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The map label for this gene is ddl

Identifier: 116516832

GI number: 116516832

Start: 1499921

End: 1500964

Strand: Reverse

Name: ddl

Synonym: SPD_1484

Alternate gene names: 116516832

Gene position: 1500964-1499921 (Counterclockwise)

Preceding gene: 116516007

Following gene: 116515757

Centisome position: 73.36

GC content: 44.92

Gene sequence:

>1044_bases
ATGAAACAAACGATTATTCTTTTATATGGTGGACGGAGTGCGGAACGCGAAGTCTCTGTCCTTTCAGCTGAGAGTGTCAT
GCGTGCGGTCGATTACGACCGTTTCACAGTCAAGACTTTCTTTATCAGTCAGTCAGGTGACTTTATCAAAACACAGGAAT
TTAGTCATGCTCCGGGGCAAGAAGACCGTCTCATGACCAATGAAACCATTGATTGGGATAAGAAAGTTGCACCAAGTGCT
ATCTACGAAGAAGGTGCAGTGGTCTTTCCAGTCCTTCACGGGCCAATGGGAGAAGATGGCTCTGTTCAAGGATTCTTGGA
AGTTTTGAAAATGCCTTACGTTGGTTGCAACATTTTGTCATCAAGTCTTGCCATGGATAAAATCACGACTAAGCGTGTTC
TGGAATCTGCTGGTATTGCCCAAGTTCCTTATGTGGCTATCGTTGAAGGCGATGATGTGACTGCTAAAATCGCTGAAGTG
GAAGAAAAATTGGCTTATCCAGTCTTCACTAAGCCGTCAAACATGGGGTCTAGTGTCGGTATTTCTAAGTCTGAAAACCA
AGAAGAACTCCGTCAAGCCTTAAAACTTGCCTTCCGATATGACAGCCGTGTCTTGGTTGAGCAAGGAGTGAATGCCCGTG
AAATTGAGGTTGGCCTCTTGGGTAACTACGATGTCAAGAGCACGCTACCTGGAGAAGTTGTCAAGGACGTTGCCTTTTAT
GACTACGATGCCAAGTATATTGATAACAAGGTTACTATGGATATTCCTGCCAAAATCAGTGATGATGTGGTGGCTGTCAT
GCGTCAAAATGCAGAAACAGCCTTCCGTGCCATTGGTGGCCTTGGTCTATCTCGTTGCGATTTCTTCTATACAGATAAGG
GAGAGATTTTTCTCAACGAGCTCAATACCATGCCAGGTTTCACCCAGTGGTCTATGTACCCACTACTTTGGGAAAATATG
GGGATCAGCTACCCAGAACTAATCGAGCGTTTGGTTGACCTTGCCAAGGAAAGTTTTGACAAGCGCGAAGCGCATTTGAT
ATAA

Upstream 100 bases:

>100_bases
TTGTATAGTGTGATGAGTAGGCTCAGGTTCAAGTTTTAAAAAACCAAGCAAATATGATATACTAAAGAGCGAGTATTCTA
GTAGAATTAGGACAAATAAT

Downstream 100 bases:

>100_bases
AATGAAAGAGAGGGTAGAAGCCAGAACCATCACTGCAAGGTGACTAGAGTTCTCGGACTTCAGCCCTTTTTAAAGGAGTA
GAAATGAAATTAACAATCCA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQSGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSA
IYEEGAVVFPVLHGPMGEDGSVQGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVGLLGNYDVKSTLPGEVVKDVAFY
DYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFRAIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENM
GISYPELIERLVDLAKESFDKREAHLI

Sequences:

>Translated_347_residues
MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQSGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSA
IYEEGAVVFPVLHGPMGEDGSVQGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVGLLGNYDVKSTLPGEVVKDVAFY
DYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFRAIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENM
GISYPELIERLVDLAKESFDKREAHLI
>Mature_347_residues
MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQSGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSA
IYEEGAVVFPVLHGPMGEDGSVQGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVGLLGNYDVKSTLPGEVVKDVAFY
DYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFRAIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENM
GISYPELIERLVDLAKESFDKREAHLI

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=353, Percent_Identity=37.3937677053824, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1786280, Length=344, Percent_Identity=31.9767441860465, Blast_Score=163, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_STRP2 (Q04J98)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816940.1
- ProteinModelPortal:   Q04J98
- SMR:   Q04J98
- STRING:   Q04J98
- EnsemblBacteria:   EBSTRT00000019655
- GeneID:   4442150
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1484
- eggNOG:   COG1181
- GeneTree:   EBGT00050000027793
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 38719; Mature: 38719

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQSGDFIKTQEFSHAPGQ
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEECCCCCCCCC
EDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSVQGFLEVLKMPYVGCNILS
CCCEECCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHH
SSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEVEEKLAYPVFTKPSNMGSSVG
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEECCCCCCCCCC
ISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVGLLGNYDVKSTLPGEVVKDVAFY
CCCCCCHHHHHHHHHHHHHCCCEEHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHC
DYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFRAIGGLGLSRCDFFYTDKGEIFLNE
CCCHHHCCCEEEEEECCCCCHHHHHHHHHCHHHHHHHHHCCCHHHCEEEECCCCCEEEEE
LNTMPGFTQWSMYPLLWENMGISYPELIERLVDLAKESFDKREAHLI
CCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHCCCC
>Mature Secondary Structure
MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQSGDFIKTQEFSHAPGQ
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEECCCCCCCCC
EDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSVQGFLEVLKMPYVGCNILS
CCCEECCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHH
SSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEVEEKLAYPVFTKPSNMGSSVG
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEECCCCCCCCCC
ISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVGLLGNYDVKSTLPGEVVKDVAFY
CCCCCCHHHHHHHHHHHHHCCCEEHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHC
DYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFRAIGGLGLSRCDFFYTDKGEIFLNE
CCCHHHCCCEEEEEECCCCCHHHHHHHHHCHHHHHHHHHCCCHHHCEEEECCCCCEEEEE
LNTMPGFTQWSMYPLLWENMGISYPELIERLVDLAKESFDKREAHLI
CCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA