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The map label for this gene is glyA

Identifier: 116517005

GI number: 116517005

Start: 920816

End: 922072

Strand: Direct

Name: glyA

Synonym: SPD_0910

Alternate gene names: 116517005

Gene position: 920816-922072 (Clockwise)

Preceding gene: 116515388

Following gene: 116516178

Centisome position: 45.0

GC content: 42.56

Gene sequence:

>1257_bases
ATGATTTTTGACAAAGATGATTTTAAAGCATATGATGCTGATCTCTGGAATGCTATTGCCAAAGAAGAAGAACGCCAACA
AAATAATATCGAGTTAATTGCTTCGGAAAACGTAGTTTCCAAGGCTGTTATGGCAGCTCAAGGGTCTATCTTGACAAATA
AATATGCCGAGGGTTACCCAGGACGCCGTTATTATGGTGGAACTGATGTAGTAGACGTTGTAGAGACTCTTGCTATTGAA
CGCGCAAAAGAAATTTTCGGTGCTAAATTTGCTAATGTCCAACCACATTCAGGAAGCCAAGCTAACTGTGCGGCTTACAT
GTCCTTGATTGAGCCAGGTGATACGGTTATGGGAATGGATTTGGCAGCAGGTGGACACTTGACCCACGGAGCTCCAGTTA
GCTTCTCTGGTCAAACCTACAACTTTCTTTCCTATAGTGTGGATCCTGAAACGGAACTTTTGGACTTTGATGCTATCTTG
AAACAAGCCCAAGAAGTAAAACCAAAACTGATTGTAGCTGGTGCTTCAGCATATTCTCAAATTATCGATTTTTCAAAATT
CCGTGAAATCGCAGATGCTGTCGGTGCGAAGCTCATGGTGGACATGGCCCATATCGCTGGTTTGGTTGCGGCTGGCCTTC
ATCCAAGCCCAGTTCCATACGCTCATATCACAACAACAACGACCCACAAAACCCTTCGTGGACCTCGTGGTGGTTTGATT
TTGACCAATGACGAAGAACTTGCTAAAAAAATCAATTCAGCTATTTTCCCAGGTATTCAGGGCGGTCCTTTAGAGCATGT
TGTGGCGGCTAAGGCAGTTTCCTTCAAAGAAGTTTTGGATCCAGCCTTCAAGGAATATGCTGCCAATGTAATTAAGAACA
GCAAGGCTATGGCAGATGTCTTCTTGCAAGACCCTGATTTCCGTATTATTTCAGGTGGAACTGAAAACCATCTCTTCCTT
GTTGATGTGACTAAGGTTGTAGAAAACGGAAAAGTTGCTCAAAACTTGCTGGATGAAGTCAATATTACCCTAAATAAAAA
TTCAATCCCTTACGAAACCTTGTCGCCATTCAAGACAAGTGGGATTCGTATCGGAGCAGCAGCTATTACTGCACGTGGAT
TTGGTGAAGAAGAAAGTCGCAAAGTGGCTGAACTCATCATTAAAACCCTTAAGAATTCAGAAAATGAGGCTGTATTAGAA
GAAGTGAGAAGTGCAGTCAAAGAATTGACAGATGCCTTCCCATTATACGAGGACTAA

Upstream 100 bases:

>100_bases
TGTGATAAAATGCAGAAACGGTTTATTCGCATCTTTTAGTTTGATTTTCTTATTGTAAAATCAAACTAATGGACTAGTCA
CACAATAAAGGAGAAGACCT

Downstream 100 bases:

>100_bases
AACTTTTATGGATATTTATATTAAGAAAGCCATTATTCACCAGTTCAGTCCGGATGATACCGAGCTGTTCTTAGCAGATA
AGTTTCTCAATATTACTCCA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVMAAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIE
RAKEIFGAKFANVQPHSGSQANCAAYMSLIEPGDTVMGMDLAAGGHLTHGAPVSFSGQTYNFLSYSVDPETELLDFDAIL
KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPVPYAHITTTTTHKTLRGPRGGLI
LTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFKEVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFL
VDVTKVVENGKVAQNLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKNSENEAVLE
EVRSAVKELTDAFPLYED

Sequences:

>Translated_418_residues
MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVMAAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIE
RAKEIFGAKFANVQPHSGSQANCAAYMSLIEPGDTVMGMDLAAGGHLTHGAPVSFSGQTYNFLSYSVDPETELLDFDAIL
KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPVPYAHITTTTTHKTLRGPRGGLI
LTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFKEVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFL
VDVTKVVENGKVAQNLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKNSENEAVLE
EVRSAVKELTDAFPLYED
>Mature_418_residues
MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVMAAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIE
RAKEIFGAKFANVQPHSGSQANCAAYMSLIEPGDTVMGMDLAAGGHLTHGAPVSFSGQTYNFLSYSVDPETELLDFDAIL
KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPVPYAHITTTTTHKTLRGPRGGLI
LTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFKEVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFL
VDVTKVVENGKVAQNLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKNSENEAVLE
EVRSAVKELTDAFPLYED

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=448, Percent_Identity=42.4107142857143, Blast_Score=358, Evalue=7e-99,
Organism=Homo sapiens, GI261862352, Length=448, Percent_Identity=42.4107142857143, Blast_Score=357, Evalue=8e-99,
Organism=Homo sapiens, GI261862350, Length=448, Percent_Identity=42.4107142857143, Blast_Score=357, Evalue=8e-99,
Organism=Homo sapiens, GI261862348, Length=448, Percent_Identity=42.4107142857143, Blast_Score=357, Evalue=8e-99,
Organism=Homo sapiens, GI261862346, Length=443, Percent_Identity=42.2121896162528, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI22547186, Length=427, Percent_Identity=42.1545667447307, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI22547189, Length=413, Percent_Identity=39.9515738498789, Blast_Score=282, Evalue=3e-76,
Organism=Escherichia coli, GI1788902, Length=408, Percent_Identity=58.8235294117647, Blast_Score=476, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI25144732, Length=426, Percent_Identity=43.8967136150235, Blast_Score=347, Evalue=6e-96,
Organism=Caenorhabditis elegans, GI25144729, Length=426, Percent_Identity=43.8967136150235, Blast_Score=347, Evalue=9e-96,
Organism=Saccharomyces cerevisiae, GI6319739, Length=426, Percent_Identity=43.8967136150235, Blast_Score=352, Evalue=7e-98,
Organism=Saccharomyces cerevisiae, GI6323087, Length=403, Percent_Identity=43.6724565756824, Blast_Score=325, Evalue=9e-90,
Organism=Drosophila melanogaster, GI24640005, Length=449, Percent_Identity=42.5389755011136, Blast_Score=357, Evalue=8e-99,
Organism=Drosophila melanogaster, GI221329721, Length=449, Percent_Identity=42.5389755011136, Blast_Score=357, Evalue=1e-98,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_STRP2 (Q04KR0)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816388.1
- ProteinModelPortal:   Q04KR0
- STRING:   Q04KR0
- EnsemblBacteria:   EBSTRT00000019709
- GeneID:   4441989
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0910
- eggNOG:   COG0112
- GeneTree:   EBGT00050000027108
- HOGENOM:   HBG301263
- OMA:   ADVEANV
- ProtClustDB:   PRK00011
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45257; Mature: 45257

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVMAAQGSILTNKYAEGYP
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCHHHHHHHCCCC
GRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQANCAAYMSLIEPGDTVMGMD
CCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEE
LAAGGHLTHGAPVSFSGQTYNFLSYSVDPETELLDFDAILKQAQEVKPKLIVAGASAYSQ
ECCCCCCCCCCCCEECCCEEEEEEEECCCCHHHCCHHHHHHHHHHCCCEEEEECCHHHHH
IIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPVPYAHITTTTTHKTLRGPRGGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHCCCCCCEE
LTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFKEVLDPAFKEYAANVIKNSKAMADV
EECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQNLLDEVNITLNKNSIPYETLSPFKTS
HCCCCCEEEEECCCCCCEEEEEHHHHHCCCHHHHHHHHHCEEEECCCCCCCHHCCCCCCC
GIRIGAAAITARGFGEEESRKVAELIIKTLKNSENEAVLEEVRSAVKELTDAFPLYED
CEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVMAAQGSILTNKYAEGYP
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCHHHHHHHCCCC
GRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQANCAAYMSLIEPGDTVMGMD
CCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEE
LAAGGHLTHGAPVSFSGQTYNFLSYSVDPETELLDFDAILKQAQEVKPKLIVAGASAYSQ
ECCCCCCCCCCCCEECCCEEEEEEEECCCCHHHCCHHHHHHHHHHCCCEEEEECCHHHHH
IIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPVPYAHITTTTTHKTLRGPRGGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHCCCCCCEE
LTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFKEVLDPAFKEYAANVIKNSKAMADV
EECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQNLLDEVNITLNKNSIPYETLSPFKTS
HCCCCCEEEEECCCCCCEEEEEHHHHHCCCHHHHHHHHHCEEEECCCCCCCHHCCCCCCC
GIRIGAAAITARGFGEEESRKVAELIIKTLKNSENEAVLEEVRSAVKELTDAFPLYED
CEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA