Click here to switch to the map view.

The map label for this gene is pgk [H]

Identifier: 116516585

GI number: 116516585

Start: 453712

End: 454908

Strand: Direct

Name: pgk [H]

Synonym: SPD_0445

Alternate gene names: 116516585

Gene position: 453712-454908 (Clockwise)

Preceding gene: 116516927

Following gene: 116515489

Centisome position: 22.17

GC content: 43.44

Gene sequence:

>1197_bases
ATGGCAAAACTTACTGTTAAAGACGTTGACTTGAAAGGTAAAAAAGTCCTCGTTCGTGTTGACTTCAACGTACCATTGAA
AGATGGCGTAATCACTAACGATAACCGTATCACAGCAGCTCTTCCAACTATTAAGTACATCATCGAACAAGGTGGACGTG
CAATTCTTTTCTCTCACCTTGGACGTGTGAAAGAAGAATCTGATAAAGCTGGTAAATCACTTGCTCCTGTAGCAGCTGAC
TTGGCAGCAAAACTTGGTCAAGATGTTGTTTTCCCAGGTGTCACTCGTGGTGCTGAATTGGAAGCGGCAATCAACGCTCT
TGAAGATGGACAAGTTCTCTTGGTTGAAAACACTCGTTACGAAGATGTTGACGGCAAGAAAGAATCTAAAAACGATCCTG
AACTTGGTAAATACTGGGCATCACTTGGAGATGGTATCTTCGTAAACGATGCATTCGGTACAGCTCACCGTGCACACGCA
TCTAACGTTGGTATCTCAGCAAACGTTGAAAAAGCAGTTGCTGGTTTCCTTCTTGAAAACGAAATTGCCTACATCCAAGA
AGCAGTTGAAACTCCAGAACGTCCATTCGTGGCTATCCTTGGTGGTTCAAAAGTTTCAGACAAGATCGGTGTTATCGAAA
ACTTGCTTGAAAAAGCTGATAAAGTCCTTATCGGTGGTGGGATGACTTACACATTCTACAAAGCACAAGGTATCGAAATC
GGTAACTCACTTGTAGAAGAAGACAAATTGGATGTTGCGAAAGCTCTTCTTGAAAAAGCAAATGGTAAATTGATCTTGCC
AGTTGACTCAAAAGAAGCTAACGCATTTGCTGGTTACACTGAAGTGCGTGACACTGAAGGTGAAGCAGTTTCTGAAGGCT
TCCTTGGTCTTGACATCGGTCCAAAATCTATCGCCAAATTTGACGAAGCTTTGACTGGTGCCAAAACAGTTGTATGGAAC
GGACCTATGGGTGTATTTGAAAACCCAGATTTCCAAGCTGGTACAATCGGTGTGATGGACGCTATCGTGAAACAACCAGG
AGTTAAATCAATCATCGGTGGTGGTGACTCAGCTGCCGCAGCGATTAACCTTGGCCGTGCAGACAAGTTCTCATGGATTA
GTACGGGTGGTGGAGCATCAATGGAACTTCTTGAAGGTAAGGTTCTTCCAGGACTTGCAGCCTTGACAGAAAAATAA

Upstream 100 bases:

>100_bases
ATAAAGATTAAATATCGAAAAATCTTGTGAAATCTTTCCTTATATTTCCAAAGTGTGATATAATAGTTTCGAATAAAATA
AATAAAGGAGTTTTTGTAAC

Downstream 100 bases:

>100_bases
GATTTTATAAACAAATCAAAGAAGAGAGGGATGAGAGTTCCTCTTTTCTTTTGCTTAAAATAAAAACGCTTCCTCTCAAC
TATTACTCATAAAAATCACC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTIKYIIEQGGRAILFSHLGRVKEESDKAGKSLAPVAAD
LAAKLGQDVVFPGVTRGAELEAAINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEKADKVLIGGGMTYTFYKAQGIEI
GNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAFAGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWN
GPMGVFENPDFQAGTIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGLAALTEK

Sequences:

>Translated_398_residues
MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTIKYIIEQGGRAILFSHLGRVKEESDKAGKSLAPVAAD
LAAKLGQDVVFPGVTRGAELEAAINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEKADKVLIGGGMTYTFYKAQGIEI
GNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAFAGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWN
GPMGVFENPDFQAGTIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGLAALTEK
>Mature_397_residues
AKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTIKYIIEQGGRAILFSHLGRVKEESDKAGKSLAPVAADL
AAKLGQDVVFPGVTRGAELEAAINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHAS
NVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEKADKVLIGGGMTYTFYKAQGIEIG
NSLVEEDKLDVAKALLEKANGKLILPVDSKEANAFAGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNG
PMGVFENPDFQAGTIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGLAALTEK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=413, Percent_Identity=46.2469733656174, Blast_Score=331, Evalue=9e-91,
Organism=Homo sapiens, GI31543397, Length=413, Percent_Identity=45.0363196125908, Blast_Score=324, Evalue=7e-89,
Organism=Escherichia coli, GI1789294, Length=399, Percent_Identity=43.1077694235589, Blast_Score=276, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17508823, Length=416, Percent_Identity=49.0384615384615, Blast_Score=344, Evalue=5e-95,
Organism=Saccharomyces cerevisiae, GI6319857, Length=415, Percent_Identity=48.1927710843374, Blast_Score=341, Evalue=1e-94,
Organism=Drosophila melanogaster, GI17136394, Length=413, Percent_Identity=46.4891041162228, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24581203, Length=417, Percent_Identity=44.3645083932854, Blast_Score=303, Evalue=1e-82,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41939; Mature: 41808

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTIKYIIEQGGRAILFSHL
CCEEEEEECCCCCCEEEEEEECCCCCCCCEEECCCEEEEHHHHHHHHHHCCCCEEEHHHH
GRVKEESDKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEAAINALEDGQVLLVENTRY
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCC
EDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHASNVGISANVEKAVAGFLLEN
CCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHH
EIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEKADKVLIGGGMTYTFYKAQGIEI
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCEEEECCCEEEEEEEECCCCC
GNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAFAGYTEVRDTEGEAVSEGFLGLDIG
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCEEECCC
PKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAGTIGVMDAIVKQPGVKSIIGGGDSAAA
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCCEE
AINLGRADKFSWISTGGGASMELLEGKVLPGLAALTEK
EEECCCCCCCEEEECCCCCCHHHHCCCCCCCHHHHCCC
>Mature Secondary Structure 
AKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTIKYIIEQGGRAILFSHL
CEEEEEECCCCCCEEEEEEECCCCCCCCEEECCCEEEEHHHHHHHHHHCCCCEEEHHHH
GRVKEESDKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEAAINALEDGQVLLVENTRY
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCC
EDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHASNVGISANVEKAVAGFLLEN
CCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHH
EIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEKADKVLIGGGMTYTFYKAQGIEI
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCEEEECCCEEEEEEEECCCCC
GNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAFAGYTEVRDTEGEAVSEGFLGLDIG
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCEEECCC
PKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAGTIGVMDAIVKQPGVKSIIGGGDSAAA
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCCEE
AINLGRADKFSWISTGGGASMELLEGKVLPGLAALTEK
EEECCCCCCCEEEECCCCCCHHHHCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA