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The map label for this gene is agaA [H]

Identifier: 116621935

GI number: 116621935

Start: 3574061

End: 3575548

Strand: Reverse

Name: agaA [H]

Synonym: Acid_2820

Alternate gene names: 116621935

Gene position: 3575548-3574061 (Counterclockwise)

Preceding gene: 116621940

Following gene: 116621934

Centisome position: 35.88

GC content: 62.9

Gene sequence:

>1488_bases
ATGAAACTATCCTTCGTCTTTTTCTGTGCGGCCTCCTGTTTCGCGGCCGACCTAAGCGGCAATTGGGCCTCTGGCAATGC
CGGAGTCGATGGCATTGCCCGCAAGGTCTACTTCAACTTGAAGCAGGATGGCTCGCGCATCACGGGCCACATCCGCTCCA
CGCAGTTTTACTACACCATCAAGGAAAGCACCGGCGACGCATCGGGCTTCACGCTCACGGCCAGCATGATGGATGGCAAG
AGTGAACGCACGGTCCGCTACGAAGGCAAGCTCGAAGGCGAAGAACTCCACCTGGGCACTCGCCGCAATGCCACCGCGCC
GCTCAGCCAGCTGGTGGCGCGCCGCGTGCCCGAGGGCGAAGGCGCGTATCCTGCCCGCATTCCTCCGCCCGCCCTGCATA
AGGTGCCCGCCAACGGCCTGGCCAAGACGCCGCCGATGGGTTGGAACAGCTGGAACAAATTCGCCGGCCGCGTAGACGAT
AAGAGCGTCCGCGACATGGCCGACGCGATCGTGACCAGCGGCATGCGCGACGCGGGCTACGTCTACGTGAATATCGACGA
TACCTGGGAGGGCGAGCGGGACGCCAAGGGTAATATCACATCGAACAAGAAGTTCCCCGACATGAAAGCGCTGGCCGATT
ACGTCCACGGCAAGGGCCTCAAGCTCGGCATTTACTCTTCGCCCGGGCCCAACACCTGCGCCGGTTACGAGGGCAGCTAC
GGACACGAAGAGCAGGACGCGAAGACCTGGGCGGCCTGGGGGATCGATTACCTGAAATACGATTGGTGCGGCGCGCGCAA
CCTTTACACCGACGACGAAATGCAGGCCGTGTACCAGAAGATGGGCGATGCCCTGCGCGCCTCCGGCCGTCCGATCCTGT
ACAGCCTCTGCCAGTACGGCCGCGCGGACGTTTGGAAGTGGGGACCCGAAGTGGGAGGTAACGCGTGGCGGACCACCGGC
GATATCAAGGACACGTGGGATTCCATGACTAATATCGGGTTCAAGCAAAACGAACTCGCCCAATGGGCCGCGCCCGGCCA
CTGGAACGACCCGGACATGCTCGAGATCGGCAATGGCGGCATGAACGACGACGAATATCGCACGCACATGAGCCTCTGGT
CGATGCTCGCGGCGCCCCTGCTGGCGGGCAACGATCTGCGCACGGCTTCGCCCGCAATCCTCGCGATTCTGACCAATCGC
GACGTGATCGCGATCGACCAGGACAAGGATGGCAAGCAGGGCCGTCGCGCGTGGAAATCCGGCGATCAGGAGATCTGGGT
CCGCGACCTCGCCGGTGGCGATAGGGCGGTAGCGATCTTCAATCGCGGCGCGGAGAAGGCCGACGTGAAGATGAAGTGGA
GCGAGCTGGAAGTGAACGCGCCGGCGCAGGCGAAAAACCTCTGGTCGCACCAGGAGGAGAAACTCCAGGGCAGCGAGTTT
GCCCTGTCAGTGCCCGCTCACGGTGTCGTGCTGCTGCGCGTGCGGTAA

Upstream 100 bases:

>100_bases
TCCAAAATGCGGGTCTCATGCCGGAGTGACGCACTCTGTGAGCAAAGCGTGGAGAACGGTCGTCTTTAGCGCTATATAGG
CTATAGTAGGGAGGAATCCG

Downstream 100 bases:

>100_bases
GGGTCAGGAGATCCTCCCTCACGGTCGCGGCTCGTTACAATGAGGCCTAAATGAAAGCGGTCGATAGAGCGGTCCCACCG
GATGTACGCCAGCGGTACGC

Product: alpha-galactosidase

Products: NA

Alternate protein names: Alpha-D-galactoside galactohydrolase; Alpha-galactosidase 27A; Melibiase [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MKLSFVFFCAASCFAADLSGNWASGNAGVDGIARKVYFNLKQDGSRITGHIRSTQFYYTIKESTGDASGFTLTASMMDGK
SERTVRYEGKLEGEELHLGTRRNATAPLSQLVARRVPEGEGAYPARIPPPALHKVPANGLAKTPPMGWNSWNKFAGRVDD
KSVRDMADAIVTSGMRDAGYVYVNIDDTWEGERDAKGNITSNKKFPDMKALADYVHGKGLKLGIYSSPGPNTCAGYEGSY
GHEEQDAKTWAAWGIDYLKYDWCGARNLYTDDEMQAVYQKMGDALRASGRPILYSLCQYGRADVWKWGPEVGGNAWRTTG
DIKDTWDSMTNIGFKQNELAQWAAPGHWNDPDMLEIGNGGMNDDEYRTHMSLWSMLAAPLLAGNDLRTASPAILAILTNR
DVIAIDQDKDGKQGRRAWKSGDQEIWVRDLAGGDRAVAIFNRGAEKADVKMKWSELEVNAPAQAKNLWSHQEEKLQGSEF
ALSVPAHGVVLLRVR

Sequences:

>Translated_495_residues
MKLSFVFFCAASCFAADLSGNWASGNAGVDGIARKVYFNLKQDGSRITGHIRSTQFYYTIKESTGDASGFTLTASMMDGK
SERTVRYEGKLEGEELHLGTRRNATAPLSQLVARRVPEGEGAYPARIPPPALHKVPANGLAKTPPMGWNSWNKFAGRVDD
KSVRDMADAIVTSGMRDAGYVYVNIDDTWEGERDAKGNITSNKKFPDMKALADYVHGKGLKLGIYSSPGPNTCAGYEGSY
GHEEQDAKTWAAWGIDYLKYDWCGARNLYTDDEMQAVYQKMGDALRASGRPILYSLCQYGRADVWKWGPEVGGNAWRTTG
DIKDTWDSMTNIGFKQNELAQWAAPGHWNDPDMLEIGNGGMNDDEYRTHMSLWSMLAAPLLAGNDLRTASPAILAILTNR
DVIAIDQDKDGKQGRRAWKSGDQEIWVRDLAGGDRAVAIFNRGAEKADVKMKWSELEVNAPAQAKNLWSHQEEKLQGSEF
ALSVPAHGVVLLRVR
>Mature_495_residues
MKLSFVFFCAASCFAADLSGNWASGNAGVDGIARKVYFNLKQDGSRITGHIRSTQFYYTIKESTGDASGFTLTASMMDGK
SERTVRYEGKLEGEELHLGTRRNATAPLSQLVARRVPEGEGAYPARIPPPALHKVPANGLAKTPPMGWNSWNKFAGRVDD
KSVRDMADAIVTSGMRDAGYVYVNIDDTWEGERDAKGNITSNKKFPDMKALADYVHGKGLKLGIYSSPGPNTCAGYEGSY
GHEEQDAKTWAAWGIDYLKYDWCGARNLYTDDEMQAVYQKMGDALRASGRPILYSLCQYGRADVWKWGPEVGGNAWRTTG
DIKDTWDSMTNIGFKQNELAQWAAPGHWNDPDMLEIGNGGMNDDEYRTHMSLWSMLAAPLLAGNDLRTASPAILAILTNR
DVIAIDQDKDGKQGRRAWKSGDQEIWVRDLAGGDRAVAIFNRGAEKADVKMKWSELEVNAPAQAKNLWSHQEEKLQGSEF
ALSVPAHGVVLLRVR

Specific function: Hydrolyzes galactomannan found in plant cell wall, by cleaving alpha-1,6-D-galactose side-chains from the mannan backbone. Appears to act in synergy with mannanase (ManA) to elicit hydrolysis of galactomannan. Has greater activity against galactomannans w

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 27 family [H]

Homologues:

Organism=Homo sapiens, GI4504009, Length=330, Percent_Identity=41.5151515151515, Blast_Score=239, Evalue=5e-63,
Organism=Homo sapiens, GI4557781, Length=336, Percent_Identity=41.0714285714286, Blast_Score=220, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17562946, Length=415, Percent_Identity=35.421686746988, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24583253, Length=394, Percent_Identity=37.5634517766497, Blast_Score=240, Evalue=2e-63,
Organism=Drosophila melanogaster, GI19922382, Length=350, Percent_Identity=37.1428571428571, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24654142, Length=350, Percent_Identity=37.1428571428571, Blast_Score=220, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013780
- InterPro:   IPR002241
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 54628; Mature: 54628

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00512 ALPHA_GALACTOSIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSFVFFCAASCFAADLSGNWASGNAGVDGIARKVYFNLKQDGSRITGHIRSTQFYYTI
CCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHEEEEEEEECCCCCEEEEEEEEEEEEEEE
KESTGDASGFTLTASMMDGKSERTVRYEGKLEGEELHLGTRRNATAPLSQLVARRVPEGE
ECCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCC
GAYPARIPPPALHKVPANGLAKTPPMGWNSWNKFAGRVDDKSVRDMADAIVTSGMRDAGY
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCE
VYVNIDDTWEGERDAKGNITSNKKFPDMKALADYVHGKGLKLGIYSSPGPNTCAGYEGSY
EEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
GHEEQDAKTWAAWGIDYLKYDWCGARNLYTDDEMQAVYQKMGDALRASGRPILYSLCQYG
CCCHHHHHHHHHHCCHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
RADVWKWGPEVGGNAWRTTGDIKDTWDSMTNIGFKQNELAQWAAPGHWNDPDMLEIGNGG
CCCCEECCCCCCCCCEEECCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEEECCCC
MNDDEYRTHMSLWSMLAAPLLAGNDLRTASPAILAILTNRDVIAIDQDKDGKQGRRAWKS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEECCCCCHHHHHHHHC
GDQEIWVRDLAGGDRAVAIFNRGAEKADVKMKWSELEVNAPAQAKNLWSHQEEKLQGSEF
CCCEEEEEECCCCCEEEEEEECCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCE
ALSVPAHGVVLLRVR
EEECCCCCEEEEEEC
>Mature Secondary Structure
MKLSFVFFCAASCFAADLSGNWASGNAGVDGIARKVYFNLKQDGSRITGHIRSTQFYYTI
CCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHEEEEEEEECCCCCEEEEEEEEEEEEEEE
KESTGDASGFTLTASMMDGKSERTVRYEGKLEGEELHLGTRRNATAPLSQLVARRVPEGE
ECCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCC
GAYPARIPPPALHKVPANGLAKTPPMGWNSWNKFAGRVDDKSVRDMADAIVTSGMRDAGY
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCE
VYVNIDDTWEGERDAKGNITSNKKFPDMKALADYVHGKGLKLGIYSSPGPNTCAGYEGSY
EEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
GHEEQDAKTWAAWGIDYLKYDWCGARNLYTDDEMQAVYQKMGDALRASGRPILYSLCQYG
CCCHHHHHHHHHHCCHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
RADVWKWGPEVGGNAWRTTGDIKDTWDSMTNIGFKQNELAQWAAPGHWNDPDMLEIGNGG
CCCCEECCCCCCCCCEEECCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEEECCCC
MNDDEYRTHMSLWSMLAAPLLAGNDLRTASPAILAILTNRDVIAIDQDKDGKQGRRAWKS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEECCCCCHHHHHHHHC
GDQEIWVRDLAGGDRAVAIFNRGAEKADVKMKWSELEVNAPAQAKNLWSHQEEKLQGSEF
CCCEEEEEECCCCCEEEEEEECCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCE
ALSVPAHGVVLLRVR
EEECCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA