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The map label for this gene is gmuD [H]

Identifier: 116516550

GI number: 116516550

Start: 238728

End: 240107

Strand: Direct

Name: gmuD [H]

Synonym: SPD_0247

Alternate gene names: 116516550

Gene position: 238728-240107 (Clockwise)

Preceding gene: 116515963

Following gene: 116516194

Centisome position: 11.67

GC content: 42.97

Gene sequence:

>1380_bases
ATGCTAAGATTTCCAAAGGATTTTGTCTGGGGATCCTCTACTTCTGGACCGCAGACAGAAGGACGTGTAGCTGGTGACGG
TAAGGGAGACAATCTCTGGGATTACTGGTTCCAAGTGGAGCCAAATCGTTACTATAATGGGATTGGTTCAGATAAGACAT
CGACTTTTTATGAAAATTGGGAGCGGGATATTGAGCTTTTGTTAGAGACTGGTCACACAGTCTTTCGGACTTCTATTCAG
TGGTCACGGATTTTTCCACAAGGCTGTGGAAAAGTCAACCCTCAAGGTGTGGATTTTTATCGTAAGGTCTTTGAGGCTAT
TAAGGCTAAAGGAATTCGTCTGTTAGTCAATCTCTATCATTTTGATTTACCTTTTGCCCTTCAAGAGGATGGTGATGGTT
GGGAAAATAAGGCGACAGTCTCAGCCTATGAAGACTATGCTCGTTTTTGTTTTGAGACTTATGGAGATTTAGTGGATCAA
TGGATTACCTTTAACGAGCCCATCGTTCCTGTAGAATTTGGTTATTTTTATGATGCCCATTATCCACATAAGGTGGATGC
AGAGGCAGCTGTTAAGGTAGCCTATCATACACAATTGGCCAGCAGCCGGGCTGTTAAGGCTTGCCATGAACTTTTGCCTG
ATTCCAAGATTGGGATTGTCCTCAACTTGACACCGGCTTATCCACGTAGCCAGCATCCTGCTGATGTCAAGGCAGCTCGT
ATTGCGGCCCTTTTTCAGGCCCAATCTTTCTTAGATCCATCTGTCTTGGGGACTTATCCACAGGAGTTGGTAGAAATCTT
GCATGAACACGGTCTTTTACCTGATGCTACAGAGGAAGAGTTGGAACTCATTCGTGATAATACGGTGGACTTCCTTGGTG
TGAACTACTATCAACCTTTGCGTGTTATGGCTCCTCGATTTGCTAAGCATCCAGAGAGTCCACTCTTACCAGAACATTTT
TACGAGCCTTATGTGATGCCTGGACGTAAAATCAATTCTCATCGTGGTTGGGAGATTTATGAGCAAGGGATTTATGACAT
CGCCCAAAATATCAAGGAAAATTATGGCAATATTGAGTGGATGTTGACTGAGAATGGTATGGGTGTTGAAGGGGAAGAAA
AATTCCGTCAAGATGGAATGATTCAAGATGATTACCGTATTGACTTTGTAAAAGGTCATCTTCGTGAACTTCACCGTGCC
ATTGAAGATGGTGCCAACTGTAAGGGCTACTTGATTTGGACCTTTATTGACTGCTGGTCATGGCTCAACAGCTATAAAAA
TCGCTATGGTTTGGTCGAATTAGACTTGGAAACGCAAGAACGTCGTCTGAAGAAATCAGGCCACTGGTTCAAAGAATTAA
GCGATAATAATGGATTTTAA

Upstream 100 bases:

>100_bases
AAAACTATAATCAGAAGAAAAACAAGGAAAAAATGTAACTAGTTTTTACCTTGTTTTTTCTGTATAATGGGAGAAATGAG
TAGATAAAGAGGTAAATGTT

Downstream 100 bases:

>100_bases
AAAGGACTCTGACTGATTATCCCAATCGGTCAGAGTTTTTTGCTTACAGGAGCTTAATTTGAACTATACCAATTTTTACT
CTTGACAAGTGAAAGAAACA

Product: glycosyl hydrolase, family protein 1

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRYYNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQ
WSRIFPQGCGKVNPQGVDFYRKVFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIGIVLNLTPAYPRSQHPADVKAAR
IAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDATEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHF
YEPYVMPGRKINSHRGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKGHLRELHRA
IEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWFKELSDNNGF

Sequences:

>Translated_459_residues
MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRYYNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQ
WSRIFPQGCGKVNPQGVDFYRKVFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIGIVLNLTPAYPRSQHPADVKAAR
IAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDATEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHF
YEPYVMPGRKINSHRGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKGHLRELHRA
IEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWFKELSDNNGF
>Mature_459_residues
MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRYYNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQ
WSRIFPQGCGKVNPQGVDFYRKVFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIGIVLNLTPAYPRSQHPADVKAAR
IAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDATEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHF
YEPYVMPGRKINSHRGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKGHLRELHRA
IEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWFKELSDNNGF

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=482, Percent_Identity=31.5352697095436, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI110681710, Length=473, Percent_Identity=29.8097251585624, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI13273313, Length=483, Percent_Identity=30.0207039337474, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI28376633, Length=471, Percent_Identity=28.6624203821656, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI24497614, Length=481, Percent_Identity=27.8586278586279, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1789070, Length=483, Percent_Identity=33.1262939958592, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI2367270, Length=474, Percent_Identity=33.1223628691983, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI2367174, Length=488, Percent_Identity=31.3524590163934, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17552856, Length=468, Percent_Identity=29.9145299145299, Blast_Score=214, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI17539390, Length=466, Percent_Identity=29.8283261802575, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI21356577, Length=479, Percent_Identity=29.8538622129436, Blast_Score=197, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 53196; Mature: 53196

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRYYNGIGSDKTSTFYENW
CCCCCCHHCCCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCHHCCCCCCCCCHHHHHHH
ERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRKVFEAIKAKGIRLLVNLYH
HHHHHHHHHCCCEEEEECCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHH
FDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQWITFNEPIVPVEFGYFYDAH
CCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEECCC
YPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIGIVLNLTPAYPRSQHPADVKAAR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHH
IAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDATEEELELIRDNTVDFLGVNYYQPL
HHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEECCCCCHH
RVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSHRGWEIYEQGIYDIAQNIKENYGNIEW
HHHCCHHHCCCCCCCCHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCEEE
MLTENGMGVEGEEKFRQDGMIQDDYRIDFVKGHLRELHRAIEDGANCKGYLIWTFIDCWS
EEECCCCCCCCHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHH
WLNSYKNRYGLVELDLETQERRLKKSGHWFKELSDNNGF
HHHHHHHCCCEEEEECCHHHHHHHHCCCHHHHHCCCCCC
>Mature Secondary Structure
MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRYYNGIGSDKTSTFYENW
CCCCCCHHCCCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCHHCCCCCCCCCHHHHHHH
ERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRKVFEAIKAKGIRLLVNLYH
HHHHHHHHHCCCEEEEECCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHH
FDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQWITFNEPIVPVEFGYFYDAH
CCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEECCC
YPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIGIVLNLTPAYPRSQHPADVKAAR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHH
IAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDATEEELELIRDNTVDFLGVNYYQPL
HHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEECCCCCHH
RVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSHRGWEIYEQGIYDIAQNIKENYGNIEW
HHHCCHHHCCCCCCCCHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCEEE
MLTENGMGVEGEEKFRQDGMIQDDYRIDFVKGHLRELHRAIEDGANCKGYLIWTFIDCWS
EEECCCCCCCCHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHH
WLNSYKNRYGLVELDLETQERRLKKSGHWFKELSDNNGF
HHHHHHHCCCEEEEECCHHHHHHHHCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]