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The map label for this gene is endA

Identifier: 116516431

GI number: 116516431

Start: 1761408

End: 1762232

Strand: Reverse

Name: endA

Synonym: SPD_1762

Alternate gene names: 116516431

Gene position: 1762232-1761408 (Counterclockwise)

Preceding gene: 116516278

Following gene: 116515537

Centisome position: 86.13

GC content: 43.64

Gene sequence:

>825_bases
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCCGTCGGCACCTAATAGTCCCAAAACCAATCTTAGTCAGAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Upstream 100 bases:

>100_bases
CATGGGCTATCCTGTCTCCAGCAAAATGGCAGGAATTGATTCATAAATTTACAGGAAATTAGGCTGGAGAACCAGCCTTT
TTCTAAAGATAAGGAGAAAT

Downstream 100 bases:

>100_bases
AAGATAAGCCTAAACTCCTATGTCACTTATGGATGTAGGAGTTCTTTTTACTAGTTTAAGCAGGGCTAGAACAGGTACTA
AGAAAAAATAGCAACTTCTA

Product: DNA-entry nuclease

Products: NA

Alternate protein names: Competence-specific nuclease

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Sequences:

>Translated_274_residues
MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ
>Mature_274_residues
MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Specific function: By degrading DNA that enters the cell, plays a role in the competence of cells to be transformed

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA/RNA non-specific endonuclease family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUCE_STRPN (P0A3S3)

Other databases:

- EMBL:   X54225
- EMBL:   AE005672
- PIR:   F95229
- PIR:   S10641
- RefSeq:   NP_346391.1
- EnsemblBacteria:   EBSTRT00000027225
- GeneID:   929957
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_1964
- TIGR:   SP_1964
- GeneTree:   EBGT00050000026692
- HOGENOM:   HBG698106
- OMA:   NYYETKI
- ProtClustDB:   CLSK884177
- BioCyc:   SPNE170187-1:SP_1964-MONOMER
- InterPro:   IPR018524
- InterPro:   IPR001604
- SMART:   SM00892

Pfam domain/function: PF01223 Endonuclease_NS

EC number: 3.1.30.-

Molecular weight: Translated: 29891; Mature: 29891

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS01070 NUCLEASE_NON_SPEC

Important sites: ACT_SITE 160-160

Signals:

None

Transmembrane regions:

HASH(0x1c66eb04)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQASEAPSQALAESVLTD
CCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
AVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKA
HHHHHHCCCEEECCCEEEEECCCCCCCCEEECCCCCCCCCCHHCCCHHHHHHHHHHHHHH
TRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGHLLGYALIGGLDGFDASTSNP
HHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCC
KNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
CEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCHHHHC
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ
CCCCCCCEEEEEECCCCCCCCEEEEECCCEEECH
>Mature Secondary Structure
MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQASEAPSQALAESVLTD
CCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
AVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKA
HHHHHHCCCEEECCCEEEEECCCCCCCCEEECCCCCCCCCCHHCCCHHHHHHHHHHHHHH
TRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGHLLGYALIGGLDGFDASTSNP
HHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCC
KNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
CEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCHHHHC
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ
CCCCCCCEEEEEECCCCCCCCEEEEECCCEEECH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2359120; 11463916