Click here to switch to the map view.

The map label for this gene is fucI

Identifier: 116516456

GI number: 116516456

Start: 1959132

End: 1960898

Strand: Reverse

Name: fucI

Synonym: SPD_1986

Alternate gene names: 116516456

Gene position: 1960898-1959132 (Counterclockwise)

Preceding gene: 116515536

Following gene: 116515561

Centisome position: 95.84

GC content: 42.22

Gene sequence:

>1767_bases
ATGATTCAACATCCACGTATTGGGATTCGTCCGACTATTGATGGTCGTCGTCAAGGTGTACGCGAATCACTTGAAGTACA
AACAATGAACATGGCTAAAAGTGTGGCAGATTTGATTTCAAGCACATTGAAATATCCAGATGGGGAACCTGTGGAATGTG
TGATTTCTCCATCTACCATTGGTCGTGTTCCAGAGGCTGCAGCTTCCCATGAGTTGTTCAAAAAATCAAATGTTTGCGCA
ACAATTACAGTTACACCATGCTGGTGTTATGGTAGTGAAACTATGGATATGTCTCCAGATATTCCTCATGCTATTTGGGG
ATTTAATGGGACAGAACGCCCAGGAGCTGTCTATCTTGCAGCTGTACTAGCTTCACATGCTCAAAAAGGGATTCCAGCCT
TTGGGATTTATGGTAGAGACGTCCAGGAAGCTAGTGATACAGCTATTCCAGAAGATGTCAAAGAAAAACTTTTACGTTAT
GCGCGTGCAGCTCTTGCAACTGGCTTGATGAGAGACACTGCTTACCTATCAATGGGTAGTGTTTCGATGGGGATTGGCGG
TTCTATTGTAAATCCAGATTTCTTCCAAGAATACTTAGGAATGCGAAATGAATCGGTAGATATGACGGAGTTCACGCGCC
GTATGGACCGTGGTATTTACGACCCTGAAGAGTTCGAACGTGCGCTCAAATGGGTGAAAGAAAACGTAAAAGAAGGATTC
GACCATAACCGTGAAGACCTTGTTTTAAGCCGTGAAGAAAAAGATAGACAATGGGAATTTGTTATTAAGATGTTCATGAT
TGGACGTGACTTAATGGTTGGTAACCCAAGACTTGCTGAACTTGGTTTTGAGGAAGAAGCAGTTGGTCACCATGCTTTAG
TAGCTGGTTTCCAAGGTCAACGTCAGTGGACAGACCATTTTCCAAATGGGGACTTTATGGAAACTTTCCTCAATACTCAG
TTTGACTGGAATGGTATTCGAAAACCATTTGTATTTGCGACAGAGAATGATTCACTAAATGGTGTGTCTATGCTCTTTAA
TTATCTATTAACAAATACTCCACAAATCTTTGCTGATGTGCGTACTTATTGGAGTCCAGAGGCTGTTGAACGTGTAACAG
GATATACTTTAGAGGGTCGTGCTGCAGCTGGATTCTTACATCTAATCAACTCTGGATCTTGTACATTGGATGGTACAGGT
CAAGCTACTCGAGATGGCAAACCTGTTATGAAACCATTCTGGGAGTTGGATGAAAGTGAAGTACAGGCTATGCTTGAAAA
TACAGACTTCCCACCAGCAAACCGCGAATACTTCCGTGGAGGAGGATTCTCAACTCGTTTCTTGACGAAGGGGGATATGC
CAGTAACAATGGTACGTCTCAATCTTTTAAAAGGGGTTGGTCCAGTGCTACAAATTGCAGAAGGTTACACACTTGAACTT
CCTGAAGATGTTCACCATACTTTAGATAATCGTACAGATCCAGGATGGCCAACTACTTGGTTTGCTCCACGTTTGACAGG
AAAAGGTGCTTTCAAGTCTGTCTATGACGTCATGAATAATTGGGGAGCTAATCACGGAGCCATAACATATGGACACATTG
GAGCAGACTTGATTACCTTGGCTTCTATGTTGAGAATTCCTGTCAATATGCATAATGTACCCGAGGAAGATATCTTTAGA
CCTAAAAATTGGTCCTTATTTGGAACAGAAGATCTAGAATCAGCAGACTATCGTGCATGTCAGTTGTTGGGACCACTACA
TAAATAA

Upstream 100 bases:

>100_bases
TTCCTTTAAGTTTGGCAGAGGTAGAGGTTTATACTTATAAGTAATTTTAAAAATTATCATCGGTAGTATGAAAGAAACAG
AAAAATAAGAGGAAAATAGT

Downstream 100 bases:

>100_bases
AACTTGTTTATATAGGAGGTGAACTTACGTCCCTCCTATCCTTTTAAAAAGATTTGTTAAACAATTCACAAATAATTAAA
AACGAATACAAAAAGTAATA

Product: L-fucose isomerase

Products: NA

Alternate protein names: 6-deoxy-L-galactose isomerase; FucIase

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLKYPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCA
TITVTPCWCYGSETMDMSPDIPHAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTAIPEDVKEKLLRY
ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRGIYDPEEFERALKWVKENVKEGF
DHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNPRLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQ
FDWNGIRKPFVFATENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGYTLEGRAAAGFLHLINSGSCTLDGTG
QATRDGKPVMKPFWELDESEVQAMLENTDFPPANREYFRGGGFSTRFLTKGDMPVTMVRLNLLKGVGPVLQIAEGYTLEL
PEDVHHTLDNRTDPGWPTTWFAPRLTGKGAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFR
PKNWSLFGTEDLESADYRACQLLGPLHK

Sequences:

>Translated_588_residues
MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLKYPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCA
TITVTPCWCYGSETMDMSPDIPHAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTAIPEDVKEKLLRY
ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRGIYDPEEFERALKWVKENVKEGF
DHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNPRLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQ
FDWNGIRKPFVFATENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGYTLEGRAAAGFLHLINSGSCTLDGTG
QATRDGKPVMKPFWELDESEVQAMLENTDFPPANREYFRGGGFSTRFLTKGDMPVTMVRLNLLKGVGPVLQIAEGYTLEL
PEDVHHTLDNRTDPGWPTTWFAPRLTGKGAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFR
PKNWSLFGTEDLESADYRACQLLGPLHK
>Mature_588_residues
MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLKYPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCA
TITVTPCWCYGSETMDMSPDIPHAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTAIPEDVKEKLLRY
ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRGIYDPEEFERALKWVKENVKEGF
DHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNPRLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQ
FDWNGIRKPFVFATENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGYTLEGRAAAGFLHLINSGSCTLDGTG
QATRDGKPVMKPFWELDESEVQAMLENTDFPPANREYFRGGGFSTRFLTKGDMPVTMVRLNLLKGVGPVLQIAEGYTLEL
PEDVHHTLDNRTDPGWPTTWFAPRLTGKGAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFR
PKNWSLFGTEDLESADYRACQLLGPLHK

Specific function: Converts the aldose L-fucose into the corresponding ketose L-fuculose

COG id: COG2407

COG function: function code G; L-fucose isomerase and related proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L-fucose isomerase family

Homologues:

Organism=Escherichia coli, GI1789167, Length=588, Percent_Identity=60.7142857142857, Blast_Score=741, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FUCI_STRP2 (Q04I16)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_817372.1
- ProteinModelPortal:   Q04I16
- SMR:   Q04I16
- STRING:   Q04I16
- EnsemblBacteria:   EBSTRT00000020310
- GeneID:   4442576
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1986
- eggNOG:   COG2407
- GeneTree:   EBGT00050000029802
- HOGENOM:   HBG296690
- OMA:   PTTWFVP
- ProtClustDB:   PRK10991
- GO:   GO:0005737
- HAMAP:   MF_01254
- InterPro:   IPR015888
- InterPro:   IPR004216
- InterPro:   IPR012888
- InterPro:   IPR005763
- InterPro:   IPR009015
- InterPro:   IPR012889
- Gene3D:   G3DSA:3.20.14.10
- Gene3D:   G3DSA:3.40.50.1070
- Gene3D:   G3DSA:3.40.275.10
- TIGRFAMs:   TIGR01089

Pfam domain/function: PF02952 Fucose_iso_C; PF07881 Fucose_iso_N1; PF07882 Fucose_iso_N2; SSF50443 Fuc_isomerase_C; SSF53743 Fuc_isomerase_N

EC number: =5.3.1.25

Molecular weight: Translated: 65850; Mature: 65850

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: ACT_SITE 335-335 ACT_SITE 359-359

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLKYPDGEPVECVISPSTI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHH
GRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIPHAIWGFNGTERPGAVYLA
CCCCCHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCCCCHHHCCCCCCCCCHHHHHH
AVLASHAQKGIPAFGIYGRDVQEASDTAIPEDVKEKLLRYARAALATGLMRDTAYLSMGS
HHHHHHHHCCCCEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
VSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRGIYDPEEFERALKWVKENVKEGF
EEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
DHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNPRLAELGFEEEAVGHHALVAGFQGQ
CCCCHHEEEECCCCHHHHHHHHHHHHHCCCEEECCCCHHHCCCCHHHCCCHHEEECCCCC
RQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFATENDSLNGVSMLFNYLLTNTPQIFADV
CHHHCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHH
RTYWSPEAVERVTGYTLEGRAAAGFLHLINSGSCTLDGTGQATRDGKPVMKPFWELDESE
HHHCCHHHHHHHHCCEECCCHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHCCCHHH
VQAMLENTDFPPANREYFRGGGFSTRFLTKGDMPVTMVRLNLLKGVGPVLQIAEGYTLEL
HHHHHHCCCCCCCCCHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHCCCCEEEC
PEDVHHTLDNRTDPGWPTTWFAPRLTGKGAFKSVYDVMNNWGANHGAITYGHIGADLITL
CHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHH
ASMLRIPVNMHNVPEEDIFRPKNWSLFGTEDLESADYRACQLLGPLHK
HHHHHCCCCCCCCCHHHCCCCCCCCEECCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLKYPDGEPVECVISPSTI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHH
GRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIPHAIWGFNGTERPGAVYLA
CCCCCHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCCCCHHHCCCCCCCCCHHHHHH
AVLASHAQKGIPAFGIYGRDVQEASDTAIPEDVKEKLLRYARAALATGLMRDTAYLSMGS
HHHHHHHHCCCCEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
VSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRGIYDPEEFERALKWVKENVKEGF
EEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
DHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNPRLAELGFEEEAVGHHALVAGFQGQ
CCCCHHEEEECCCCHHHHHHHHHHHHHCCCEEECCCCHHHCCCCHHHCCCHHEEECCCCC
RQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFATENDSLNGVSMLFNYLLTNTPQIFADV
CHHHCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHH
RTYWSPEAVERVTGYTLEGRAAAGFLHLINSGSCTLDGTGQATRDGKPVMKPFWELDESE
HHHCCHHHHHHHHCCEECCCHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHCCCHHH
VQAMLENTDFPPANREYFRGGGFSTRFLTKGDMPVTMVRLNLLKGVGPVLQIAEGYTLEL
HHHHHHCCCCCCCCCHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHCCCCEEEC
PEDVHHTLDNRTDPGWPTTWFAPRLTGKGAFKSVYDVMNNWGANHGAITYGHIGADLITL
CHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHH
ASMLRIPVNMHNVPEEDIFRPKNWSLFGTEDLESADYRACQLLGPLHK
HHHHHCCCCCCCCCHHHCCCCCCCCEECCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA