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The map label for this gene is nplT [H]

Identifier: 116516706

GI number: 116516706

Start: 939476

End: 941239

Strand: Direct

Name: nplT [H]

Synonym: SPD_0927

Alternate gene names: 116516706

Gene position: 939476-941239 (Clockwise)

Preceding gene: 116517124

Following gene: 116515723

Centisome position: 45.92

GC content: 39.23

Gene sequence:

>1764_bases
ATGAATACTTTAGGAGACAAATTGATGGAATTGAGTGCTATTTACCATGGGCCTGAGTCGGAGTATGCCTATCTTTATAA
GGATAAGAAACTCCATATTCGAATTCGAACTAAGAAAGGGGACATTGAAAGCATCAACTTGCACTATGGGGACCCTTTTA
TCTTTATGGAGGAGTTTTATCAGGATACAAAAGAAATGGTCAAGATAACTTCTGGTACCTTATTTGACCATTGGCAGGTT
GAAGTGTCAGTTGACTTTGCACGTATCCAGTATCTCTTTGAGCTCAGAGATACAGAAGGTCAAAATATTTTGTATGGCGA
TAAAGGGTGTGTGGAAAATTCTCTAGAAAATCTTCATGCAATTGGGAATGGATTTAAGTTGCCTTATCTTCATGAGATTG
ATGCCTGCAAGGTTCCTGACTGGGTTTCAAATACGGTATGGTATCAGATATTTCCTGAAAGATTTGCCAATGGCAATGCT
CTATTAAACCCAGAAGGGACTTTAGACTGGGATTCATCTGTCACACCTAAGAGCGATGATTTCTTTGGTGGTGATTTACA
GGGGATTATTGATCATATGGATTACTTGCAAGACTTGGGTATTACTGGACTATATCTTTGTCCCATCTTTGAATCTACAA
GCAATCACAAGTACAATACGACAGATTACTTTGAAATTGACCGTCATTTTGGAGACAAGGAGACCTTTCGGGAACTGGTG
GATCAAGCGCATCATCGTGGCATGAAAGTCATGCTGGATGCGGTATTTAATCATATTGCTTCGCAATCTCTTCAATGGAA
AAATGTCGTCAAAAATGGTGAACAGTCTGCTTATAAGGATTGGTTCCATATTCAACAATTCCCAGTGACAACTGAAAAGC
TAGTTAATAAGAGAGACTTACCCTATCATGTTTTTGGTTTCGAGGACTATATGCCTAAGCTAAATACAGCCAATCCAGAG
GTCAAGAATTATCTTTTAAAGGTTGCGACTTATTGGATTGAAGAGTTTAATATCGATGCTTGGCGTTTGGATGTGGCTAA
TGAGATTGACCATCAGTTCTGGAAGGATTTTCGTAAGGCAGTTTTAGCTAAAAATCCTGATCTTTATATCCTAGGAGAAG
TCTGGCATACATCTCAGCCTTGGCTAAATGGAGATGAGTTCCATGCCGTCATGAATTATCCTTTATCTGATAGTATCAAG
GACTATTTCTTACGAGGAATTAAGAAGACAGACCAGTTCATCGATGAAATCAATGGAGAGTCTATGTATTACAAGCAGCA
GATTTCAGAGGTCATGTTTAATCTCTTGGATTCACATGATACAGAGCGAATCCTGTGGACGGCCAATGAAGATGTTCAAC
TGGTTAAATCAGCCTTAGCCTTTCTCTTTTTACAAAAAGGAACACCGTGCATTTATTACGTAACCGAGCTAGCCTTGACT
GGAGGACCAGATCCAGATTGTCGTCGTTGTATGCCTTGGGAACGTGTATCAAGTGACAATGATATGCTGAACTTTATGAA
GAGGCTGATTAAGATTCGGAAATACGCGTCAGTAATCATTTCGCATGGCAAGTATAGCCTTCAAGAAATCAACTCTGATC
TAGTAGCTCTGGAATGGAAATACGAAGGACGGATCCTCAAAGCAATATTCAACCAATCAACAGAAGATTATCTTTTAGAG
AAAGAAGCAGTAGCACTAGCAAGCAATTGCCAAGAATTGGATAATCAGCTTGTCATCTCTCCAGATGGATTTATGATTTT
CTAA

Upstream 100 bases:

>100_bases
TTAAAAGATAGTTCCTTTAACAACGGACATTCCTTGCAAATAGTTTACAAAAATAGTATACTGGATTTATTGAGTTTGAA
AACGTTTGCGTAAAATTTGA

Downstream 100 bases:

>100_bases
AAACTAGTTGATGAAGATTATGGTACATTTCATACCTTATATAGTATAATAAGGCTAGTTACTAAACTTGTAAAGGAGAA
CTTAAATGAATTGTAGAGGA

Product: neopullulanase

Products: alpha-D-glucose; beta-D-glucose; amylose(n-1) [C]

Alternate protein names: NA

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MNTLGDKLMELSAIYHGPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDPFIFMEEFYQDTKEMVKITSGTLFDHWQV
EVSVDFARIQYLFELRDTEGQNILYGDKGCVENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNA
LLNPEGTLDWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKETFRELV
DQAHHRGMKVMLDAVFNHIASQSLQWKNVVKNGEQSAYKDWFHIQQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPE
VKNYLLKVATYWIEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSALAFLFLQKGTPCIYYVTELALT
GGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYASVIISHGKYSLQEINSDLVALEWKYEGRILKAIFNQSTEDYLLE
KEAVALASNCQELDNQLVISPDGFMIF

Sequences:

>Translated_587_residues
MNTLGDKLMELSAIYHGPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDPFIFMEEFYQDTKEMVKITSGTLFDHWQV
EVSVDFARIQYLFELRDTEGQNILYGDKGCVENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNA
LLNPEGTLDWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKETFRELV
DQAHHRGMKVMLDAVFNHIASQSLQWKNVVKNGEQSAYKDWFHIQQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPE
VKNYLLKVATYWIEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSALAFLFLQKGTPCIYYVTELALT
GGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYASVIISHGKYSLQEINSDLVALEWKYEGRILKAIFNQSTEDYLLE
KEAVALASNCQELDNQLVISPDGFMIF
>Mature_587_residues
MNTLGDKLMELSAIYHGPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDPFIFMEEFYQDTKEMVKITSGTLFDHWQV
EVSVDFARIQYLFELRDTEGQNILYGDKGCVENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNA
LLNPEGTLDWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKETFRELV
DQAHHRGMKVMLDAVFNHIASQSLQWKNVVKNGEQSAYKDWFHIQQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPE
VKNYLLKVATYWIEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSALAFLFLQKGTPCIYYVTELALT
GGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYASVIISHGKYSLQEINSDLVALEWKYEGRILKAIFNQSTEDYLLE
KEAVALASNCQELDNQLVISPDGFMIF

Specific function: Hydrolyzes beta-cyclodextrin to maltose and glucose, soluble starch to maltose and glucose, and pullulan to panose with trace amounts of maltose and glucose. It is also able to hydrolyze acarbose. Can also exhibit a transglycosylation activity transferrin

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. BbmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=233, Percent_Identity=24.0343347639485, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1786604, Length=488, Percent_Identity=28.6885245901639, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1790687, Length=414, Percent_Identity=26.3285024154589, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1789995, Length=160, Percent_Identity=30.625, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321726, Length=287, Percent_Identity=22.9965156794425, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322245, Length=229, Percent_Identity=23.5807860262009, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321731, Length=145, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319776, Length=145, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324416, Length=289, Percent_Identity=22.4913494809689, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322241, Length=289, Percent_Identity=22.4913494809689, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322021, Length=289, Percent_Identity=22.4913494809689, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24586587, Length=230, Percent_Identity=27.3913043478261, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI221330053, Length=243, Percent_Identity=27.9835390946502, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24586599, Length=241, Percent_Identity=27.8008298755187, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583747, Length=229, Percent_Identity=28.82096069869, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583749, Length=229, Percent_Identity=28.82096069869, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24586597, Length=225, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24586589, Length=263, Percent_Identity=26.9961977186312, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583745, Length=225, Percent_Identity=28.4444444444444, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24586591, Length=225, Percent_Identity=25.7777777777778, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45549022, Length=226, Percent_Identity=25.6637168141593, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24586593, Length=223, Percent_Identity=26.0089686098655, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: 3.2.1.20 [C]

Molecular weight: Translated: 68492; Mature: 68492

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLGDKLMELSAIYHGPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDPFIFMEEFY
CCCHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEEECCCCCEEEEEECCCCHHHHHHHH
QDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGCVENSLENLHA
HHHHHHHHHHCCCEEEEEEEEEEEHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHH
IGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTLDWDSSVTPKSDD
HCCCCCCCCEECCCCCCCCCHHCCCEEEEECHHHHCCCCEEECCCCCCCCCCCCCCCCCC
FFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKETFRELV
CCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEHHHCCCHHHHHHHH
DQAHHRGMKVMLDAVFNHIASQSLQWKNVVKNGEQSAYKDWFHIQQFPVTTEKLVNKRDL
HHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHCCCCCC
PYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWRLDVANEIDHQFWKDFRKA
CEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHH
VLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIKDYFLRGIKKTDQFIDEINGE
HHCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
SMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSALAFLFLQKGTPCIYYVTELALT
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHCC
GGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYASVIISHGKYSLQEINSDLVALEWK
CCCCCCHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEE
YEGRILKAIFNQSTEDYLLEKEAVALASNCQELDNQLVISPDGFMIF
ECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEC
>Mature Secondary Structure
MNTLGDKLMELSAIYHGPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDPFIFMEEFY
CCCHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEEECCCCCEEEEEECCCCHHHHHHHH
QDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGCVENSLENLHA
HHHHHHHHHHCCCEEEEEEEEEEEHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHH
IGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTLDWDSSVTPKSDD
HCCCCCCCCEECCCCCCCCCHHCCCEEEEECHHHHCCCCEEECCCCCCCCCCCCCCCCCC
FFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKETFRELV
CCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEHHHCCCHHHHHHHH
DQAHHRGMKVMLDAVFNHIASQSLQWKNVVKNGEQSAYKDWFHIQQFPVTTEKLVNKRDL
HHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHCCCCCC
PYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWRLDVANEIDHQFWKDFRKA
CEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHH
VLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIKDYFLRGIKKTDQFIDEINGE
HHCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
SMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSALAFLFLQKGTPCIYYVTELALT
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHCC
GGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYASVIISHGKYSLQEINSDLVALEWK
CCCCCCHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEE
YEGRILKAIFNQSTEDYLLEKEAVALASNCQELDNQLVISPDGFMIF
ECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): {} [C]

Substrates: Maltose; H2O; 1,4-alpha-D-glucan [C]

Specific reaction: Maltose + H2O = 2 alpha-D-glucose H2O + 1,4-alpha-D-glucan = beta-D-glucose + amylose(n-1) [C]

General reaction: O-Glycosyl bond hydrolysis; Exohydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]