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The map label for this gene is rnz

Identifier: 116516699

GI number: 116516699

Start: 612662

End: 613591

Strand: Direct

Name: rnz

Synonym: SPD_0586

Alternate gene names: 116516699

Gene position: 612662-613591 (Clockwise)

Preceding gene: 116516854

Following gene: 116515980

Centisome position: 29.94

GC content: 45.27

Gene sequence:

>930_bases
ATGGATATTCAATTTTTAGGAACGGGGGCTGGTCAGCCCTCTAAAGCCCGCAACGTTTCAAGTCTCGCCCTGAAACTTTT
GGATGAGATTAACGAAGTTTGGCTCTTTGACTGTGGAGAAGGTACGCAAAATCGCATTCTGGAAACCACAATTCGACCAC
GTAAGGTCAGCAAAATCTTTATTACCCATCTGCATGGAGACCACATTTTTGGTTTGCCAGGTTTCCTTTCTAGCCGTGCC
TTTCAGGCCAATGAAGAGCAGACAGATTTGGAAATCTACGGACCTCAAGGAATCAAGTCATTTGTCTTAACCAGCCTTCG
TGTGTCAGGTTCTCGTCTGCCCTACCGCATTCATTTCCATGAGTTTGACCAAGATTCTCTGGGTAAAATTCTTGAAACCG
ATAAATTCACTGTGTATGCAGAGGAGCTGGACCACACTATTTTCTGTGTTGGCTATCGTGTCATGCAAAAGGATCTAGAA
GGGACGCTGGATGCTGAAAAACTCAAGGCTGCTGGTGTTCCGTTCGGCCCGCTTTTTGGTAAAATCAAAAACGGCCAGGA
TCTTGTTTTGGAAGACGGAACTGAAATCAAGGCAGCAGACTATATCTCAGCGCCACGTCCAGGTAAGATTATCACTATTT
TAGGAGACACTCGAAAAACGGGTGCCAGTGTGCGTCTGGCTGTCAATGCAGATGTCCTAGTTCATGAGTCCACTTATGGC
AAGGGTGATGAAAAAATTGCTCGTAACCATGGTCACTCAACTAATATGCAAGCTGCACAAGTAGCGGTAGAAGCAGGTGC
CAAACGCCTCCTACTCAACCATATCAGTGCCCGTTTCCTCTCAAAAGATATTAGCAAACTCAAGAAAGACGCTGCCACAA
TTTTTGAAAATGTCCATGTGGTCAAAGACTTGGAAGAAGTGGAAATCTAG

Upstream 100 bases:

>100_bases
AGGATTTATTGCAAGAAGCTGCTGCTTTTTCTGGCAAACGCTATTTCCGATCGGAAAACCGCACAGCCATGTTATATATT
GATTAATTAGAAAGTATCGA

Downstream 100 bases:

>100_bases
CAGTCACAGAAAGGATAAGTATGCCTACTATTCTCATTACCGGAGCTAGCGGTGGTCTAGCTCAAGAAATGGTCAAACTC
CTGCCCAATGACCAACTCAT

Product: ribonuclease Z

Products: NA

Alternate protein names: RNase Z; tRNA 3 endonuclease; tRNase Z

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQNRILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRA
FQANEEQTDLEIYGPQGIKSFVLTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTRKTGASVRLAVNADVLVHESTYG
KGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHISARFLSKDISKLKKDAATIFENVHVVKDLEEVEI

Sequences:

>Translated_309_residues
MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQNRILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRA
FQANEEQTDLEIYGPQGIKSFVLTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTRKTGASVRLAVNADVLVHESTYG
KGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHISARFLSKDISKLKKDAATIFENVHVVKDLEEVEI
>Mature_309_residues
MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQNRILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRA
FQANEEQTDLEIYGPQGIKSFVLTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTRKTGASVRLAVNADVLVHESTYG
KGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHISARFLSKDISKLKKDAATIFENVHVVKDLEEVEI

Specific function: Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA

COG id: COG1234

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase Z family

Homologues:

Organism=Homo sapiens, GI8922122, Length=358, Percent_Identity=36.5921787709497, Blast_Score=213, Evalue=2e-55,
Organism=Escherichia coli, GI87082078, Length=305, Percent_Identity=45.2459016393443, Blast_Score=267, Evalue=8e-73,
Organism=Drosophila melanogaster, GI24652403, Length=212, Percent_Identity=30.6603773584906, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNZ_STRP2 (Q04LL3)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816085.1
- ProteinModelPortal:   Q04LL3
- SMR:   Q04LL3
- STRING:   Q04LL3
- EnsemblBacteria:   EBSTRT00000019732
- GeneID:   4442325
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0586
- eggNOG:   COG1234
- GeneTree:   EBGT00050000027751
- HOGENOM:   HBG648406
- OMA:   HSTSTMA
- ProtClustDB:   PRK00055
- HAMAP:   MF_01818
- InterPro:   IPR001279
- InterPro:   IPR013471
- SMART:   SM00849
- TIGRFAMs:   TIGR02651

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.26.11

Molecular weight: Translated: 34140; Mature: 34140

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQNRILETTIRPRKVSKIF
CCEEEEECCCCCCHHHCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEHHCCCCCEEEEEE
ITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFVLTSLRVSGSRLPYRIHFH
EEEECCCEEECCCCHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCEEEEEE
EFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLEGTLDAEKLKAAGVPFGPLFG
ECCHHHHHHHHCCCCEEEEHHHHCCEEEEHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHH
KIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTRKTGASVRLAVNADVLVHESTYG
CCCCCCEEEEECCCCEEEHHCCCCCCCCEEEEEECCCCCCCCEEEEEEECEEEEEECCCC
KGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHISARFLSKDISKLKKDAATIFENVHV
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKDLEEVEI
HHHHHHHCC
>Mature Secondary Structure
MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQNRILETTIRPRKVSKIF
CCEEEEECCCCCCHHHCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEHHCCCCCEEEEEE
ITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFVLTSLRVSGSRLPYRIHFH
EEEECCCEEECCCCHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCEEEEEE
EFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLEGTLDAEKLKAAGVPFGPLFG
ECCHHHHHHHHCCCCEEEEHHHHCCEEEEHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHH
KIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTRKTGASVRLAVNADVLVHESTYG
CCCCCCEEEEECCCCEEEHHCCCCCCCCEEEEEECCCCCCCCEEEEEEECEEEEEECCCC
KGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHISARFLSKDISKLKKDAATIFENVHV
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKDLEEVEI
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA