Click here to switch to the map view.

The map label for this gene is carA [H]

Identifier: 116621512

GI number: 116621512

Start: 3049276

End: 3050403

Strand: Direct

Name: carA [H]

Synonym: Acid_2394

Alternate gene names: 116621512

Gene position: 3049276-3050403 (Clockwise)

Preceding gene: 116621509

Following gene: 116621513

Centisome position: 30.6

GC content: 61.52

Gene sequence:

>1128_bases
TTGAACACTGCAATCCTCGCCCTGGAAGACGGCAGCGTCTTCGAGGGCCGGAGTTTTGGTGCGCCCACAGAGCGGAGCGG
GGAAGTTGTCTTCAATACCGCTCTCACCGGATACCAGGAAGTCTTTACCGACCCTTCGTATTCCGGCCAGATTGTCATCC
TGACCAACCCCCAAATCGGGAACTACGGCACCAGTTCGCGCGACAACGAATCCAAGGGGCCGTACATCGAAGGGCTGGTT
GTCCGCGAGTTCTCCTCGATCGCGAGCAACTGGCGATCGGATTCGTCGGCCGATCGCTTTCTCGCCAACTCCGGAGTCCC
GATCGTCAGCGATCTGGATACGCGCGCGCTGGTGCGTCACCTGCGCACCCGCGGTGTGATGCGCGGCGTGCTTTCCGCCG
TGGAAAGCGACGCGCAGAAGCTGGTCGAAAAAGCACGCAACATTCCGACCATGACCGGCCTCGATCTCGCCAGCCGCGTG
ACCACCGCCGAGCGCTACGAGTGGACCAAGACGGTGGAACAGTGCTCGCCCTCCGACCTGGTCGGCAAGACCGCGGAGCC
GCTCTATAACGTGGTGGCCTACGATTTCGGCATCAAGCACAACATTCTGCGCCGCCTGGTCCACGCAGGCTGCCGCGTTA
CCGTGGTCCCCGCGCTCACCAGCGCCGAAGATGTGCTCGCGCTGAACCCCGACGGCATCTTCCTCTCGAACGGGCCGGGC
GACCCCGAACCGCTGGTTCCGCAGGTCAACAACATTCGCAAGCTGATTGGAAAGAAGCCCATTTTCGGCATCTGCCTGGG
TAACCAGCTCCTCGGGCTCGCCATGGGCGGCAAGACGTACAAGCTGAAGTTCGGACATCGCGGCGCCAACCACCCGGTGC
GCAACGAAATCACCAAGCGCGTCGAGATCACTTCGCACAATCACGGCTTCGCGGTGGATCCCGATTCGCTCAATCTCAAC
GAAGTGGAAATCACCCACGTGAACCTGAACGATCAGACGCTCGAAGGCTTCCGTCATCGCAGCCACCCGGTCTTCTGCGT
GCAATATCACCCGGAGGCCGCGCCCGGGCCGCACGATTCGCACTACCTATTCGACGATTTCACCAGGCTCATGCGGGAGG
CGAAATAA

Upstream 100 bases:

>100_bases
AAATTTGTCCCACTCGGGCAGGAGTGCCGGTGCGGCCCAACCCCGCGGTCCGGCAATGCTAAAATGAAGGTTATCCACGC
CCTGGGAGGAAAAACCTAAG

Downstream 100 bases:

>100_bases
CGATGCCGAGACGCAATGACATCCACCGCATCCTGATCATCGGCTCCGGCCCGATCATCATCGGCCAGGCCTGCGAGTTC
GATTACTCGGGCACGCAGGC

Product: carbamoyl-phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MNTAILALEDGSVFEGRSFGAPTERSGEVVFNTALTGYQEVFTDPSYSGQIVILTNPQIGNYGTSSRDNESKGPYIEGLV
VREFSSIASNWRSDSSADRFLANSGVPIVSDLDTRALVRHLRTRGVMRGVLSAVESDAQKLVEKARNIPTMTGLDLASRV
TTAERYEWTKTVEQCSPSDLVGKTAEPLYNVVAYDFGIKHNILRRLVHAGCRVTVVPALTSAEDVLALNPDGIFLSNGPG
DPEPLVPQVNNIRKLIGKKPIFGICLGNQLLGLAMGGKTYKLKFGHRGANHPVRNEITKRVEITSHNHGFAVDPDSLNLN
EVEITHVNLNDQTLEGFRHRSHPVFCVQYHPEAAPGPHDSHYLFDDFTRLMREAK

Sequences:

>Translated_375_residues
MNTAILALEDGSVFEGRSFGAPTERSGEVVFNTALTGYQEVFTDPSYSGQIVILTNPQIGNYGTSSRDNESKGPYIEGLV
VREFSSIASNWRSDSSADRFLANSGVPIVSDLDTRALVRHLRTRGVMRGVLSAVESDAQKLVEKARNIPTMTGLDLASRV
TTAERYEWTKTVEQCSPSDLVGKTAEPLYNVVAYDFGIKHNILRRLVHAGCRVTVVPALTSAEDVLALNPDGIFLSNGPG
DPEPLVPQVNNIRKLIGKKPIFGICLGNQLLGLAMGGKTYKLKFGHRGANHPVRNEITKRVEITSHNHGFAVDPDSLNLN
EVEITHVNLNDQTLEGFRHRSHPVFCVQYHPEAAPGPHDSHYLFDDFTRLMREAK
>Mature_375_residues
MNTAILALEDGSVFEGRSFGAPTERSGEVVFNTALTGYQEVFTDPSYSGQIVILTNPQIGNYGTSSRDNESKGPYIEGLV
VREFSSIASNWRSDSSADRFLANSGVPIVSDLDTRALVRHLRTRGVMRGVLSAVESDAQKLVEKARNIPTMTGLDLASRV
TTAERYEWTKTVEQCSPSDLVGKTAEPLYNVVAYDFGIKHNILRRLVHAGCRVTVVPALTSAEDVLALNPDGIFLSNGPG
DPEPLVPQVNNIRKLIGKKPIFGICLGNQLLGLAMGGKTYKLKFGHRGANHPVRNEITKRVEITSHNHGFAVDPDSLNLN
EVEITHVNLNDQTLEGFRHRSHPVFCVQYHPEAAPGPHDSHYLFDDFTRLMREAK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI21361331, Length=387, Percent_Identity=38.5012919896641, Blast_Score=248, Evalue=4e-66,
Organism=Homo sapiens, GI169790915, Length=387, Percent_Identity=38.5012919896641, Blast_Score=248, Evalue=4e-66,
Organism=Homo sapiens, GI18105007, Length=386, Percent_Identity=38.860103626943, Blast_Score=243, Evalue=2e-64,
Organism=Escherichia coli, GI1786215, Length=378, Percent_Identity=50.5291005291005, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1789760, Length=143, Percent_Identity=32.1678321678322, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=394, Percent_Identity=39.5939086294416, Blast_Score=250, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6324878, Length=379, Percent_Identity=40.8970976253298, Blast_Score=259, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6322331, Length=394, Percent_Identity=38.5786802030457, Blast_Score=252, Evalue=7e-68,
Organism=Drosophila melanogaster, GI45555749, Length=390, Percent_Identity=40.5128205128205, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24642586, Length=390, Percent_Identity=40.5128205128205, Blast_Score=243, Evalue=2e-64,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41234; Mature: 41234

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTAILALEDGSVFEGRSFGAPTERSGEVVFNTALTGYQEVFTDPSYSGQIVILTNPQIG
CCCEEEEEECCCEECCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCEEEEEECCCCC
NYGTSSRDNESKGPYIEGLVVREFSSIASNWRSDSSADRFLANSGVPIVSDLDTRALVRH
CCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEECCCHHHHHHH
LRTRGVMRGVLSAVESDAQKLVEKARNIPTMTGLDLASRVTTAERYEWTKTVEQCSPSDL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
VGKTAEPLYNVVAYDFGIKHNILRRLVHAGCRVTVVPALTSAEDVLALNPDGIFLSNGPG
CCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEECCCCC
DPEPLVPQVNNIRKLIGKKPIFGICLGNQLLGLAMGGKTYKLKFGHRGANHPVRNEITKR
CCCCCCCCHHHHHHHHCCCCEEEEEECCCEEEEEECCEEEEEEECCCCCCCCHHHHHHHE
VEITSHNHGFAVDPDSLNLNEVEITHVNLNDQTLEGFRHRSHPVFCVQYHPEAAPGPHDS
EEEEECCCCEEECCCCCCCCEEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
HYLFDDFTRLMREAK
CCHHHHHHHHHHHCC
>Mature Secondary Structure
MNTAILALEDGSVFEGRSFGAPTERSGEVVFNTALTGYQEVFTDPSYSGQIVILTNPQIG
CCCEEEEEECCCEECCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCEEEEEECCCCC
NYGTSSRDNESKGPYIEGLVVREFSSIASNWRSDSSADRFLANSGVPIVSDLDTRALVRH
CCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEECCCHHHHHHH
LRTRGVMRGVLSAVESDAQKLVEKARNIPTMTGLDLASRVTTAERYEWTKTVEQCSPSDL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
VGKTAEPLYNVVAYDFGIKHNILRRLVHAGCRVTVVPALTSAEDVLALNPDGIFLSNGPG
CCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEECCCCC
DPEPLVPQVNNIRKLIGKKPIFGICLGNQLLGLAMGGKTYKLKFGHRGANHPVRNEITKR
CCCCCCCCHHHHHHHHCCCCEEEEEECCCEEEEEECCEEEEEEECCCCCCCCHHHHHHHE
VEITSHNHGFAVDPDSLNLNEVEITHVNLNDQTLEGFRHRSHPVFCVQYHPEAAPGPHDS
EEEEECCCCEEECCCCCCCCEEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
HYLFDDFTRLMREAK
CCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA