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The map label for this gene is sigW [H]

Identifier: 116621996

GI number: 116621996

Start: 3649938

End: 3650687

Strand: Reverse

Name: sigW [H]

Synonym: Acid_2881

Alternate gene names: 116621996

Gene position: 3650687-3649938 (Counterclockwise)

Preceding gene: 116621997

Following gene: 116621995

Centisome position: 36.63

GC content: 58.53

Gene sequence:

>750_bases
ATGTTCGCGATAGCAAAAGATTGCTCCTCCCCCCAATCGGAAACTACGCCAACCGCCACGCCTGCAAGCGAGGCTCCCGG
CGCCGCCGTCAAGAACTCTCATGACGAACCGGCCACCGAGGGCGTCACCAGCGACGAGACCTTGGTTGATCGTGCCAGAA
AGGGGGATAAACTGGCCTTCGGCGAGTTGATCCAGCGGCATCGTAATGCCTGCCTCAAACGGGCACGCCTCATGATCCGC
GATCGCAGCGACGCCGAGGACGAAGTTCAAAACGCCTTCTGGAAGGCATTTCTGCGCCTCGATCAATATCGGGGCGAAGG
GAGTTTCGGTGCATGGCTTAGCCGTATTGTGGAAAACCAATGCCTGATGCGTATTCGCGATGAGCGCAACGTTCGCTTCG
TCTACCTGGACGAACCCACCGAATCCAATGTCCGGTTGGAAGTAGTGGGGCAGAGCCCCGACCCGGAAGGACAACTGGGT
TTGAGCGAAGTCGGGATACTGCTAAAGAGGGAAATCAACCGCATTCCACCCCTGTTGCGGAATGTGATGCTGCTCCGCGA
TGTCGATCAATTGCCGATGTTCGAAATCGCCGGCAGACTGGGTTTGTCGTTGCCCGCCGCCAAATCCAGATTGCGGCGCG
CCCGCATGGAAATGAAGTCGCGCATCTCGTCCCATTGCGGACGCAGGGGCGTCGGCACGCTTACCCAAACCGCCAAGTAC
AATCAGGCGGCTTACACTCGCGCCAGTTAG

Upstream 100 bases:

>100_bases
TTCCCTAATCCACTTCCGCATAGTACTTTTGTATCGTTTTGATTCTAAAGTCGCCACATTTGTACTAGGCCTTGGCCCCC
GGAGTGCGATTACGCTGTGC

Downstream 100 bases:

>100_bases
CATGGGCAACCTCCAAATGCCGCGGGGTCCTGCTCCTTTGCGGGGCCTCGCGGCGGCGCGTGGTCTCAACATATAATCCA
TCTCGCTGAGCAAAAACAGC

Product: ECF subfamily RNA polymerase sigma-24 factor

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MFAIAKDCSSPQSETTPTATPASEAPGAAVKNSHDEPATEGVTSDETLVDRARKGDKLAFGELIQRHRNACLKRARLMIR
DRSDAEDEVQNAFWKAFLRLDQYRGEGSFGAWLSRIVENQCLMRIRDERNVRFVYLDEPTESNVRLEVVGQSPDPEGQLG
LSEVGILLKREINRIPPLLRNVMLLRDVDQLPMFEIAGRLGLSLPAAKSRLRRARMEMKSRISSHCGRRGVGTLTQTAKY
NQAAYTRAS

Sequences:

>Translated_249_residues
MFAIAKDCSSPQSETTPTATPASEAPGAAVKNSHDEPATEGVTSDETLVDRARKGDKLAFGELIQRHRNACLKRARLMIR
DRSDAEDEVQNAFWKAFLRLDQYRGEGSFGAWLSRIVENQCLMRIRDERNVRFVYLDEPTESNVRLEVVGQSPDPEGQLG
LSEVGILLKREINRIPPLLRNVMLLRDVDQLPMFEIAGRLGLSLPAAKSRLRRARMEMKSRISSHCGRRGVGTLTQTAKY
NQAAYTRAS
>Mature_249_residues
MFAIAKDCSSPQSETTPTATPASEAPGAAVKNSHDEPATEGVTSDETLVDRARKGDKLAFGELIQRHRNACLKRARLMIR
DRSDAEDEVQNAFWKAFLRLDQYRGEGSFGAWLSRIVENQCLMRIRDERNVRFVYLDEPTESNVRLEVVGQSPDPEGQLG
LSEVGILLKREINRIPPLLRNVMLLRDVDQLPMFEIAGRLGLSLPAAKSRLRRARMEMKSRISSHCGRRGVGTLTQTAKY
NQAAYTRAS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=190, Percent_Identity=31.0526315789474, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 27913; Mature: 27913

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAIAKDCSSPQSETTPTATPASEAPGAAVKNSHDEPATEGVTSDETLVDRARKGDKLAF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHCCCCHHH
GELIQRHRNACLKRARLMIRDRSDAEDEVQNAFWKAFLRLDQYRGEGSFGAWLSRIVENQ
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
CLMRIRDERNVRFVYLDEPTESNVRLEVVGQSPDPEGQLGLSEVGILLKREINRIPPLLR
HHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHH
NVMLLRDVDQLPMFEIAGRLGLSLPAAKSRLRRARMEMKSRISSHCGRRGVGTLTQTAKY
HHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
NQAAYTRAS
CHHHHCCCC
>Mature Secondary Structure
MFAIAKDCSSPQSETTPTATPASEAPGAAVKNSHDEPATEGVTSDETLVDRARKGDKLAF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHCCCCHHH
GELIQRHRNACLKRARLMIRDRSDAEDEVQNAFWKAFLRLDQYRGEGSFGAWLSRIVENQ
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
CLMRIRDERNVRFVYLDEPTESNVRLEVVGQSPDPEGQLGLSEVGILLKREINRIPPLLR
HHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHH
NVMLLRDVDQLPMFEIAGRLGLSLPAAKSRLRRARMEMKSRISSHCGRRGVGTLTQTAKY
HHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
NQAAYTRAS
CHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]