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The map label for this gene is hemK [H]

Identifier: 116517180

GI number: 116517180

Start: 918896

End: 919735

Strand: Direct

Name: hemK [H]

Synonym: SPD_0907

Alternate gene names: 116517180

Gene position: 918896-919735 (Clockwise)

Preceding gene: 116517153

Following gene: 116516461

Centisome position: 44.91

GC content: 37.98

Gene sequence:

>840_bases
ATGAAATTAGCTCAATTATTTTCAAATTTTGAAGAAGAGTTGATAAGACAAGGAGAGGAAGCGGAAAGCCTCTCTTTTGT
CTATCGTAGTCTGAAAAATCTATCTTTTACAGACTTTATTTTTGCCCTCCAGCAGGAGGTGACAACAGAGGAAGAAAAAC
AATTTGTAGAAGATATTTACCAGCAACTAGCAGCTCACAAGCCAGCTCAGTACATCATTGGACAGGCAGATTTTTATGGA
ATGCATTTAAAGGTGGATGAACGGGTTTTGATTCCTCGTCCAGAAACAGAGGAGTTAGTGGAGCTTATTCTAACTGAAAA
TCTTGAGACGAATCTCTCAGTTCTGGATATTGGAACAGGTAGTGGAGCTATTGCTCTCGCTCTAGCAAAAAATAGACCAG
ATTGGTCAGTGACAGCAGCAGATATTTCTCAAGAGGCCTTAGATTTAGCTAGAGAAAATGCTAAAAATCAAAATCTTCAA
ATATTTTTAAAAAAATCTGACTGTTTTACAGAAATTTCTGAAAAATATGATATAATTGTTTCCAATCCACCCTATATCTC
TCGTGAAGATGAGTCAGAGGTAGGCTTGAATGTCTTGTATTCGGAGCCTCATCTAGCTCTCTTTGCAGACGAGGATGGCC
TAGCTATTTACCGTAGAATTGCGGAAGATGCAACAGACTATCTCAAAGATAGTGGTAAGATTTACCTTGAAATTGGATAC
AAGCAAGGTCAATGTGTTCCCGAACTTTTTAGAAAACATCTTCCTGAAAAAAGAGTTCGAACACTCAAAGACCAATTTGG
TCAAAATAGGATGGTTGTGGTTGATGATGGACAGGATTAG

Upstream 100 bases:

>100_bases
TCCAAAAACTAGATACGATTTTGTCTGGTAAATTGGACGAAGTTGTGGATGCCTTGGTGCTTTATGACCAAACACAAAAA
CTAGAAGAATTAAACAAATA

Downstream 100 bases:

>100_bases
ACAAGAGTTGGAAAAGGGTGGAGCTGTCGTTCTACCTACAGAGACTGTTTATGGTCTTTTTTCCAAGGCCTTAGATGAAA
AAGCAGTTGACCATGTTTAC

Product: HemK protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQQEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYG
MHLKVDERVLIPRPETEELVELILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADISQEALDLARENAKNQNLQ
IFLKKSDCFTEISEKYDIIVSNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYRRIAEDATDYLKDSGKIYLEIGY
KQGQCVPELFRKHLPEKRVRTLKDQFGQNRMVVVDDGQD

Sequences:

>Translated_279_residues
MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQQEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYG
MHLKVDERVLIPRPETEELVELILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADISQEALDLARENAKNQNLQ
IFLKKSDCFTEISEKYDIIVSNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYRRIAEDATDYLKDSGKIYLEIGY
KQGQCVPELFRKHLPEKRVRTLKDQFGQNRMVVVDDGQD
>Mature_279_residues
MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQQEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYG
MHLKVDERVLIPRPETEELVELILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADISQEALDLARENAKNQNLQ
IFLKKSDCFTEISEKYDIIVSNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYRRIAEDATDYLKDSGKIYLEIGY
KQGQCVPELFRKHLPEKRVRTLKDQFGQNRMVVVDDGQD

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=253, Percent_Identity=33.9920948616601, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1787463, Length=214, Percent_Identity=36.4485981308411, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87082085, Length=197, Percent_Identity=37.5634517766497, Blast_Score=122, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6324266, Length=163, Percent_Identity=33.7423312883436, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24582226, Length=189, Percent_Identity=34.9206349206349, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31851; Mature: 31851

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQQEVTTEEEKQFVEDIY
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHH
QQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVELILTENLETNLSVLDIGTG
HHHHCCCCHHHEECCCCEEEEEEEECCEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCC
SGAIALALAKNRPDWSVTAADISQEALDLARENAKNQNLQIFLKKSDCFTEISEKYDIIV
CCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHCCCEEEE
SNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYRRIAEDATDYLKDSGKIYLEIGY
CCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECC
KQGQCVPELFRKHLPEKRVRTLKDQFGQNRMVVVDDGQD
CCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCC
>Mature Secondary Structure
MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQQEVTTEEEKQFVEDIY
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHH
QQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVELILTENLETNLSVLDIGTG
HHHHCCCCHHHEECCCCEEEEEEEECCEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCC
SGAIALALAKNRPDWSVTAADISQEALDLARENAKNQNLQIFLKKSDCFTEISEKYDIIV
CCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHCCCEEEE
SNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYRRIAEDATDYLKDSGKIYLEIGY
CCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECC
KQGQCVPELFRKHLPEKRVRTLKDQFGQNRMVVVDDGQD
CCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]