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The map label for this gene is rlmN

Identifier: 116516867

GI number: 116516867

Start: 688885

End: 689970

Strand: Direct

Name: rlmN

Synonym: SPD_0669

Alternate gene names: 116516867

Gene position: 688885-689970 (Clockwise)

Preceding gene: 116516259

Following gene: 116515988

Centisome position: 33.67

GC content: 42.45

Gene sequence:

>1086_bases
ATGAAACCCTCAATTCATAGTTTGGCACATCAAACCATGCAGGAATGGGTATTGGAGCAGGGAGAAAAGAAATTCCGTGC
AGATCAAATCTGGGAATGGCTCTACCGTAAACGTGTGCAGTCATTTGAAGAAATGACCAACCTTTCCAAGGATTTGATTG
CTAAGCTCAATGACCAGTTTGTGGTCAATCCCTTGAAACAACGTATTGTGCAAGAGTCTGCTGATGGTACAGTCAAATAT
CTCTTTGAATTGCCTGACGGTATGTTAATTGAAACTGTACTCATGCGTCAACACTATGGTTTATCTGTCTGTGTGACCAC
TCAGGTCGACTGTAATATCGGTTGTACCTTCTGTGCCTCTGATTTGATTAAGAAGCAACGTGACCTCAATAACGGTGAAA
TCGTAGCGCAAATCATGCTGGTTCAGAAATACTTTGCTGAGCGTGGTCAGGATGAACGCGTCAGCCATATCGTTGTCATG
GGAATCGGTGAGCCCTTTGATAATTACAACAATGTCTTGAATTTCGTTTGTACTATCAATGATGATAAAGGAATGGCTAT
CGGTGCTCGTCACATCACGGTTTCAACCTCAGGTTTGGCTCATAAAATTCGTAACTTTGCTGATGAAGGGGTTCAGGTCA
ATCTTGCCGTGTCTCTTCACGCACCCAACAATGAACTTCGTTCAAGTATCATGAAGATTAACCGTGCCTTTCCGATTGAA
AAACTCTTTGCTGCTATTGAATACTACATTGAGACAACAAACCGTCGTGTAACCTTTGAATATATCATGCTTAATGAAGT
CAACGACAGTGTAGAACAAGCCTTGGAATTGGCTGAATTGCTCAAGAACATCAAGAAATTGTCTTATGTAAACTTGATTC
CTTATAACCCAGTTAGTGAGCATGACCAATATAGCCGTAGTCCCAAAGAGCGCGTGTTGGCTTTCTATGATACGCTTAAG
AAAAAAGGGGGCAACTGTGTGGTTCGTCAAGAGTATGGTACAGATATTGACGCAGCTTGTGGACAATTGCGCTCTAACAC
AATGAAACGTGACCGCCAGAAGGCAGTCGCAGAGGTAAATCCGTAA

Upstream 100 bases:

>100_bases
AAAATAGACGTCGTCAGAAAGACCAGCATTCTCAGAAAGAGGACAAGGAACAACGTCATTTTGTCATTCGTCAGAAGTGA
GTAGAGAATAAGGAGAAGAA

Downstream 100 bases:

>100_bases
AATGACTAAAAAAAGAGAATTAATCTTAAGATTGGGAGTGGCTGTTTATAGCCTATGCATTGTCTATTTTTGCTTTACTC
CTCAAGAACAATCGGGAACT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEEMTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKY
LFELPDGMLIETVLMRQHYGLSVCVTTQVDCNIGCTFCASDLIKKQRDLNNGEIVAQIMLVQKYFAERGQDERVSHIVVM
GIGEPFDNYNNVLNFVCTINDDKGMAIGARHITVSTSGLAHKIRNFADEGVQVNLAVSLHAPNNELRSSIMKINRAFPIE
KLFAAIEYYIETTNRRVTFEYIMLNEVNDSVEQALELAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLK
KKGGNCVVRQEYGTDIDAACGQLRSNTMKRDRQKAVAEVNP

Sequences:

>Translated_361_residues
MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEEMTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKY
LFELPDGMLIETVLMRQHYGLSVCVTTQVDCNIGCTFCASDLIKKQRDLNNGEIVAQIMLVQKYFAERGQDERVSHIVVM
GIGEPFDNYNNVLNFVCTINDDKGMAIGARHITVSTSGLAHKIRNFADEGVQVNLAVSLHAPNNELRSSIMKINRAFPIE
KLFAAIEYYIETTNRRVTFEYIMLNEVNDSVEQALELAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLK
KKGGNCVVRQEYGTDIDAACGQLRSNTMKRDRQKAVAEVNP
>Mature_361_residues
MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEEMTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKY
LFELPDGMLIETVLMRQHYGLSVCVTTQVDCNIGCTFCASDLIKKQRDLNNGEIVAQIMLVQKYFAERGQDERVSHIVVM
GIGEPFDNYNNVLNFVCTINDDKGMAIGARHITVSTSGLAHKIRNFADEGVQVNLAVSLHAPNNELRSSIMKINRAFPIE
KLFAAIEYYIETTNRRVTFEYIMLNEVNDSVEQALELAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLK
KKGGNCVVRQEYGTDIDAACGQLRSNTMKRDRQKAVAEVNP

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=357, Percent_Identity=37.5350140056022, Blast_Score=231, Evalue=5e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_STRP2 (Q04LD5)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816163.1
- ProteinModelPortal:   Q04LD5
- STRING:   Q04LD5
- EnsemblBacteria:   EBSTRT00000020614
- GeneID:   4442149
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0669
- eggNOG:   COG0820
- GeneTree:   EBGT00050000027236
- HOGENOM:   HBG335345
- OMA:   VQKYFDE
- ProtClustDB:   PRK14455
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41307; Mature: 41307

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEEMTNLSKDLIAKLNDQF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLSVCVTTQVDCNIGCTFCAS
EECHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHH
DLIKKQRDLNNGEIVAQIMLVQKYFAERGQDERVSHIVVMGIGEPFDNYNNVLNFVCTIN
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHEEEEEEEE
DDKGMAIGARHITVSTSGLAHKIRNFADEGVQVNLAVSLHAPNNELRSSIMKINRAFPIE
CCCCCEEEEEEEEEECHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCHH
KLFAAIEYYIETTNRRVTFEYIMLNEVNDSVEQALELAELLKNIKKLSYVNLIPYNPVSE
HHHHHHHHHHHHCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
HDQYSRSPKERVLAFYDTLKKKGGNCVVRQEYGTDIDAACGQLRSNTMKRDRQKAVAEVN
CHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
P
C
>Mature Secondary Structure
MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEEMTNLSKDLIAKLNDQF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLSVCVTTQVDCNIGCTFCAS
EECHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHH
DLIKKQRDLNNGEIVAQIMLVQKYFAERGQDERVSHIVVMGIGEPFDNYNNVLNFVCTIN
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHEEEEEEEE
DDKGMAIGARHITVSTSGLAHKIRNFADEGVQVNLAVSLHAPNNELRSSIMKINRAFPIE
CCCCCEEEEEEEEEECHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCHH
KLFAAIEYYIETTNRRVTFEYIMLNEVNDSVEQALELAELLKNIKKLSYVNLIPYNPVSE
HHHHHHHHHHHHCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
HDQYSRSPKERVLAFYDTLKKKGGNCVVRQEYGTDIDAACGQLRSNTMKRDRQKAVAEVN
CHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA