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The map label for this gene is pheT [H]

Identifier: 116517104

GI number: 116517104

Start: 514749

End: 517151

Strand: Direct

Name: pheT [H]

Synonym: SPD_0506

Alternate gene names: 116517104

Gene position: 514749-517151 (Clockwise)

Preceding gene: 116516224

Following gene: 116515852

Centisome position: 25.16

GC content: 45.82

Gene sequence:

>2403_bases
ATGCTTATATCTTATAAATGGTTAAAAGAATTGGTGGACATTGATGTGCCATCACAAGAGTTGGCTGAAAAAATGTCAAC
TACAGGAATCGAGGTAGAGGGTGTCGAATCACCAGCTGCTGGTCTCTCAAAAATTGTCGTCGGTGAGGTCTTGTCTTGCG
AAGATGTGCCAGAGACTCACCTCCATGTTTGTCAGGTTAACGTTGGCGAAGAAGAGCGTCAGATCGTTTGTGGTGCCCCA
AATGTGCGTGCTGGGATCAAGGTCATGGTGGCTCTTCCAGGAGCTCGTATCGCTGATAACTACAAAATCAAAAAAGGAAA
AATCCGTGGTTTGGAGTCACTTGGAATGATCTGTTCACTTGGTGAATTGGGAATTTCTGACTCAGTTGTGCCTAAGGAAT
TCGCAGATGGCATCCAAATCTTGCCTGAAGATGCCGTGCCAGGTGAGGAAGTCTTTTCTTACCTAGACTTGGATGATGAA
ATCATCGAACTTTCCATCACACCAAACCGTGCAGATGCCCTTTCTATGTGTGGAGTGGCTCACGAAGTGGCAGCCATCTA
TGACAAGGCAGTCAACTTTAAAGAATTTACTCTAACAGAAACTAATGAAGCTGCGGCAGATGCCCTTTCTGTCAGCATTG
AGACAGACAAGGCGCCTTACTATGCAGCTCGTATCTTGGACAATGTGACCATCGCACCAAGTCCACAATGGTTGCAAAAC
CTTCTCATGAACGAAGGAATCCGTCCCATCAATAACGTAGTGGACGTGACCAACTACATCCTGCTCTATTTTGGTCAACC
AATGCATGCCTTTGACTTGGATAACTTTGAAGGGACTGACATCCGTGTGCGTGAAGCGCGTGCTGGTGAAAAATTGGTGA
CCTTGGACGGTGAAGAACGTGACTTGGACGTGAATGACCTAGTCATCACTGTCGCAGACAAGCCAGTAGCCCTTGCAGGT
GTCATGGGTGGTCAAGCAACAGAAATCTCTGAAAAATCTAGTCGTGTTGTCCTTGAAGCTGCTGTTTTCAATGGCAAATC
TATCCGTAAGACAAGTGGTCGCCTGAACCTTCGTTCTGAGTCATCTTCTCGCTTTGAAAAAGGAATTAATGTGGCAACAG
TTAATGAAGCCCTTGATGCGGCAGCTAGCCTGATTGCGGAACTTGCAGGTGCGACGGTGCGTAAGGGCATCGTTTCAGCG
GGTGAGCTTGATACTTCAGATGTAGAAGTTTCTTCAACCCTTGCTGATGTTAACCGTGTCCTCGGAACTGAGCTGTCTTA
TGCTGATGTAGAAGACGTCTTCCGTCGTCTTGGCTTTGGTCTTTCTGGAAATGCAGACAGCTTTACAGTCAGAGTCCCAC
GTCGTCGTTGGGATATCACAATCGAAGCTGACCTCTTTGAAGAAATTGCTCGTATCTATGGTTATGACCGCTTGCCAACT
AGTCTACCAAAAGACGATGGTACAGCAGGTGAATTGACAGCCACACAAAAACTCCGCCGTCAAGTTCGTACTATTGCTGA
AGGAGCAGGTTTGACAGAAATCATCACCTATACTCTAACAACTCCTGAAAAAGCAGTTGAGTTTACGGCTCAACCAAGTA
ACCTTACGGAACTCATGTGGCCAATGACTGTGGATCGTTCAGTCCTCCGTCAAAATATGATTTCAGGTATCCTTGATACC
GTTGCCTACAACGTGGCTCGTAAGAATAAAAACTTGGCCCTTTACGAGATTGGAAAAGTCTTTGAACAAACAGGTAATCC
AAAAGAAGAACTTCCAAATGAAATCAACAGTTTTGCCTTTGCCTTGACAGGCTTGGTTGCTGAAAAAGATTTCCAAACAG
CAGCAGTTCCAGTTGATTTCTTCTATGCTAAGGGAATCCTTGAAGCCCTATTTACTCGTTTGGGACTCCAAGTAACCTAT
ACAGCAACATCTGAAATCGCTAGCCTTCATCCAGGTCGTACAGCCGTGATTTCACTCGGTGACCAAGTTCTTGGTTTCCT
TGGCCAAGTGCATCCAGTCACTGCCAAGGCTTACGATATTCCAGAAACGTATGTGGCTGAGCTTAACCTTTCAGCTATCG
AAGCTGCGCTTCAGCCAGCGACTCCATTTGTAGAAATCACCAAATTCCCGGCAGTCAGCCGTGACGTTGCCCTTCTCCTC
AAGGCAGAAGTGACTCATCAAGAAGTTGTAGATGCTATCCAAGCTGCCGGCGTGAAACGTTTGACAGATATCAAACTCTT
TGACGTCTTCTCAGGTGAGAAATTGGGACTTGGTATGAAGTCAATGGCTTATAGCTTGACCTTCCAAAATCCAGAAGATA
GCTTAACGGACGAAGAAGTCGCACGCTATATGGAAAAAATCCAAGCATCGCTCGAAGAAAAAGTCAATGCAGAAGTGCGT
TAA

Upstream 100 bases:

>100_bases
TTACTGAGAAAGAAATTAAGGTAAGAAGAGGATTCTTCTATTGAAATGATAGGAAAGGAAAAGAACCAGTGTGGCAATGG
TCATTCTGGAGAACTAAATT

Downstream 100 bases:

>100_bases
ACGATAGTATGATACAAATTCCCCCTCTAGTTTATGAACACTAGAGGGGTGATTTTATATAAAATAATTAAAATGTGATT
TCTGTAAAAATACATCTATG

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 800; Mature: 800

Protein sequence:

>800_residues
MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSKIVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAP
NVRAGIKVMVALPGARIADNYKIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKAPYYAARILDNVTIAPSPQWLQN
LLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNFEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAG
VMGGQATEISEKSSRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGATVRKGIVSA
GELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTVRVPRRRWDITIEADLFEEIARIYGYDRLPT
SLPKDDGTAGELTATQKLRRQVRTIAEGAGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDT
VAYNVARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAKGILEALFTRLGLQVTY
TATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIPETYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLL
KAEVTHQEVVDAIQAAGVKRLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAEVR

Sequences:

>Translated_800_residues
MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSKIVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAP
NVRAGIKVMVALPGARIADNYKIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKAPYYAARILDNVTIAPSPQWLQN
LLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNFEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAG
VMGGQATEISEKSSRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGATVRKGIVSA
GELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTVRVPRRRWDITIEADLFEEIARIYGYDRLPT
SLPKDDGTAGELTATQKLRRQVRTIAEGAGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDT
VAYNVARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAKGILEALFTRLGLQVTY
TATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIPETYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLL
KAEVTHQEVVDAIQAAGVKRLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAEVR
>Mature_800_residues
MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSKIVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAP
NVRAGIKVMVALPGARIADNYKIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKAPYYAARILDNVTIAPSPQWLQN
LLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNFEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAG
VMGGQATEISEKSSRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGATVRKGIVSA
GELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTVRVPRRRWDITIEADLFEEIARIYGYDRLPT
SLPKDDGTAGELTATQKLRRQVRTIAEGAGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDT
VAYNVARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAKGILEALFTRLGLQVTY
TATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIPETYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLL
KAEVTHQEVVDAIQAAGVKRLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAEVR

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=813, Percent_Identity=35.1783517835178, Blast_Score=422, Evalue=1e-119,
Organism=Drosophila melanogaster, GI21355211, Length=316, Percent_Identity=24.0506329113924, Blast_Score=76, Evalue=1e-13,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 87041; Mature: 87041

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSKIVVGEVLSCEDVPETH
CEECHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCC
LHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYKIKKGKIRGLESLGMICSL
EEEEEEECCCCCCEEEECCCCCCCCEEEEEECCCCEECCCCEEECCCCCCHHHHHHHHHH
GELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDEIIELSITPNRADALSMCGVA
CCCCCCCCCCCHHHHCCCEECCCCCCCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
HEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKAPYYAARILDNVTIAPSPQWLQN
HHHHHHHHHHCCCEEEEEECCCHHHHHEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHH
LLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNFEGTDIRVREARAGEKLVTLDGEER
HHHHCCCCHHHHHHHHHHHEEEEECCCCEEECCCCCCCCCEEEEECCCCCEEEEECCCCC
DLDVNDLVITVADKPVALAGVMGGQATEISEKSSRVVLEAAVFNGKSIRKTSGRLNLRSE
CCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCEEEEEEEEECCCCEEECCCCEEECCC
SSSRFEKGINVATVNEALDAAASLIAELAGATVRKGIVSAGELDTSDVEVSSTLADVNRV
CHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LGTELSYADVEDVFRRLGFGLSGNADSFTVRVPRRRWDITIEADLFEEIARIYGYDRLPT
HCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEEHHHHHHHHHHHCCCCCCC
SLPKDDGTAGELTATQKLRRQVRTIAEGAGLTEIITYTLTTPEKAVEFTAQPSNLTELMW
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHEEECCCCCHHHHHC
PMTVDRSVLRQNMISGILDTVAYNVARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAF
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHH
ALTGLVAEKDFQTAAVPVDFFYAKGILEALFTRLGLQVTYTATSEIASLHPGRTAVISLG
HHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCCCCEEEEECC
DQVLGFLGQVHPVTAKAYDIPETYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLL
HHHHHHHCCCCCCEEEECCCCHHHHHHCCHHHHHHHHCCCCCCEEEECCCCCCCCHHHHH
KAEVTHQEVVDAIQAAGVKRLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEV
HHHCHHHHHHHHHHHCCCHHHHCEEEEEECCCCCCCCCHHHHEEEEEECCCCCCCCHHHH
ARYMEKIQASLEEKVNAEVR
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSKIVVGEVLSCEDVPETH
CEECHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCC
LHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYKIKKGKIRGLESLGMICSL
EEEEEEECCCCCCEEEECCCCCCCCEEEEEECCCCEECCCCEEECCCCCCHHHHHHHHHH
GELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDEIIELSITPNRADALSMCGVA
CCCCCCCCCCCHHHHCCCEECCCCCCCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
HEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKAPYYAARILDNVTIAPSPQWLQN
HHHHHHHHHHCCCEEEEEECCCHHHHHEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHH
LLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNFEGTDIRVREARAGEKLVTLDGEER
HHHHCCCCHHHHHHHHHHHEEEEECCCCEEECCCCCCCCCEEEEECCCCCEEEEECCCCC
DLDVNDLVITVADKPVALAGVMGGQATEISEKSSRVVLEAAVFNGKSIRKTSGRLNLRSE
CCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCEEEEEEEEECCCCEEECCCCEEECCC
SSSRFEKGINVATVNEALDAAASLIAELAGATVRKGIVSAGELDTSDVEVSSTLADVNRV
CHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LGTELSYADVEDVFRRLGFGLSGNADSFTVRVPRRRWDITIEADLFEEIARIYGYDRLPT
HCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEEHHHHHHHHHHHCCCCCCC
SLPKDDGTAGELTATQKLRRQVRTIAEGAGLTEIITYTLTTPEKAVEFTAQPSNLTELMW
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHEEECCCCCHHHHHC
PMTVDRSVLRQNMISGILDTVAYNVARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAF
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHH
ALTGLVAEKDFQTAAVPVDFFYAKGILEALFTRLGLQVTYTATSEIASLHPGRTAVISLG
HHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCCCCEEEEECC
DQVLGFLGQVHPVTAKAYDIPETYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLL
HHHHHHHCCCCCCEEEECCCCHHHHHHCCHHHHHHHHCCCCCCEEEECCCCCCCCHHHHH
KAEVTHQEVVDAIQAAGVKRLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEV
HHHCHHHHHHHHHHHCCCHHHHCEEEEEECCCCCCCCCHHHHEEEEEECCCCCCCCHHHH
ARYMEKIQASLEEKVNAEVR
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]