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The map label for this gene is rumA-2 [C]

Identifier: 116516423

GI number: 116516423

Start: 1705095

End: 1706450

Strand: Reverse

Name: rumA-2 [C]

Synonym: SPD_1704

Alternate gene names: 116516423

Gene position: 1706450-1705095 (Counterclockwise)

Preceding gene: 116515970

Following gene: 116515396

Centisome position: 83.4

GC content: 37.83

Gene sequence:

>1356_bases
ATGAATCTGAAAGTGAAACAAAAAATACCATTAAAAATCAAGCGCATGGGAATTAACGGTGAGGGAATCGGCTTTTACCA
AAAAACATTAGTCTTTGTACCAGGAGCTCTCAAAGGCGAAGATATCTATTGTCAGATTACTTCTATTAGACGCAACTTTG
TTGAAGCAAAATTACTGAAGGTCAACAAGAAGTCTAAATTTCGAATTGTGCCATCTTGTACTATTTATAATGAATGCGGA
GGCTGCCAAATCATGCACCTGCATTATGATAAGCAGCTGGAGTTCAAGACGGACTTACTTCATCAAGCGCTGAAAAAATT
TGCTCCTGCAGGATATGAAAATTATGAAATTCGTCCAACTATTGGAATGCAGGAACCAAAATATTACAGAGCTAAGTTAC
AATTTCAGACTCGAAAATTTAAAAATCAGGTCAAGGCGGGCTTATATGCACAAAACTCTCACTATTTAGTAGAGTTGAAA
GACTGCCTGGTACAAGATAAGGAAACCCAAGTGATTGCTAATCGCTTAGCAGAATTACTTACTTATCACCAGATTCCAAT
CACGGATGAGAGAAAAGTTCTAGGTGTCCGTACTATTATGGTCCGACGCGCGAGAAAGACCGGACAGGTTCAGATTATTA
TTGTTACAAACCGCCAGCTTAATTTAACTCAATTGGTAAAAGAGTTGGTTAAAGATTTCCCAGAAGTTGTGACAGTAGCT
GTTAATACAAATACAGCTAAAACCAGTGAGATATATGGTGAAAAGACAGAGATTATCTGGGGGCAAGAGAGTATTCAAGA
AGGTGTACTCAATTATGAATTTTCACTATCCCCTCGAGCTTTTTATCAACTAAATCCTGAGCAAACAGAAGTCCTCTATA
GCGAAGCAGTAAAAGCGCTGGATGTTGATAAAGAAGACCATTTGATTGACGCTTATTGTGGAGTTGGAACGATTGGATTT
GCCTTTGCAAAGAAAGTAAAAACACTCAGAGGTATGGATATTATTCCAGAAGCTATTGAAGATGCCAAGCGAAATGCTAA
AAGAATGGGATTTGACAATACTCATTATGAAGCTGGAACGGCAGAAGAGATTATTCCTCGTTGGTACAAGGAAGGCTACC
GAGCAGATGCTTTGATTGTTGACCCACCACGTACAGGTCTGGATGATAAGTTATTAGATACTATTCTTACTTATGTACCA
GAAAAAATGGTTTATATTTCTTGTAATGTTTCGACCTTGGCTCGTGATTTGGTACGCTTAGTAGAAGTCTATGATCTTCA
TTATATCCAGTCGGTCGATATGTTCCCACATACAGCTCGAACTGAAGCTGTTGTAAAATTAATAAAAAAAGTTTAA

Upstream 100 bases:

>100_bases
GTCGCCATTATCAAGCTCCATCAGATAGAGTCTTTTTTTCTTTTCAAGTTTTGTGATTTTCATAGCTCTATTATAACTCA
AAATGTGATAAGATAGGGGT

Downstream 100 bases:

>100_bases
AAAAGTAGTTGACAAAGTTTGAAAAGACTGTATAATAGTAAGAGTTGAAAATAACAACTCAGGTCCGTTGGTCAAGGGGT
TAAGACACCGCCTTTTCACG

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYCQITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECG
GCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKDFPEVVTVA
VNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSLSPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGF
AFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLLDTILTYVP
EKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKKV

Sequences:

>Translated_451_residues
MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYCQITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECG
GCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKDFPEVVTVA
VNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSLSPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGF
AFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLLDTILTYVP
EKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKKV
>Mature_451_residues
MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYCQITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECG
GCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKDFPEVVTVA
VNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSLSPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGF
AFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLLDTILTYVP
EKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKKV

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=210, Percent_Identity=32.3809523809524, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI51173878, Length=210, Percent_Identity=32.3809523809524, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI269784680, Length=277, Percent_Identity=27.0758122743682, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI269784676, Length=277, Percent_Identity=27.0758122743682, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI20149688, Length=277, Percent_Identity=27.0758122743682, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI269784678, Length=277, Percent_Identity=27.0758122743682, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1789148, Length=453, Percent_Identity=26.0485651214128, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1787083, Length=384, Percent_Identity=25, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1790403, Length=166, Percent_Identity=28.9156626506024, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71997438, Length=193, Percent_Identity=26.9430051813472, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322909, Length=432, Percent_Identity=23.1481481481481, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24666579, Length=422, Percent_Identity=26.7772511848341, Blast_Score=141, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1717_STRR6 (Q8DNH6)

Other databases:

- EMBL:   AE007317
- PIR:   C98086
- RefSeq:   NP_359309.1
- ProteinModelPortal:   Q8DNH6
- SMR:   Q8DNH6
- STRING:   Q8DNH6
- EnsemblBacteria:   EBSTRT00000015507
- GeneID:   933414
- GenomeReviews:   AE007317_GR
- KEGG:   spr:spr1717
- eggNOG:   COG2265
- GeneTree:   EBGT00050000027558
- HOGENOM:   HBG690163
- OMA:   RTVMIRR
- PhylomeDB:   Q8DNH6
- ProtClustDB:   CLSK877229
- BioCyc:   SPNE171101:SPR1717-MONOMER
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 51743; Mature: 51743

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 408-408 BINDING 283-283 BINDING 312-312 BINDING 333-333 BINDING 381-381

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYCQITSIRRNFVEAKLLK
CCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHE
VNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPT
ECCCCCEEEEECEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEC
IGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELKDCLVQDKETQVIANRLAELL
CCCCCCHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEEEHHHHHCCCHHHHHHHHHHHHH
TYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKDFPEVVTVA
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHEEEE
VNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSLSPRAFYQLNPEQTEVLYSEAVKAL
EECCCCCHHHHCCCCEEEEECHHHHHCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHH
DVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNTHYEAGT
CCCCCCCEEEHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
AEEIIPRWYKEGYRADALIVDPPRTGLDDKLLDTILTYVPEKMVYISCNVSTLARDLVRL
HHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHH
VEVYDLHYIQSVDMFPHTARTEAVVKLIKKV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYCQITSIRRNFVEAKLLK
CCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHE
VNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPT
ECCCCCEEEEECEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEC
IGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELKDCLVQDKETQVIANRLAELL
CCCCCCHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEEEHHHHHCCCHHHHHHHHHHHHH
TYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKDFPEVVTVA
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHEEEE
VNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSLSPRAFYQLNPEQTEVLYSEAVKAL
EECCCCCHHHHCCCCEEEEECHHHHHCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHH
DVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNTHYEAGT
CCCCCCCEEEHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
AEEIIPRWYKEGYRADALIVDPPRTGLDDKLLDTILTYVPEKMVYISCNVSTLARDLVRL
HHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHH
VEVYDLHYIQSVDMFPHTARTEAVVKLIKKV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11544234