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The map label for this gene is eutD [H]

Identifier: 116516290

GI number: 116516290

Start: 996994

End: 997968

Strand: Direct

Name: eutD [H]

Synonym: SPD_0985

Alternate gene names: 116516290

Gene position: 996994-997968 (Clockwise)

Preceding gene: 116516162

Following gene: 116516853

Centisome position: 48.73

GC content: 43.38

Gene sequence:

>975_bases
ATGGAAGTTTTTGAAAGTCTCAAAGCCAACCTTGTTGGTAAAAATGCTCGTATCGTTCTCCCTGAAGGGGAAGAGCCTCG
TATTCTTCAAGCAACAAAACGCTTAGTAAAAGAAACAGAAGTGATTCCTGTTTTGCTTGGAAATCCTGAAAAAATTAAAA
TTTATCTTGAAATTGAAGGAATCATGGATGGTTATGAGGTCATCGACCCTCAACATTATCCTCAATTTGAAGAAATGGTT
TCTGCCTTGGTGGAGCGTCGCAAGGGCAAAATGACTGAAGAAGATGTACGCAAGGTTTTGGTTGAAGATGTCAACTACTT
TGGTGTGATGTTGGTTTACTTGGGCTTGGTTGATGGAATGGTGTCAGGAGCGATTCACTCAACAGCTTCAACAGTTCGCC
CAGCTCTACAAATCATCAAAACTCGTCCAAATGTAACTCGTACTTCAGGAGCCTTCCTCATGGTTCGTGGTACGGAACGT
TACCTATTTGGAGACTGTGCCATTAATATCAATCCAGATGCAGAAGCCTTGGCTGAAATTGCCATCAACTCAGCAATCAC
AGCTAAGATGTTTGGCATCGAACCTAAAATTGCCATGTTGAGCTATTCTACTAAAGGTTCAGGGTTTGGTGAAAGCGTTG
ATAAGGTCGTTGAAGCAACTAAAATTGCTCACGACTTGCGTCCTGACCTTGAAATCGATGGTGAGTTGCAATTTGATGCG
GCCTTTGTTCCCGAAACTGCAGCTCTGAAAGCTCCGGGAAGTACAGTAGCTGGTCAAGCAAATGTCTTCATCTTCCCAGG
TATCGAGGCAGGAAATATCGGTTACAAGATGGCTGAACGCCTGGGTGGCTTTGCGGCTGTAGGACCTGTTTTGCAAGGTT
TAAACAAGCCAGTTAATGATCTTTCTCGTGGATGTAATGCAGATGATGTTTACAAGTTGACCCTCATCACAGCAGCTCAA
GCAGTTCATCAATAG

Upstream 100 bases:

>100_bases
CCCTTGCCGGATGATTTCAGTAACCTTATTACCCAGTTATCAACTAATACTCTATAAAAACTGTCTCAGAGTATAATTAT
TATCTTAAAGGAGAAAACTC

Downstream 100 bases:

>100_bases
TGAAAACTATAAAGTGATATACTATGCTATACTGTAGTTATGAAACTATGTACGAAAAGCACTGCCATTAATTCCTGAGA
ACTAAATTACTGATTGGTGT

Product: phosphotransacetylase

Products: NA

Alternate protein names: Phosphotransacetylase; Vegetative protein 43; VEG43 [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPVLLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMV
SALVERRKGKMTEEDVRKVLVEDVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVEATKIAHDLRPDLEIDGELQFDA
AFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYKMAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQ
AVHQ

Sequences:

>Translated_324_residues
MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPVLLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMV
SALVERRKGKMTEEDVRKVLVEDVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVEATKIAHDLRPDLEIDGELQFDA
AFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYKMAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQ
AVHQ
>Mature_324_residues
MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPVLLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMV
SALVERRKGKMTEEDVRKVLVEDVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVEATKIAHDLRPDLEIDGELQFDA
AFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYKMAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQ
AVHQ

Specific function: Conversion of acetate to acetyl-CoA; second step. [C]

COG id: COG0280

COG function: function code C; Phosphotransacetylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788635, Length=310, Percent_Identity=48.3870967741936, Blast_Score=278, Evalue=2e-76,
Organism=Escherichia coli, GI1788800, Length=307, Percent_Identity=41.3680781758958, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1788806, Length=320, Percent_Identity=31.875, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: 1420 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1250 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012147
- InterPro:   IPR004614
- InterPro:   IPR002505 [H]

Pfam domain/function: PF01515 PTA_PTB [H]

EC number: =2.3.1.8 [H]

Molecular weight: Translated: 35124; Mature: 35124

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPVLLGNPEKIKIYLEIEG
CCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCCEEEEEEEEEC
IMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVEDVNYFGVMLVYLGLVDGM
CCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTERYLFGDCAININPDAEALAEI
HHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEECCCEEEEEEEEEEECCCHHHHHHH
AINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVEATKIAHDLRPDLEIDGELQFDA
HHHHHHHHHEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCEEEECE
AFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYKMAERLGGFAAVGPVLQGLNKPVND
EECCCCCEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHH
LSRGCNADDVYKLTLITAAQAVHQ
HHCCCCCCCEEEEHHHHHHHHHCC
>Mature Secondary Structure
MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPVLLGNPEKIKIYLEIEG
CCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCCEEEEEEEEEC
IMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVEDVNYFGVMLVYLGLVDGM
CCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTERYLFGDCAININPDAEALAEI
HHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEECCCEEEEEEEEEEECCCHHHHHHH
AINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVEATKIAHDLRPDLEIDGELQFDA
HHHHHHHHHEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCEEEECE
AFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYKMAERLGGFAAVGPVLQGLNKPVND
EECCCCCEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHH
LSRGCNADDVYKLTLITAAQAVHQ
HHCCCCCCCEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9298659 [H]