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The map label for this gene is trpE [H]

Identifier: 116516925

GI number: 116516925

Start: 1616971

End: 1618332

Strand: Reverse

Name: trpE [H]

Synonym: SPD_1602

Alternate gene names: 116516925

Gene position: 1618332-1616971 (Counterclockwise)

Preceding gene: 116515981

Following gene: 116516075

Centisome position: 79.09

GC content: 43.91

Gene sequence:

>1362_bases
ATGGAACGAATCATTCATGGAGATGTCTTATCACCAATCTTGGCTTATATGCGCTTAAAGGGGCAACACAAGGTTATCTT
AGAGAGTATTCCGAGAGACAAGGAAACAGCTCGTTTTTCTATCCTAGCCTATAATCCTGTTTTTGAGATTAAGTTTGAAA
ATGGAGTTCTTTATCAAAATGGTCAAGTGATTGATCGGGATCCCTTGGATTTCCTTTATGAAGTGATTCATAAGAGTCAG
CACCATTCAGAGCTACCTTTTGGTGGTGGAGCCATTGGTTTTGTGGGTTACGATATGATTTCGCTTTATGAAGAAATTGG
TCAAATCCCTGAGGATACAATTGGGACGCCAGACATGCATTTCTTTGTCTATGAGAGCTATATAGTCTTTGACCACAAGA
AGGAGAAGATACATGTCATAGAGGATGCATTTTATAGTGAGCGCAGTCAAGAGGCCTTGGAAAAATCCTTGAACCAAGTG
CTTGAGGAATTACGCATTCCTGCTCCAAATGAATTTGAAGACTTGGATTTATCACCACTTGACTTCAAACCGCATATCGC
TCCACATAAGTTTGAGGGAATGGTAGAAACAGCTCGTGACTTGATTCGTAACGGAGATATGTTCCAATGTGTACTCAGCC
AGCGTTTCTCAGCAGAAGTTACTGGAAATCCATTTGACTTCTACAGAAATCTCCGCGTAACTAATCCTTCTAATTACCTT
TATTTCTATGATTTTGGGGATTATCAAATCATCGGAGCTAGTCCAGAAAGTTTGGTTTCTGTCAAAAATGGCATCGTGAC
AACCAATCCGATTGCAGGGACGCGACCAAGAGGGGCTACGGATGAAGAAGACAAGGCCTTGGCGACTGACCTCCTCTCTG
ATGAGAAGGAAACAGCAGAACATCGAATGTTGGTAGACTTGGGGCGTAACGATATTGGCCGCATCTCTGAAACGACCAGT
GTCCAAGTCACTAAGTATATGGAAGTGGAGCTCTTCCGCTACGTCATGCATTTGACCAGCGTAGTCAAGGGGCGTTTGCT
TGCAGAACTCACTGCCATGGATGCCTTGAAAGCAACGCTTCCTGCTGGAACCGTTTCTGGAGCACCAAAGATTCGAGCGA
TGAGACGTATCTATGAACTGGAAACGGAAAAACGGGGAGTATACGCTGGGGCAATCGGCTACTTGTCTGCGACGGGTGAT
ATGGATTTGGCCATCGCCATCCGAACCATGATTCTCAAAAATCAAAGAGCCTATGTGCAGGCTGGGGCAGGGATTGTCTA
CGACTCTATCGCCCAAAACGAATACCAAGAAACCATTAACAAAGCAAAATCTATGACTAGAATTGGAGAACTAAGACCAT
GA

Upstream 100 bases:

>100_bases
GAGGTGGCACCGCGAATTTCGTCCTCACGCAAGTTATTTTGCGTGGGGATTTTTCATACAGTCGCTACGAACTAGAATTA
GAACTGAAAGGGAAATTACA

Downstream 100 bases:

>100_bases
TTTTATTGATTGACAACTATGATTCTTTTACCTATAACTTGGCCCAATACATTGGGAATTTTGCAGAAGTTCAGGTTCTG
AGAAATGATGATTCCAAGCT

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAYNPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQ
HHSELPFGGGAIGFVGYDMISLYEEIGQIPEDTIGTPDMHFFVYESYIVFDHKKEKIHVIEDAFYSERSQEALEKSLNQV
LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSAEVTGNPFDFYRNLRVTNPSNYL
YFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRPRGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETTS
VQVTKYMEVELFRYVMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAIGYLSATGD
MDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGELRP

Sequences:

>Translated_453_residues
MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAYNPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQ
HHSELPFGGGAIGFVGYDMISLYEEIGQIPEDTIGTPDMHFFVYESYIVFDHKKEKIHVIEDAFYSERSQEALEKSLNQV
LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSAEVTGNPFDFYRNLRVTNPSNYL
YFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRPRGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETTS
VQVTKYMEVELFRYVMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAIGYLSATGD
MDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGELRP
>Mature_453_residues
MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAYNPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQ
HHSELPFGGGAIGFVGYDMISLYEEIGQIPEDTIGTPDMHFFVYESYIVFDHKKEKIHVIEDAFYSERSQEALEKSLNQV
LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSAEVTGNPFDFYRNLRVTNPSNYL
YFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRPRGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETTS
VQVTKYMEVELFRYVMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAIGYLSATGD
MDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGELRP

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=396, Percent_Identity=36.6161616161616, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1788114, Length=431, Percent_Identity=32.4825986078886, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1786809, Length=262, Percent_Identity=26.7175572519084, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082077, Length=264, Percent_Identity=26.8939393939394, Blast_Score=75, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320935, Length=454, Percent_Identity=39.4273127753304, Blast_Score=286, Evalue=4e-78,
Organism=Saccharomyces cerevisiae, GI6324361, Length=407, Percent_Identity=27.5184275184275, Blast_Score=124, Evalue=3e-29,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 51202; Mature: 51202

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAYNPVFEIKFENGVLYQN
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEEEECCEEEEEECCCEEEEC
GQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISLYEEIGQIPEDTIGTPDMH
CEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
FFVYESYIVFDHKKEKIHVIEDAFYSERSQEALEKSLNQVLEELRIPAPNEFEDLDLSPL
EEEEEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSAEVTGNPFDFYRNLRVTNPSNYL
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHCCCEEECCCCEE
YFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRPRGATDEEDKALATDLLSDEKETAE
EEEECCCEEEECCCHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHH
HRMLVDLGRNDIGRISETTSVQVTKYMEVELFRYVMHLTSVVKGRLLAELTAMDALKATL
HHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PAGTVSGAPKIRAMRRIYELETEKRGVYAGAIGYLSATGDMDLAIAIRTMILKNQRAYVQ
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEEHHEEEECCCCCHHHHHHHHHHHHCCHHEEE
AGAGIVYDSIAQNEYQETINKAKSMTRIGELRP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAYNPVFEIKFENGVLYQN
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEEEECCEEEEEECCCEEEEC
GQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISLYEEIGQIPEDTIGTPDMH
CEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
FFVYESYIVFDHKKEKIHVIEDAFYSERSQEALEKSLNQVLEELRIPAPNEFEDLDLSPL
EEEEEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSAEVTGNPFDFYRNLRVTNPSNYL
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHCCCEEECCCCEE
YFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRPRGATDEEDKALATDLLSDEKETAE
EEEECCCEEEECCCHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHH
HRMLVDLGRNDIGRISETTSVQVTKYMEVELFRYVMHLTSVVKGRLLAELTAMDALKATL
HHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PAGTVSGAPKIRAMRRIYELETEKRGVYAGAIGYLSATGDMDLAIAIRTMILKNQRAYVQ
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEEHHEEEECCCCCHHHHHHHHHHHHCCHHEEE
AGAGIVYDSIAQNEYQETINKAKSMTRIGELRP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400208; 11337471 [H]