTTTCGATCTCGCCTCCATCCTCTCGCGCGATTGCTGCGGCCGCCCTATGGCCCAGCCGCCCGTCCTCTGGGACA
TCGAATGCGACGCCGTCCTCGCCGGCACCAAGCTCATCGCCGAAGCCTGGGACGCCGCCGGCCTCTATCAGGTCGGCAGC
TTCATCGGCGATAGCTGGCGCGAATGGAATGGCCGCTTTCGCGACGATATCCGTGCCTTCGTCCGCGGTGACGACGATAC
CGCCGCCCGCTTCGCCGACCGCTGGTTAGGCAGCCAGGAAATTTACGGCCATGAGAATCGCGAGGCCGAACAGAGCATCA
ACTTCGTCACCTGCCACGATGGCTTCACGCTTAACGACCTGGTCTCCTATAACGACAAACACAACCAGCAGAACGGCGAA
AGCAATCGCGATGGCTGCAACGACAATCGTAGTTGGAACTGCGGCGTCGAAGGACCTTCCAACGACCCCGAAATCGAGGC
CCTGCGCAACCGCCAGATCAAGAACTTCCTCACCATCACCCTGCTCTCCCTCGGCGTCCCCATGATCGTGATGGGAGATG
AAGTCCGGCGCACCCAGCAAGGAAACAACAACGCGTATAGCCAGGATAATGAAACCAGTTGGTTCGATTGGACACTCGTC
CAGCGCCACGCCGATCTACATCGCTTCGTCAAACTGCTGATCGCCCGCCGCCTCCTGCGCGATATCGATAGCGACCGCAC
TACTCTGAATGAACTGCTCCACGGCGCCCGAAAGGCCTGGCACGGCGTAACCCTCTTCGAGCCCGATTGGAGTCCCTGGT
CGCACAGCCTCGCCCTGGGCGCCGAGCTGTTCAAAGAGCGCCTGGAGTTCCACATGATCTTCAACTCCTGGTGGCAGCCC
CTCGATTTCGAGCTTCCCCCCGTGCTCTCGCGCTCTCCCTGGCGCCGCTGGATTGATACCGCCCAGCCCTCGCCGCGCGA
CATCGTCGATTGGCAAGCCGCTTCCCCAATCGACGGCCTCCACTACCACGCTGCCCCCCGCTCCGTCGTCGTCCTCATCC
GCGACCGGTAA

Upstream 100 bases:

>100_bases
CTGCCCCCTCCGTTCGAACTCCAACTCCAGGAGATCGCCTGATGCGTCAACGAGCCGCGAACCGCGACCGTTAGGGAGCG
ATCTCTTCTGGAGCTGACTC

Downstream 100 bases:

>100_bases
GGCAGGCCTCCTGGCCTGTCTCCGCGGGCCGCGAAGATTCTTGTGGGGCAGCCACTCTTGCGCAGCCGCCTTTTAGGCGG
CTTTCCCGATACACCACGCA

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 696; Mature: 695

Protein sequence:

>696_residues
MTSNLLENPQSAPPCTAALAGRCSPLGATVQANGVNFSVFSRSASAIELLLFDREDDALPARVIRIPAATNRTYHYWHTF
VPGARPGQLYGYRVHGPFDPASGLRFDPRKLLLDPYGRGIVVPAGYSREAARLPGDSARTAMKSVVIDPAAYDWEGDTPL
HRPSSRTIIYELHVRGFTRHPSSGLSENLRGTYAGLIEKIPYLHDLGITAVELMPVFQFDPQDAPVGHCNYWGYAPVSFF
APHQQYSSRRDPQGVLNEFRDMVKALHRAGIEVILDVVFNHTAEGDHRGPTLSFRGFDNTAYYLLEPDRSYSNYSGTGNT
LNANHPIVRRMIVDSLRYWVEEMHIDGFRFDLASILSRDCCGRPMAQPPVLWDIECDAVLAGTKLIAEAWDAAGLYQVGS
FIGDSWREWNGRFRDDIRAFVRGDDDTAARFADRWLGSQEIYGHENREAEQSINFVTCHDGFTLNDLVSYNDKHNQQNGE
SNRDGCNDNRSWNCGVEGPSNDPEIEALRNRQIKNFLTITLLSLGVPMIVMGDEVRRTQQGNNNAYSQDNETSWFDWTLV
QRHADLHRFVKLLIARRLLRDIDSDRTTLNELLHGARKAWHGVTLFEPDWSPWSHSLALGAELFKERLEFHMIFNSWWQP
LDFELPPVLSRSPWRRWIDTAQPSPRDIVDWQAASPIDGLHYHAAPRSVVVLIRDR

Sequences:

>Translated_696_residues
MTSNLLENPQSAPPCTAALAGRCSPLGATVQANGVNFSVFSRSASAIELLLFDREDDALPARVIRIPAATNRTYHYWHTF
VPGARPGQLYGYRVHGPFDPASGLRFDPRKLLLDPYGRGIVVPAGYSREAARLPGDSARTAMKSVVIDPAAYDWEGDTPL
HRPSSRTIIYELHVRGFTRHPSSGLSENLRGTYAGLIEKIPYLHDLGITAVELMPVFQFDPQDAPVGHCNYWGYAPVSFF
APHQQYSSRRDPQGVLNEFRDMVKALHRAGIEVILDVVFNHTAEGDHRGPTLSFRGFDNTAYYLLEPDRSYSNYSGTGNT
LNANHPIVRRMIVDSLRYWVEEMHIDGFRFDLASILSRDCCGRPMAQPPVLWDIECDAVLAGTKLIAEAWDAAGLYQVGS
FIGDSWREWNGRFRDDIRAFVRGDDDTAARFADRWLGSQEIYGHENREAEQSINFVTCHDGFTLNDLVSYNDKHNQQNGE
SNRDGCNDNRSWNCGVEGPSNDPEIEALRNRQIKNFLTITLLSLGVPMIVMGDEVRRTQQGNNNAYSQDNETSWFDWTLV
QRHADLHRFVKLLIARRLLRDIDSDRTTLNELLHGARKAWHGVTLFEPDWSPWSHSLALGAELFKERLEFHMIFNSWWQP
LDFELPPVLSRSPWRRWIDTAQPSPRDIVDWQAASPIDGLHYHAAPRSVVVLIRDR
>Mature_695_residues
TSNLLENPQSAPPCTAALAGRCSPLGATVQANGVNFSVFSRSASAIELLLFDREDDALPARVIRIPAATNRTYHYWHTFV
PGARPGQLYGYRVHGPFDPASGLRFDPRKLLLDPYGRGIVVPAGYSREAARLPGDSARTAMKSVVIDPAAYDWEGDTPLH
RPSSRTIIYELHVRGFTRHPSSGLSENLRGTYAGLIEKIPYLHDLGITAVELMPVFQFDPQDAPVGHCNYWGYAPVSFFA
PHQQYSSRRDPQGVLNEFRDMVKALHRAGIEVILDVVFNHTAEGDHRGPTLSFRGFDNTAYYLLEPDRSYSNYSGTGNTL
NANHPIVRRMIVDSLRYWVEEMHIDGFRFDLASILSRDCCGRPMAQPPVLWDIECDAVLAGTKLIAEAWDAAGLYQVGSF
IGDSWREWNGRFRDDIRAFVRGDDDTAARFADRWLGSQEIYGHENREAEQSINFVTCHDGFTLNDLVSYNDKHNQQNGES
NRDGCNDNRSWNCGVEGPSNDPEIEALRNRQIKNFLTITLLSLGVPMIVMGDEVRRTQQGNNNAYSQDNETSWFDWTLVQ
RHADLHRFVKLLIARRLLRDIDSDRTTLNELLHGARKAWHGVTLFEPDWSPWSHSLALGAELFKERLEFHMIFNSWWQPL
DFELPPVLSRSPWRRWIDTAQPSPRDIVDWQAASPIDGLHYHAAPRSVVVLIRDR

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=580, Percent_Identity=45.5172413793103, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1789839, Length=335, Percent_Identity=27.7611940298507, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 78898; Mature: 78767

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSNLLENPQSAPPCTAALAGRCSPLGATVQANGVNFSVFSRSASAIELLLFDREDDALP
CCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEECCEEEEEECCCCCEEEEEEECCCCCCCC
ARVIRIPAATNRTYHYWHTFVPGARPGQLYGYRVHGPFDPASGLRFDPRKLLLDPYGRGI
EEEEEECCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCCEE
VVPAGYSREAARLPGDSARTAMKSVVIDPAAYDWEGDTPLHRPSSRTIIYELHVRGFTRH
EEECCCCCCHHCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCC
PSSGLSENLRGTYAGLIEKIPYLHDLGITAVELMPVFQFDPQDAPVGHCNYWGYAPVSFF
CCCCHHHHHHHHHHHHHHHCCCHHHCCCEEEEEEEHEEECCCCCCCCCCCCCCCCCHHHC
APHQQYSSRRDPQGVLNEFRDMVKALHRAGIEVILDVVFNHTAEGDHRGPTLSFRGFDNT
CCHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCE
AYYLLEPDRSYSNYSGTGNTLNANHPIVRRMIVDSLRYWVEEMHIDGFRFDLASILSRDC
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
CGRPMAQPPVLWDIECDAVLAGTKLIAEAWDAAGLYQVGSFIGDSWREWNGRFRDDIRAF
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCHHHHHHHH
VRGDDDTAARFADRWLGSQEIYGHENREAEQSINFVTCHDGFTLNDLVSYNDKHNQQNGE
HCCCCHHHHHHHHHHCCCCHHCCCCCCCHHHCCCEEEECCCCCHHHHHHCCCCCCCCCCC
SNRDGCNDNRSWNCGVEGPSNDPEIEALRNRQIKNFLTITLLSLGVPMIVMGDEVRRTQQ
CCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHC
GNNNAYSQDNETSWFDWTLVQRHADLHRFVKLLIARRLLRDIDSDRTTLNELLHGARKAW
CCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
HGVTLFEPDWSPWSHSLALGAELFKERLEFHMIFNSWWQPLDFELPPVLSRSPWRRWIDT
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHC
AQPSPRDIVDWQAASPIDGLHYHAAPRSVVVLIRDR
CCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEEECC
>Mature Secondary Structure 
TSNLLENPQSAPPCTAALAGRCSPLGATVQANGVNFSVFSRSASAIELLLFDREDDALP
CCCCCCCCCCCCCHHHHHHCCCCCCCCEEEECCEEEEEECCCCCEEEEEEECCCCCCCC
ARVIRIPAATNRTYHYWHTFVPGARPGQLYGYRVHGPFDPASGLRFDPRKLLLDPYGRGI
EEEEEECCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCCEE
VVPAGYSREAARLPGDSARTAMKSVVIDPAAYDWEGDTPLHRPSSRTIIYELHVRGFTRH
EEECCCCCCHHCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCC
PSSGLSENLRGTYAGLIEKIPYLHDLGITAVELMPVFQFDPQDAPVGHCNYWGYAPVSFF
CCCCHHHHHHHHHHHHHHHCCCHHHCCCEEEEEEEHEEECCCCCCCCCCCCCCCCCHHHC
APHQQYSSRRDPQGVLNEFRDMVKALHRAGIEVILDVVFNHTAEGDHRGPTLSFRGFDNT
CCHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCE
AYYLLEPDRSYSNYSGTGNTLNANHPIVRRMIVDSLRYWVEEMHIDGFRFDLASILSRDC
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
CGRPMAQPPVLWDIECDAVLAGTKLIAEAWDAAGLYQVGSFIGDSWREWNGRFRDDIRAF
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCHHHHHHHH
VRGDDDTAARFADRWLGSQEIYGHENREAEQSINFVTCHDGFTLNDLVSYNDKHNQQNGE
HCCCCHHHHHHHHHHCCCCHHCCCCCCCHHHCCCEEEECCCCCHHHHHHCCCCCCCCCCC
SNRDGCNDNRSWNCGVEGPSNDPEIEALRNRQIKNFLTITLLSLGVPMIVMGDEVRRTQQ
CCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHC
GNNNAYSQDNETSWFDWTLVQRHADLHRFVKLLIARRLLRDIDSDRTTLNELLHGARKAW
CCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
HGVTLFEPDWSPWSHSLALGAELFKERLEFHMIFNSWWQPLDFELPPVLSRSPWRRWIDT
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHC
AQPSPRDIVDWQAASPIDGLHYHAAPRSVVVLIRDR
CCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]