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The map label for this gene is 116516894

Identifier: 116516894

GI number: 116516894

Start: 832940

End: 833815

Strand: Direct

Name: 116516894

Synonym: SPD_0815

Alternate gene names: NA

Gene position: 832940-833815 (Clockwise)

Preceding gene: 116516045

Following gene: 116516715

Centisome position: 40.71

GC content: 43.04

Gene sequence:

>876_bases
ATGAGAAATGTAAGAGTTGCAACCATTCAGATGCAATGCGCTAAGGATGTGGCAACAAATATCCAAACCGCAGAGCGTTT
AGTACGTCAGGCTGCTGAGCAAGGAGCCCAAATTATTCTCTTGCCCGAGTTGTTTGAACATCCCTATTTCTGTCAGGAAC
GTCAGTATGACTACTACCAGTATGCCCAATCTGTAGCGGAAAATACTGCCATTCAGCATTTTAAGGTGATTGCTAAGGAA
CTACAAGTTGTTTTACCAATCAGTTTCTATGAAAAAGATGGTAATGTCTTGTATAACTCTATTGCCGTCATTGATGCAGA
TGGGGAAGTGCTGGGCGTTTATCGAAAGACCCATATACCAGATGACCATTATTATCAAGAAAAATTCTATTTCACGCCTG
GTAACACTGGTTTCAAGGTCTGGAATACTCGCTATGCTAAGATTGGTATCGGTATCTGTTGGGATCAATGGTTCCCTGAA
ACAGCGCGCTGTCTTGCATTGAATGGTGCTGAATTGCTCTTTTATCCTACAGCTATCGGTTCAGAGCCAATTTTGGATAC
AGATAGTTGTGGTCACTGGCAACGTACTATGCAAGGGCACGCAGCAGCGAATATTGTTCCAGTCATCGCAGCCAATCGTT
ATGGTTTAGAGGAGGTTACTCCTAGTGAGGAAAATGGCGGACAGAGTTCCAGTCTTGACTTCTACGGTTCCTCCTTTATG
ACGGATGAAACAGGAGCTATTCTAGAACGAGCTGAAAGACAAGAAGAAGCTGTTCTGTTAGCTACTTATAACCTAGACAA
GGGAGCAAGTGAACGCCTAAACTGGGGCTTGTTTCGAGATAGAAGACCAGAAATGTATAGACAAATTACAGATTAG

Upstream 100 bases:

>100_bases
CCCAGACCGTAAAGTTGTCGGAATACCAGCCAGAGATATTCTCTTAGGTGGTGGCAATATCCACTGTATCACCCAACAAA
TTCCAGAATAGGAGAAAAAG

Downstream 100 bases:

>100_bases
TGTGGGAGAAATGAGAGATTCATTCTGCTAGACTAACTTCTTATTAGCAACTATAAGATACTATGGCATCTAGTAAATCG
ATTTTTATGATTCGCTATTC

Product: hydrolase, carbon-nitrogen family protein

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPELFEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKE
LQVVLPISFYEKDGNVLYNSIAVIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEEVTPSEENGGQSSSLDFYGSSFM
TDETGAILERAERQEEAVLLATYNLDKGASERLNWGLFRDRRPEMYRQITD

Sequences:

>Translated_291_residues
MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPELFEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKE
LQVVLPISFYEKDGNVLYNSIAVIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEEVTPSEENGGQSSSLDFYGSSFM
TDETGAILERAERQEEAVLLATYNLDKGASERLNWGLFRDRRPEMYRQITD
>Mature_291_residues
MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPELFEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKE
LQVVLPISFYEKDGNVLYNSIAVIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEEVTPSEENGGQSSSLDFYGSSFM
TDETGAILERAERQEEAVLLATYNLDKGASERLNWGLFRDRRPEMYRQITD

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=292, Percent_Identity=31.1643835616438, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI7706509, Length=282, Percent_Identity=28.0141843971631, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI5031947, Length=292, Percent_Identity=27.0547945205479, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI297632348, Length=292, Percent_Identity=27.0547945205479, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI297632350, Length=292, Percent_Identity=27.0547945205479, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI297632346, Length=187, Percent_Identity=29.9465240641711, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17533173, Length=307, Percent_Identity=31.2703583061889, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17556280, Length=290, Percent_Identity=23.448275862069, Blast_Score=81, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323383, Length=299, Percent_Identity=25.0836120401338, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6322335, Length=309, Percent_Identity=23.6245954692557, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21358471, Length=304, Percent_Identity=27.6315789473684, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21355835, Length=291, Percent_Identity=24.7422680412371, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17933642, Length=288, Percent_Identity=25.3472222222222, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 33154; Mature: 33154

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPELFEHPYFCQERQYDYYQ
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCHHHH
YAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIAVIDADGEVLGVYRKTHIP
HHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEEEEEECCCCEEEEEECCCCC
DDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPETARCLALNGAELLFYPTAIG
CCCEECEEEEECCCCCCEEEECEEEEEEEEEEEECCCCCCCCEEEEECCCEEEEEECCCC
SEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEEVTPSEENGGQSSSLDFYGSSFM
CCCCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCC
TDETGAILERAERQEEAVLLATYNLDKGASERLNWGLFRDRRPEMYRQITD
CCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPELFEHPYFCQERQYDYYQ
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCHHHH
YAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIAVIDADGEVLGVYRKTHIP
HHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEEEEEECCCCEEEEEECCCCC
DDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPETARCLALNGAELLFYPTAIG
CCCEECEEEEECCCCCCEEEECEEEEEEEEEEEECCCCCCCCEEEEECCCEEEEEECCCC
SEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEEVTPSEENGGQSSSLDFYGSSFM
CCCCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCC
TDETGAILERAERQEEAVLLATYNLDKGASERLNWGLFRDRRPEMYRQITD
CCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]