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The map label for this gene is gacS [H]

Identifier: 116619668

GI number: 116619668

Start: 641100

End: 643289

Strand: Direct

Name: gacS [H]

Synonym: Acid_0531

Alternate gene names: 116619668

Gene position: 641100-643289 (Clockwise)

Preceding gene: 116619667

Following gene: 116619670

Centisome position: 6.43

GC content: 61.64

Gene sequence:

>2190_bases
ATGACTTTCGACCAGCCATCTCGCACCGACCAGTCCGACGAAGCCGCCACTATCCCGATTCTCACGGTCAGCATCCTCGA
GGAATCCGACGTCGTTGCCGCCCGGCAGCGAGCCCGCCAGATCGCCGCCGAGATTGGTTTCGACGTTCACCAGCAGACCC
GCCTTGCCACCGCCGTCTCCGAGATCGCCCGCAACGCCTACAATTACGGCGGCGGTGGCCGCGTCGAATTCTCCATCGAA
GGCACCACCGTTCCCCAGCTCTTCGTCATCCGCGTGGCGGACGCCGGCCGCGGCATCCCAGACCTGCAAAGCATTCTCGA
CGGCCGCTACCAGTCCGAAACCGGTATGGGACTCGGCATCTCCCGAGCCCGCCGCCTTATGGACCGCTTCGAAATCGAGT
CTCCGCCCGGCGGTGGCGTTACCGTCACCATGACCCGGATGCTGCCCCTCCGAGCCCCCCTCGTTACCCGGCTAACCCTG
GTCAAATTGCTCGACCGACTCGCCGCCCAGGCGCCGCCGGCCGCCCTCCAGGAGTTGCAGCAGCAGAATCGCGAACTGCT
CGCCACCCTGGAAGAACTTGCTGTCCGGCAGGAAGAATTGGTACGGCTTAACCTCGAACTGGAAGATACCAACCGCGGCG
TGGTGGCTCTTTATGCCGAACTCGACGATAAGGCCGATCACCTCCGCCGCGCCGACGAGATGAAGTCGCGCTTCCTCTCC
AACATGAGCCACGAGTTCCGCACTCCGCTTAATTCCATCCTGGCCCTGACCCGGCTGCTGCTCGATCGCGCCGAGGGCGA
TTTGACCGGCGAGCAGGAACGCCAGGTGTTTTTTATCAGGAAGTCCGCCGAAAGCCTGTACGAACTTGTCAACGACCTCC
TCGATCTCGCCAAAGTTCAGGCCGGCAAGACCACCGTCCACCTGGCTCCATTCGATGTGAAAAACCTCTTCGGCGCCCTT
CGCGGCATGCTCCGCCCCCTGCTTGTCAACACCGCGGTCAACCTGGTCTTCGAGGAGCCCGAAGGCATCTCCGAGGTGGT
CTCGGATGAAGGCAAAATCTCCCAGATCCTCAGAAACTTCATCTCGAATGCACTCAAATTTACCGACCACGGAGAAGTTC
GTGTTTTCGTCCGCCGCGAGGACGCCGATCGCGTCATCTTCTCCGTCTCCGATACCGGCATCGGAATCGATCCCGCCGAT
GCCGACCGTGTCTTTGAGGAATTCACCCAGTTGGAAAGCCCTATCCAACGTAAAGTAAAGGGCACCGGACTCGGGCTTCC
GCTCTCTCGCAAGCTCGCTGAGCTGCTTGGCGGCACCGTCAGCGTGGAGAGCAGTCCCGGCGTTGGCTCCACGTTTTCAT
TGACTATCCCAATCGGCGACGCCGTTTCAGCGGCCGCTCCGGCCGGTGAGTCCGCCTCTGAAATCGCCGGCAAACTGCCG
GTCCTGGTGGTGGAAGATGAGCCCGAAACCCGCATGATCTACGTAGACTACCTCAAAGGTAGTCAATACTACGTGATGCA
GGCGGCCACCCTGAACCAGGCCCGTGCCGCCATCGAGCGGGTTCGCCCCAGCGCGATCCTGCTTGATCTCATGCTCTCCA
ACGAGGATGGCTGCGCCCTTCTCGCCGAACTGAAAGGTGGCAAGGACACAAAGGATATCCCCGTGCTGGTCATAACTGTC
GTGGACGATCCCCACAAGGTATATGGACTGGGCGCGGACGTGTACGCCGTCAAACCAGTCAGTAAGAAATGGCTGCTCGA
TGCGCTGGATAAGGCGACCGGGCCCCCAGGGGCAGCGCGCCGCGTTCTTCTTGTTGACGACGACCCGACCTTCCGCTACC
TGGCCAGGCAGATCTTCGCCGGGATGCCGCATGAGTTCACCGAAGCCGATACCGGTGTCGCCGGCCTGGAAGCCGCTCGC
GCCACCCATCCCGAGCTCATCTTCCTCGACCTGATGATGCCGAAGATGTCAGGCTACGAAGTCCTCGAGCATTTGCGTGC
CGACCCCAAAACCCGCGATATTCCCGTGGTCGTGGTCAGTTCCCGCTTCCTCAACCCAGCCGAGCAGCGACAGCTCCGCG
ATCTGAACGCCGCCATTCTTCCCAAAGACGACTTCGCTCGCGCCGATCTCCTCTCCAACGTCCGCGAAATCCTCACCACC
TTCGGCCTCGCCAACCTCCTGCCCACGTAA

Upstream 100 bases:

>100_bases
GCATTATCGCCGGCATCTTATTCCGCGATTTCCAGCGCGGCACCGATGACGCCACGGTGGTGGTCGTGCGCTTCGCCGGC
GGCACTCAAGAGGACAGGCT

Downstream 100 bases:

>100_bases
CCCAACGAGCCGCGACCCGTCAGCGAGCGATCCGGTACACCCGAAACTCCCGAATCACCAGGTGACTGAATGTAGTCCGC
AGCCCGAACCTTCCGCGCCG

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MTFDQPSRTDQSDEAATIPILTVSILEESDVVAARQRARQIAAEIGFDVHQQTRLATAVSEIARNAYNYGGGGRVEFSIE
GTTVPQLFVIRVADAGRGIPDLQSILDGRYQSETGMGLGISRARRLMDRFEIESPPGGGVTVTMTRMLPLRAPLVTRLTL
VKLLDRLAAQAPPAALQELQQQNRELLATLEELAVRQEELVRLNLELEDTNRGVVALYAELDDKADHLRRADEMKSRFLS
NMSHEFRTPLNSILALTRLLLDRAEGDLTGEQERQVFFIRKSAESLYELVNDLLDLAKVQAGKTTVHLAPFDVKNLFGAL
RGMLRPLLVNTAVNLVFEEPEGISEVVSDEGKISQILRNFISNALKFTDHGEVRVFVRREDADRVIFSVSDTGIGIDPAD
ADRVFEEFTQLESPIQRKVKGTGLGLPLSRKLAELLGGTVSVESSPGVGSTFSLTIPIGDAVSAAAPAGESASEIAGKLP
VLVVEDEPETRMIYVDYLKGSQYYVMQAATLNQARAAIERVRPSAILLDLMLSNEDGCALLAELKGGKDTKDIPVLVITV
VDDPHKVYGLGADVYAVKPVSKKWLLDALDKATGPPGAARRVLLVDDDPTFRYLARQIFAGMPHEFTEADTGVAGLEAAR
ATHPELIFLDLMMPKMSGYEVLEHLRADPKTRDIPVVVVSSRFLNPAEQRQLRDLNAAILPKDDFARADLLSNVREILTT
FGLANLLPT

Sequences:

>Translated_729_residues
MTFDQPSRTDQSDEAATIPILTVSILEESDVVAARQRARQIAAEIGFDVHQQTRLATAVSEIARNAYNYGGGGRVEFSIE
GTTVPQLFVIRVADAGRGIPDLQSILDGRYQSETGMGLGISRARRLMDRFEIESPPGGGVTVTMTRMLPLRAPLVTRLTL
VKLLDRLAAQAPPAALQELQQQNRELLATLEELAVRQEELVRLNLELEDTNRGVVALYAELDDKADHLRRADEMKSRFLS
NMSHEFRTPLNSILALTRLLLDRAEGDLTGEQERQVFFIRKSAESLYELVNDLLDLAKVQAGKTTVHLAPFDVKNLFGAL
RGMLRPLLVNTAVNLVFEEPEGISEVVSDEGKISQILRNFISNALKFTDHGEVRVFVRREDADRVIFSVSDTGIGIDPAD
ADRVFEEFTQLESPIQRKVKGTGLGLPLSRKLAELLGGTVSVESSPGVGSTFSLTIPIGDAVSAAAPAGESASEIAGKLP
VLVVEDEPETRMIYVDYLKGSQYYVMQAATLNQARAAIERVRPSAILLDLMLSNEDGCALLAELKGGKDTKDIPVLVITV
VDDPHKVYGLGADVYAVKPVSKKWLLDALDKATGPPGAARRVLLVDDDPTFRYLARQIFAGMPHEFTEADTGVAGLEAAR
ATHPELIFLDLMMPKMSGYEVLEHLRADPKTRDIPVVVVSSRFLNPAEQRQLRDLNAAILPKDDFARADLLSNVREILTT
FGLANLLPT
>Mature_728_residues
TFDQPSRTDQSDEAATIPILTVSILEESDVVAARQRARQIAAEIGFDVHQQTRLATAVSEIARNAYNYGGGGRVEFSIEG
TTVPQLFVIRVADAGRGIPDLQSILDGRYQSETGMGLGISRARRLMDRFEIESPPGGGVTVTMTRMLPLRAPLVTRLTLV
KLLDRLAAQAPPAALQELQQQNRELLATLEELAVRQEELVRLNLELEDTNRGVVALYAELDDKADHLRRADEMKSRFLSN
MSHEFRTPLNSILALTRLLLDRAEGDLTGEQERQVFFIRKSAESLYELVNDLLDLAKVQAGKTTVHLAPFDVKNLFGALR
GMLRPLLVNTAVNLVFEEPEGISEVVSDEGKISQILRNFISNALKFTDHGEVRVFVRREDADRVIFSVSDTGIGIDPADA
DRVFEEFTQLESPIQRKVKGTGLGLPLSRKLAELLGGTVSVESSPGVGSTFSLTIPIGDAVSAAAPAGESASEIAGKLPV
LVVEDEPETRMIYVDYLKGSQYYVMQAATLNQARAAIERVRPSAILLDLMLSNEDGCALLAELKGGKDTKDIPVLVITVV
DDPHKVYGLGADVYAVKPVSKKWLLDALDKATGPPGAARRVLLVDDDPTFRYLARQIFAGMPHEFTEADTGVAGLEAARA
THPELIFLDLMMPKMSGYEVLEHLRADPKTRDIPVVVVSSRFLNPAEQRQLRDLNAAILPKDDFARADLLSNVREILTTF
GLANLLPT

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=484, Percent_Identity=28.7190082644628, Blast_Score=173, Evalue=4e-44,
Organism=Escherichia coli, GI48994928, Length=372, Percent_Identity=34.9462365591398, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI145693157, Length=491, Percent_Identity=27.0875763747454, Blast_Score=166, Evalue=7e-42,
Organism=Escherichia coli, GI87081816, Length=340, Percent_Identity=33.2352941176471, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1788713, Length=369, Percent_Identity=27.6422764227642, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1790436, Length=236, Percent_Identity=29.2372881355932, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1786912, Length=229, Percent_Identity=28.82096069869, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788393, Length=242, Percent_Identity=25.2066115702479, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI87082128, Length=238, Percent_Identity=28.1512605042017, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=28.1938325991189, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1790346, Length=265, Percent_Identity=28.3018867924528, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788549, Length=253, Percent_Identity=22.1343873517787, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1786783, Length=224, Percent_Identity=25.8928571428571, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787894, Length=252, Percent_Identity=24.2063492063492, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322044, Length=71, Percent_Identity=46.4788732394366, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 79901; Mature: 79770

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFDQPSRTDQSDEAATIPILTVSILEESDVVAARQRARQIAAEIGFDVHQQTRLATAVS
CCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EIARNAYNYGGGGRVEFSIEGTTVPQLFVIRVADAGRGIPDLQSILDGRYQSETGMGLGI
HHHHHHCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCH
SRARRLMDRFEIESPPGGGVTVTMTRMLPLRAPLVTRLTLVKLLDRLAAQAPPAALQELQ
HHHHHHHHHHCCCCCCCCCEEEEEEEHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QQNRELLATLEELAVRQEELVRLNLELEDTNRGVVALYAELDDKADHLRRADEMKSRFLS
HHHHHHHHHHHHHHHCHHHHEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
NMSHEFRTPLNSILALTRLLLDRAEGDLTGEQERQVFFIRKSAESLYELVNDLLDLAKVQ
HHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHC
AGKTTVHLAPFDVKNLFGALRGMLRPLLVNTAVNLVFEEPEGISEVVSDEGKISQILRNF
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHCCCCHHHHHHHHH
ISNALKFTDHGEVRVFVRREDADRVIFSVSDTGIGIDPADADRVFEEFTQLESPIQRKVK
HHHHHEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GTGLGLPLSRKLAELLGGTVSVESSPGVGSTFSLTIPIGDAVSAAAPAGESASEIAGKLP
CCCCCCCHHHHHHHHHCCEEEECCCCCCCCEEEEEEECCCCHHHCCCCCCCHHHHHCCCC
VLVVEDEPETRMIYVDYLKGSQYYVMQAATLNQARAAIERVRPSAILLDLMLSNEDGCAL
EEEECCCCCCEEEEEEEECCCEEEEEEHHHHHHHHHHHHHHCCHHEEEEHEECCCCCCEE
LAELKGGKDTKDIPVLVITVVDDPHKVYGLGADVYAVKPVSKKWLLDALDKATGPPGAAR
EEECCCCCCCCCCCEEEEEEECCCCEEEECCCCEEEECCCCHHHHHHHHHHCCCCCCHHE
RVLLVDDDPTFRYLARQIFAGMPHEFTEADTGVAGLEAARATHPELIFLDLMMPKMSGYE
EEEEECCCCHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHCCCCCEEEEEECCCCCCHHH
VLEHLRADPKTRDIPVVVVSSRFLNPAEQRQLRDLNAAILPKDDFARADLLSNVREILTT
HHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHH
FGLANLLPT
HHHHHHCCC
>Mature Secondary Structure 
TFDQPSRTDQSDEAATIPILTVSILEESDVVAARQRARQIAAEIGFDVHQQTRLATAVS
CCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EIARNAYNYGGGGRVEFSIEGTTVPQLFVIRVADAGRGIPDLQSILDGRYQSETGMGLGI
HHHHHHCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCH
SRARRLMDRFEIESPPGGGVTVTMTRMLPLRAPLVTRLTLVKLLDRLAAQAPPAALQELQ
HHHHHHHHHHCCCCCCCCCEEEEEEEHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QQNRELLATLEELAVRQEELVRLNLELEDTNRGVVALYAELDDKADHLRRADEMKSRFLS
HHHHHHHHHHHHHHHCHHHHEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
NMSHEFRTPLNSILALTRLLLDRAEGDLTGEQERQVFFIRKSAESLYELVNDLLDLAKVQ
HHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHC
AGKTTVHLAPFDVKNLFGALRGMLRPLLVNTAVNLVFEEPEGISEVVSDEGKISQILRNF
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHCCCCHHHHHHHHH
ISNALKFTDHGEVRVFVRREDADRVIFSVSDTGIGIDPADADRVFEEFTQLESPIQRKVK
HHHHHEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GTGLGLPLSRKLAELLGGTVSVESSPGVGSTFSLTIPIGDAVSAAAPAGESASEIAGKLP
CCCCCCCHHHHHHHHHCCEEEECCCCCCCCEEEEEEECCCCHHHCCCCCCCHHHHHCCCC
VLVVEDEPETRMIYVDYLKGSQYYVMQAATLNQARAAIERVRPSAILLDLMLSNEDGCAL
EEEECCCCCCEEEEEEEECCCEEEEEEHHHHHHHHHHHHHHCCHHEEEEHEECCCCCCEE
LAELKGGKDTKDIPVLVITVVDDPHKVYGLGADVYAVKPVSKKWLLDALDKATGPPGAAR
EEECCCCCCCCCCCEEEEEEECCCCEEEECCCCEEEECCCCHHHHHHHHHHCCCCCCHHE
RVLLVDDDPTFRYLARQIFAGMPHEFTEADTGVAGLEAARATHPELIFLDLMMPKMSGYE
EEEEECCCCHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHCCCCCEEEEEECCCCCCHHH
VLEHLRADPKTRDIPVVVVSSRFLNPAEQRQLRDLNAAILPKDDFARADLLSNVREILTT
HHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHH
FGLANLLPT
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]