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The map label for this gene is apbE [H]

Identifier: 116516413

GI number: 116516413

Start: 1318599

End: 1319522

Strand: Direct

Name: apbE [H]

Synonym: SPD_1300

Alternate gene names: 116516413

Gene position: 1318599-1319522 (Clockwise)

Preceding gene: 116515401

Following gene: 116516114

Centisome position: 64.44

GC content: 43.94

Gene sequence:

>924_bases
TTGACTCTTCGTTCACATTCTGAACGGCTAATGGGGACTACTATCACTATTTCATTAGTGGATGAGCAAGCCGATATCTT
TCTCCAAAAATCCTTTGACTTGCTCAAAGAACTTGAATACCGCTTCAACGCCAATAGTCAAGAATCTGAGTTGATGGAAA
TCAATTATCAAGCAGGAATAGCCCCCGTCACGGTTCATCCAGACCTGTTTGAGCTGATTTCACTTGGGTTAGAGCATAGC
CTAGCGCTCTCTAGCCACCTCAATATCAGCATTGGTCCCTTAATTCAAACCTGGCGTATCGGTTTTTCAGATGCCAAGGT
CGCCCAGCCTCAAGAAATTGAATCGGTGCTGCCTCTAATCAATCCTCATGGTATCGAGTTAGATTCTTCTACTTCTACTG
TGTTTTTAAAACAGAAAGGAATGAAGATCGATCTAGGTTGTTTAGCCAAAGGATACAGTGCGGATAAGGTTGCCCAATTT
CTTAGGAAAGAGGGGGTGACTTCTGCCTTGATCAATCTGGGAGGGAATATCCTGACCATTGGAAAAAATCAGGCAAGAGG
GGATAACCCATGGCAAATCGGGATTCAAGACCCAGCCAATCCTAGGGGAAATCATTTAATGACCATCCCTGTTGTCAATA
AATCTGTCGTGACTTCAGGCATTTATGAACGTCACCTGACCGTCGATGGACAAGATTACCATCACATTTTTGACAGTCAA
ACAGGATATCCTGTTGAAACGGAACTAGCGAGTCTAACAATCATCTCTGATAAATCAGTCGATGGCGAAATCTGGACGAC
TCGACTCTTTGGAGAAAGACCTGCTTCTATCCTCTGGCAAGTCGAAAGTTTGGAGGGCATCGAAGTCATCCTCATCGATA
AAGAAGGCCACCTAAGCTGTTCTTCAGGAATTCCTACTCTATAG

Upstream 100 bases:

>100_bases
TATGGTATTATAATAACATGAAAATGAAATGTAAGGGCTTCAATTTAGATGATTATTTGATATTACAACTTATTTTCTAT
AAGAGAAAGGAGTTGGTAGC

Downstream 100 bases:

>100_bases
AGACAACTGTTTCAATGGAATTTTAAAATTGTATTATGTAAAAAGAGAAAGGAAATCTCATGTTAAAACTTATTGCTATT
GTTGGAACAAATTCAAAACG

Product: thiamine biosynthesis protein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNANSQESELMEINYQAGIAPVTVHPDLFELISLGLEHS
LALSSHLNISIGPLIQTWRIGFSDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVTSGIYERHLTVDGQDYHHIFDSQ
TGYPVETELASLTIISDKSVDGEIWTTRLFGERPASILWQVESLEGIEVILIDKEGHLSCSSGIPTL

Sequences:

>Translated_307_residues
MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNANSQESELMEINYQAGIAPVTVHPDLFELISLGLEHS
LALSSHLNISIGPLIQTWRIGFSDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVTSGIYERHLTVDGQDYHHIFDSQ
TGYPVETELASLTIISDKSVDGEIWTTRLFGERPASILWQVESLEGIEVILIDKEGHLSCSSGIPTL
>Mature_306_residues
TLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNANSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSL
ALSSHLNISIGPLIQTWRIGFSDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQFL
RKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVTSGIYERHLTVDGQDYHHIFDSQT
GYPVETELASLTIISDKSVDGEIWTTRLFGERPASILWQVESLEGIEVILIDKEGHLSCSSGIPTL

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=283, Percent_Identity=25.0883392226148, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 33800; Mature: 33669

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNANSQESELMEINYQAGI
CCCCCCCHHHEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCE
APVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGFSDAKVAQPQEIESVLPLI
EEEEECCHHHHHHHHCCHHHEEECCCCCEEECCEEEEEECCCCCCCCCCHHHHHHHHCCC
NPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQFLRKEGVTSALINLGGNILTI
CCCCEEECCCCCEEEEECCCCEEEHHHCCCCCCHHHHHHHHHHCCHHHHHHHCCCCEEEE
GKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVTSGIYERHLTVDGQDYHHIFDSQ
CCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCHHHHHHCHHEEEEEECCCHHHHHCCCC
TGYPVETELASLTIISDKSVDGEIWTTRLFGERPASILWQVESLEGIEVILIDKEGHLSC
CCCCEEECEEEEEEEECCCCCCCEEEEEECCCCCCEEEEEEECCCCEEEEEEECCCCEEE
SSGIPTL
CCCCCCC
>Mature Secondary Structure 
TLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNANSQESELMEINYQAGI
CCCCCCHHHEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCE
APVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGFSDAKVAQPQEIESVLPLI
EEEEECCHHHHHHHHCCHHHEEECCCCCEEECCEEEEEECCCCCCCCCCHHHHHHHHCCC
NPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQFLRKEGVTSALINLGGNILTI
CCCCEEECCCCCEEEEECCCCEEEHHHCCCCCCHHHHHHHHHHCCHHHHHHHCCCCEEEE
GKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVTSGIYERHLTVDGQDYHHIFDSQ
CCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCHHHHHHCHHEEEEEECCCHHHHHCCCC
TGYPVETELASLTIISDKSVDGEIWTTRLFGERPASILWQVESLEGIEVILIDKEGHLSC
CCCCEEECEEEEEEEECCCCCCCEEEEEECCCCCCEEEEEEECCCCEEEEEEECCCCEEE
SSGIPTL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]