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The map label for this gene is amiD

Identifier: 116516298

GI number: 116516298

Start: 1682036

End: 1682962

Strand: Reverse

Name: amiD

Synonym: SPD_1669

Alternate gene names: 116516298

Gene position: 1682962-1682036 (Counterclockwise)

Preceding gene: 116517087

Following gene: 116515459

Centisome position: 82.25

GC content: 39.91

Gene sequence:

>927_bases
ATGTCTACAATCGATAAAGAAAAATTTCAGTTTGTAAAACGTGACGATTTTGCCTCTGAAACTATTGATGCGCCAGCATA
TTCTTACTGGAAATCAGTGTTTAAACAATTTATGAAGAAAAAATCAACTGTAGTCATGTTGGGAATCTTGGTAGCCATCA
TTTTGATAAGTTTCATCTACCCAATGTTTTCTAAGTTTGATTTCAATGATGTCAGCAAGGTAAACGACTTTAGTGTTCGT
TATATCAAGCCAAATGCGGAGCATTGGTTCGGTACTGACAGTAACGGTAAATCGCTCTTTGACGGTGTCTGGTTCGGAGC
TCGTAACTCCATCCTCATTTCTGTGATTGCGACAGTGATTAACTTGGTTATCGGTGTTTTTGTCGGTGGTATTTGGGGTA
TTTCAAAATCAGTTGACCGTGTCATGATGGAAGTTTACAACGTCATCTCAAACATCCCACCTCTTTTGATTGTTATTGTC
TTGACTTACTCAATCGGAGCTGGATTCTGGAATCTGATTTTTGCCATGAGCGTAACAACATGGATTGGTATTGCCTTCAT
GATCCGTGTGCAAATCTTGCGCTATCGTGACTTGGAATACAACTTGGCGTCACGTACTTTGGGAACACCAACCTTGAAGA
TTGTTGCCAAAAATATCATGCCTCAATTGGTATCTGTTATTGTGACAACCATGACTCAAATGCTTCCAAGCTTTATCTCA
TACGAAGCCTTCTTGTCTTTCTTCGGTCTTGGATTACCGATTACAGTGCCAAGTTTGGGTCGTTTGATTTCGGATTATTC
ACAAAACGTAACAACCAATGCTTACTTGTTCTGGATTCCATTGACAACCCTTGTCTTGGTATCCTTGTCCCTTTTCGTAG
TTGGTCAAAACTTAGCGGATGCTAGTGATCCACGTACACATAGATAG

Upstream 100 bases:

>100_bases
TCATCTTTACATGTATTTCTATCTTCTCACGTCTTTTGGGAGATATTTGGATGACTATTATTGACCCACGTATTAAATTG
ACTGAGAAAGGAGGCAAATA

Downstream 100 bases:

>100_bases
GAGTAGAAATGACAAAAGAAAAAAATGTAATTTTGACTGCTCGCGATATTGTCGTGGAATTTGACGTTCGTGACAAAGTA
TTGACAGCCATTCGCGGCGT

Product: oligopeptide ABC transporter permease AmiD

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTVVMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVR
YIKPNAEHWFGTDSNGKSLFDGVWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKNIMPQLVSVIVTTMTQMLPSFIS
YEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYLFWIPLTTLVLVSLSLFVVGQNLADASDPRTHR

Sequences:

>Translated_308_residues
MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTVVMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVR
YIKPNAEHWFGTDSNGKSLFDGVWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKNIMPQLVSVIVTTMTQMLPSFIS
YEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYLFWIPLTTLVLVSLSLFVVGQNLADASDPRTHR
>Mature_307_residues
STIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTVVMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRY
IKPNAEHWFGTDSNGKSLFDGVWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIVL
TYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKNIMPQLVSVIVTTMTQMLPSFISY
EAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYLFWIPLTTLVLVSLSLFVVGQNLADASDPRTHR

Specific function: Part of the binding-protein-dependent transport system for oligopeptides; probably responsible for the translocation of the substrate across the membrane

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1787498, Length=292, Percent_Identity=30.8219178082192, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787054, Length=289, Percent_Identity=25.2595155709343, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1788505, Length=225, Percent_Identity=29.3333333333333, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787760, Length=262, Percent_Identity=24.8091603053435, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789889, Length=231, Percent_Identity=25.974025974026, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1789964, Length=222, Percent_Identity=26.5765765765766, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMID_STRPN (P0A4M9)

Other databases:

- EMBL:   X17337
- EMBL:   AE005672
- PIR:   G95220
- PIR:   S11151
- RefSeq:   NP_346320.1
- ProteinModelPortal:   P0A4M9
- EnsemblBacteria:   EBSTRT00000025216
- GeneID:   930933
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_1889
- TIGR:   SP_1889
- GeneTree:   EBGT00050000027885
- HOGENOM:   HBG729429
- OMA:   FANYDFG
- ProtClustDB:   CLSK877024
- BioCyc:   SPNE170187-1:SP_1889-MONOMER
- InterPro:   IPR000515

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 34635; Mature: 34504

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1c41f400)-; HASH(0x1cb19cac)-; HASH(0x1bb08d38)-; HASH(0x1bef0cb8)-; HASH(0x1cd2de44)-; HASH(0x1cc81670)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTVVMLGILVAIILISFIY
CCCCCHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
PMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDGVWFGARNSILISVIATVI
HHHHCCCCCHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
NLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIVLTYSIGAGFWNLIFAMSVTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKNIMPQLVSVIVTTMTQMLPSFIS
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYLFWIPLTTLVLVSLSLFVVGQNLAD
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCC
ASDPRTHR
CCCCCCCH
>Mature Secondary Structure 
STIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTVVMLGILVAIILISFIY
CCCCHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH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CCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2352474; 11463916