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The map label for this gene is murI

Identifier: 116516596

GI number: 116516596

Start: 1672430

End: 1673224

Strand: Reverse

Name: murI

Synonym: SPD_1661

Alternate gene names: 116516596

Gene position: 1673224-1672430 (Counterclockwise)

Preceding gene: 116516372

Following gene: 116515496

Centisome position: 81.78

GC content: 48.3

Gene sequence:

>795_bases
ATGGATAATCGACCAATTGGTTTTTTGGATTCGGGTGTCGGGGGCTTGACCGTTGTGCGCGAGCTCATGCGCCAGCTTCC
CCATGAAGAAATCGTCTATATTGGAGATTCGGCGCGGGCGCCCTATGGCCCCCGTCCTGCTGAGCAAATTCGTGAATATA
CTTGGCAGCTGGTCAACTTTCTCTTGACCAAGGATGTCAAAATGATTGTCATTGCTTGTAACACTGCGACTGCGGTTGTC
TGGGAAGAAATCAAGGCTCAACTAGATATTCCTGTCTTGGGTGTAATTTTGCCAGGAGCTTCGGCAGCCATCAAGTCCAG
TCAAGGTGGGAAAATCGGAGTGATTGGAACGCCCATGACGGTACAATCAGACATATACCGTCAGAAAATCCATGATCTGG
ATCCCGACTTACAGGTGGAGAGCTTGGCCTGTCCCAAGTTTGCTCCCTTGGTTGAGTCAGGTGCCCTGTCAACCAGTGTT
ACCAAGAAGGTGGTCTATGAAACCCTGCGTCCCTTGGTTGGAAAGGTGGATAGCCTGATTTTGGGCTGTACTCATTATCC
ACTCCTTCGCCCTATTATCCAAAATGTGATGGGGCCAAAGGTTCAGCTCATCGATAGTGGGGCAGAGTGCGTACGGGATA
TTTCAGTCTTACTCAATTATTTTGAAATCAATCGTGGTCGCGATGCTGGACCACTCCATCACCGTTTTTACACAACAGCC
AGTAGCCAAAGTTTTGCACAAATTGGTGAAGAATGGCTGGAAAAAGAGATTCATGTGGAGCATGTAGAATTATGA

Upstream 100 bases:

>100_bases
AGAGAGGTAGTTGACATTCTATCAAGCTGTCGGTCAGTTCGTTCAGTACAAGGAATCATAATGAACGATCAATCAGAAAA
AAGACTAGAAAGAAGACTGT

Downstream 100 bases:

>100_bases
CAAATAAAATTTATGAATATAAGGATGACCAGAACTGGTATGTTGGGTCTTATAGTATTTTTGGTGGCGTTAACAGTTTG
AGCGACTATAAGGCAGATTT

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGPRPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVV
WEEIKAQLDIPVLGVILPGASAAIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLLNYFEINRGRDAGPLHHRFYTTA
SSQSFAQIGEEWLEKEIHVEHVEL

Sequences:

>Translated_264_residues
MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGPRPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVV
WEEIKAQLDIPVLGVILPGASAAIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLLNYFEINRGRDAGPLHHRFYTTA
SSQSFAQIGEEWLEKEIHVEHVEL
>Mature_264_residues
MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGPRPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVV
WEEIKAQLDIPVLGVILPGASAAIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLLNYFEINRGRDAGPLHHRFYTTA
SSQSFAQIGEEWLEKEIHVEHVEL

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=216, Percent_Identity=30.5555555555556, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_STRP2 (Q04IS7)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_817111.1
- ProteinModelPortal:   Q04IS7
- SMR:   Q04IS7
- STRING:   Q04IS7
- EnsemblBacteria:   EBSTRT00000020010
- GeneID:   4441225
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1661
- eggNOG:   COG0796
- GeneTree:   EBGT00050000027649
- HOGENOM:   HBG645102
- OMA:   CGHIPYG
- ProtClustDB:   PRK00865
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 29138; Mature: 29138

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGPRPAEQIREYTWQLVNF
CCCCCCCHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
LLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASAAIKSSQGGKIGVIGTPMT
HHHCCCEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEEEECCCH
VQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSVTKKVVYETLRPLVGKVDSLI
HHHHHHHHHHHCCCCCCEEHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLLNYFEINRGRDAGPLHHRFYTTA
HCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEECC
SSQSFAQIGEEWLEKEIHVEHVEL
CCHHHHHHHHHHHHHHHCHHCCCC
>Mature Secondary Structure
MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGPRPAEQIREYTWQLVNF
CCCCCCCHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
LLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASAAIKSSQGGKIGVIGTPMT
HHHCCCEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEEEECCCH
VQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSVTKKVVYETLRPLVGKVDSLI
HHHHHHHHHHHCCCCCCEEHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLLNYFEINRGRDAGPLHHRFYTTA
HCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEECC
SSQSFAQIGEEWLEKEIHVEHVEL
CCHHHHHHHHHHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA