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The map label for this gene is hinfIM [H]

Identifier: 116516980

GI number: 116516980

Start: 1288160

End: 1289338

Strand: Direct

Name: hinfIM [H]

Synonym: SPD_1260

Alternate gene names: 116516980

Gene position: 1288160-1289338 (Clockwise)

Preceding gene: 116516820

Following gene: 116516629

Centisome position: 62.96

GC content: 27.4

Gene sequence:

>1179_bases
ATGACAGTATTAAAAGGAGATAACTTAGAAATATTAAAAACTATTGAATCCTCAAGTATTGATTTAATCTATATGGACCC
TCCTTTCTTTACACAGAAAACCCAAAAATTATCTAATAACAAAAATATTATGTATTCATTCGAAGATACGTGGACTTCGA
TTGAGGATTACAAAGAATTTTTGTCTGTAAGATTAGAAGAATGCAAAAGAGTGCTAAAAAATAGTGGCAGTATTTTCGTT
CATTGTGATAAAATTGCAAATCATCATATTAGATTAATTTTAGATAATATCTTTGGAGCAGATATGTTTCAAAGCGAAAT
TATATGGAACTATAAACGATGGTCTAATTCAAAAAAGGGATTATTGAACAATCATCAAAACATTTACTTTTATTCAAAGT
CAAAAGATTTTAAATTTAATACAATTTTTACAGAGTATTCTTCTACTACAAATATCGACCAAATACTAGTGGAACGAAAA
CGAGATGGAAACTCTAAAACTATATATAAAGTTGATAATAATGGTAACTATATTCTAGCAAAAGAGAAAAATGGAGTTCC
CCTTTCAGATGTTTGGAATATACCATTTCTTAATCCAAAAGCTAAAGAAAGAGTAGGTTATCCTACACAAAAACCTATTC
TGTTATTAGAACAAATTATAAAGATTGCTACTGATAAAAATGATATAGTTTTAGACCCGTTCTGTGGAAGTGGAACTACT
TTAGTAGCCTCCAAGATTTTGAATAGAAATTATATGGGGATTGATTTATCTGAGGAAGCTATCAATATAACTCAGCAACG
TCTGGAAAATGTTATAAAAACAAGTTCAAATTTATTGAATAAAGGAATCGAAGCATATAGAACCAAAACTGAGGAAGAGG
AAAACATTCTTAAATTATTACAGGCAAAAATTGTTCAAAGAAATAAAGGAATTGATGGTTTTTTACCTAAACATTTTCAA
AAAAAACCGATACCTATAAAAATTCAAAAAAATAATGAATGTCTGAATGAGAGTATCTCTTTATTACAGAATGCTATAAA
CTCCAAAAAACTTGATTTTGGAGTAGTTATAAAAACTCATTCAGATAATTTATTATTTGATTTTGATACGATTCCTGAAA
ATATAATAGTAGTTGACCATTTTGAACTTACAATCGAAAAATGGTTAAGTAAAAGTTAA

Upstream 100 bases:

>100_bases
ATGGAGATTCTGCCTGGGAACATTTAAGAGCAGTGACCGGTATTGATTTACTTAGTATTCTAACAGATATTGCAAATAAA
AAAACAGGGGTAAAATCAAA

Downstream 100 bases:

>100_bases
CAGTTACTATAGTTTGCAAATTATTTACAGAAAGAACAAAAAATGCTCTGAAACACTGATTTCAAGGCATTTTTTGTTAG
TTTAAAATTACTCCCATTCT

Product: type II DNA modification methyltransferase

Products: NA

Alternate protein names: M.HinfI; Adenine-specific methyltransferase HinfI [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIMYSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFV
HCDKIANHHIRLILDNIFGADMFQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
RDGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQIIKIATDKNDIVLDPFCGSGTT
LVASKILNRNYMGIDLSEEAINITQQRLENVIKTSSNLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQ
KKPIPIKIQKNNECLNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS

Sequences:

>Translated_392_residues
MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIMYSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFV
HCDKIANHHIRLILDNIFGADMFQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
RDGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQIIKIATDKNDIVLDPFCGSGTT
LVASKILNRNYMGIDLSEEAINITQQRLENVIKTSSNLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQ
KKPIPIKIQKNNECLNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
>Mature_391_residues
TVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIMYSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVH
CDKIANHHIRLILDNIFGADMFQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERKR
DGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQIIKIATDKNDIVLDPFCGSGTTL
VASKILNRNYMGIDLSEEAINITQQRLENVIKTSSNLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQK
KPIPIKIQKNNECLNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS

Specific function: This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the HinfI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=287, Percent_Identity=31.0104529616725, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 45300; Mature: 45168

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIMYSFEDTWTSIEDYKEF
CCEECCCCHHEEEEECCCCEEEEEECCCCCHHHHHHHCCCCCEEEEECCHHCCHHHHHHH
LSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADMFQSEIIWNYKRWSNSKKG
HHHHHHHHHHHHHCCCCEEEEEHHHCCCEEEEEEEHHCCCHHHHHHHHCCHHHHCCCCCC
LLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERKRDGNSKTIYKVDNNGNYILA
CCCCCCEEEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCEEEE
KEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQIIKIATDKNDIVLDPFCGSGTT
ECCCCCCHHHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHH
LVASKILNRNYMGIDLSEEAINITQQRLENVIKTSSNLLNKGIEAYRTKTEEEENILKLL
HHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNECLNESISLLQNAINSKKLDFGVVIKTH
HHHHHHHCCCCCCCCCHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEEC
SDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
CCCEEEEECCCCCCEEEEEEHHHHHHHHHCCC
>Mature Secondary Structure 
TVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIMYSFEDTWTSIEDYKEF
CEECCCCHHEEEEECCCCEEEEEECCCCCHHHHHHHCCCCCEEEEECCHHCCHHHHHHH
LSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADMFQSEIIWNYKRWSNSKKG
HHHHHHHHHHHHHCCCCEEEEEHHHCCCEEEEEEEHHCCCHHHHHHHHCCHHHHCCCCCC
LLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERKRDGNSKTIYKVDNNGNYILA
CCCCCCEEEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCEEEE
KEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQIIKIATDKNDIVLDPFCGSGTT
ECCCCCCHHHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHH
LVASKILNRNYMGIDLSEEAINITQQRLENVIKTSSNLLNKGIEAYRTKTEEEENILKLL
HHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNECLNESISLLQNAINSKKLDFGVVIKTH
HHHHHHHCCCCCCCCCHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEEC
SDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
CCCEEEEECCCCCCEEEEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3063606 [H]