Click here to switch to the map view.

The map label for this gene is wcaJ [H]

Identifier: 116516993

GI number: 116516993

Start: 1635156

End: 1635848

Strand: Reverse

Name: wcaJ [H]

Synonym: SPD_1620

Alternate gene names: 116516993

Gene position: 1635848-1635156 (Counterclockwise)

Preceding gene: 116516065

Following gene: 116516120

Centisome position: 79.95

GC content: 45.6

Gene sequence:

>693_bases
ATGCTGAAATGGGAAGACTTGCCTGTGGAAATGAAATCAAGCGAGGTTGAGTCTTACTACCAGCTTGTCTCTAAAAGGAA
GGGTTCGCTGATTTTCAAGCGTTGCTTGGACTGGGTTTTGGCCTTGGTCTTACTGGTTCTAACTTCTCCCATCTTTCTCA
TCTTGAGCATTTGGATCAAGTTGGATAGCAAGGGGCCAGTGATTTACAAGCAAGAGCGTGTGACCCAGTACAACCGTCGG
TTCAAGATTTGGAAGTTCCGTACCATGGTGACGGATGCGGATAAAAAAGGAAGTCTGGTGACTTCTGCTAACGATAGCCG
TATTACCAAGGTTGGAAATTTCATCCGACGTGTCCGTTTGGACGAACTGCCTCAATTGGTCAATGTCCTTAAAGGTGAGA
TGTCCTTTGTCGGTACACGACCTGAAGTGCCACGTTATACAGAGCAGTATAGCCCTGAAATGATGGCAACCTTGCTCTTG
CAAGCAGGAATTACCTCTCCAGCCAGCATCAACTACAAGGATGAGGACACCATCATCAGTCAAATGACGGAGAAAGGTCT
GTCAGTTGATCAGGCCTATGTGGAGCATGTTCTTCCTGAAAAGATGCGCTATAACCTCGCCTATCTCCGAGAGTTTAGTT
TCTTTGGGGACATCAAAATCATGTTTCAAACCGTGTTTGAGGTACTAAAATAA

Upstream 100 bases:

>100_bases
GGAAATCCTTCAAATTACAGATTTCTTTCACCGCCTTTTCCATTTTGTGGTATAATGAAAAATGTTGACAAATAGTATAA
TAAAAACAAAGGAGAACAGC

Downstream 100 bases:

>100_bases
AGTAGTCATAAGAAAATGAGTACAGATAAAAGGAGCAAATCAATGCCAAATTACAATATTCCATTTTCACCGCCTGATAT
CACAGAAGCAGAAATTGCTG

Product: glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVLLVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRR
FKIWKFRTMVTDADKKGSLVTSANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDIKIMFQTVFEVLK

Sequences:

>Translated_230_residues
MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVLLVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRR
FKIWKFRTMVTDADKKGSLVTSANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDIKIMFQTVFEVLK
>Mature_230_residues
MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVLLVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRR
FKIWKFRTMVTDADKKGSLVTSANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDIKIMFQTVFEVLK

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=201, Percent_Identity=38.8059701492537, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 26786; Mature: 26786

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVLLVLTSPIFLILSIWIK
CCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTSANDSRITKVGNFIRRVRL
CCCCCCEEEHHHHHHHHHHHHHHEEHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHH
DELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLLQAGITSPASINYKDEDTIIS
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
QMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDIKIMFQTVFEVLK
HHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVLLVLTSPIFLILSIWIK
CCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTSANDSRITKVGNFIRRVRL
CCCCCCEEEHHHHHHHHHHHHHHEEHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHH
DELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLLQAGITSPASINYKDEDTIIS
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
QMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDIKIMFQTVFEVLK
HHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]