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The map label for this gene is prfB [H]

Identifier: 116516272

GI number: 116516272

Start: 676001

End: 676990

Strand: Direct

Name: prfB [H]

Synonym: SPD_0658

Alternate gene names: 116516272

Gene position: 676001-676990 (Clockwise)

Preceding gene: 116516866

Following gene: 116515754

Centisome position: 33.04

GC content: 42.63

Gene sequence:

>990_bases
TTGGAAAACAAGATGACAGAACCTGATTTTTGGAACGATAATATTGCGGCCCAAAAAATGTCGCAAGAATTAAATGAATT
AAAAAACACTTACAATACCTTCCATAAGATGGAAGAGTTGCAGGATGAAGTCGAAATTTTATTGGATTTTTTGGCTGAAG
ACGAGTCAGTGCATGATGAACTGGTAGCGCAGTTAGCCGAACTTGATAAGATAATGACCAGCTACGAGATGACTCTACTC
TTGTCAGAACCTTATGACCACAACAATGCCATCTTGGAAATCCATCCAGGTTCTGGTGGTACTGAGGCGCAGGACTGGGG
TGATATGTTGCTTCGTATGTATACTCGTTATGGTAATGCTAAAGGCTTTAAAGTGGAAGTGTTGGATTACCAAGCAGGTG
ATGAGGCTGGTATTAAGTCGGTAACTTTATCATTTGAAGGGCCTAATGCCTATGGTCTCCTCAAGTCAGAAATGGGTGTT
CACCGCTTAGTGCGAATCTCACCATTTGACTCTGCCAAACGTCGCCATACCTCTTTCACATCTGTAGAAGTGATGCCAGA
ATTGGATGATACTATTGAAGTGGAAATCCGTGAAGATGATATCAAGATGGATACCTTCCGTTCAGGTGGTGCCGGTGGAC
AAAACGTCAATAAGGTTTCAACAGGTGTACGTTTAACCTACATTCCAACTGGAATTGTTGTCCAATCAACAGTGGATCGT
ACCCAGTATGGAAATAGAGATCGCGCCATGAAGATGTTGCAGGCTAAGCTCTATCAAATGGAGCAAGAGAAGAAGGCTGC
GGAGGTAGATTCTCTCAAAGGTGAGAAAAAGGAGATCACTTGGGGAAGCCAAATCCGTTCTTATGTCTTCACGCCTTATA
CTATGGTAAAAGATCACCGAACTAGCTTTGAGGTTGCTCAGGTAGATAAGGTTATGGATGGGGACCTAGATGGTTTTATC
GATGCTTATCTCAAGTGGCGAATTAGCTAA

Upstream 100 bases:

>100_bases
ATTTCAGTAATTCGTCAGAAAATTGACGCAAATCGTGAAAAATTAGCTTCTTTCAGGGGGTCTCTTTGACCTCGAAGGGC
TAGAGGAAGAGATTGCCATC

Downstream 100 bases:

>100_bases
GATAGAAAGGAACTCACATGTCAATTATTGAAATGAGAGATGTCGTTAAAAAATACGACAACGGAACGACTGCTCTACGC
GGTGTTTCGGTTAGCGTTCA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MENKMTEPDFWNDNIAAQKMSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAELDKIMTSYEMTLL
LSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKVEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGV
HRLVRISPFDSAKRRHTSFTSVEVMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTYIPTGIVVQSTVDR
TQYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDHRTSFEVAQVDKVMDGDLDGFI
DAYLKWRIS

Sequences:

>Translated_329_residues
MENKMTEPDFWNDNIAAQKMSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAELDKIMTSYEMTLL
LSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKVEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGV
HRLVRISPFDSAKRRHTSFTSVEVMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTYIPTGIVVQSTVDR
TQYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDHRTSFEVAQVDKVMDGDLDGFI
DAYLKWRIS
>Mature_329_residues
MENKMTEPDFWNDNIAAQKMSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAELDKIMTSYEMTLL
LSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKVEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGV
HRLVRISPFDSAKRRHTSFTSVEVMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTYIPTGIVVQSTVDR
TQYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDHRTSFEVAQVDKVMDGDLDGFI
DAYLKWRIS

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=317, Percent_Identity=33.4384858044164, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI166795303, Length=312, Percent_Identity=33.0128205128205, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI166795305, Length=213, Percent_Identity=32.8638497652582, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI2367172, Length=324, Percent_Identity=46.6049382716049, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1787462, Length=310, Percent_Identity=34.1935483870968, Blast_Score=181, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17542784, Length=298, Percent_Identity=30.2013422818792, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=330, Percent_Identity=31.8181818181818, Blast_Score=144, Evalue=1e-35,
Organism=Drosophila melanogaster, GI19921226, Length=265, Percent_Identity=32.0754716981132, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37506; Mature: 37506

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKMTEPDFWNDNIAAQKMSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LVAQLAELDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNA
HHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
KGFKVEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFT
CCEEEEEEEECCCCCCCCEEEEEEECCCCCEEEHHHHCCHHHEEEECCCCHHHHHCCCCC
SVEVMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTYIPTGIVVQSTVDR
EEEECCCCCCEEEEEEECCCCEEECCCCCCCCCCCCHHHHCCEEEEEECCCEEEECHHHH
TQYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDHR
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHCCC
TSFEVAQVDKVMDGDLDGFIDAYLKWRIS
CCHHHHHHHHHHCCCHHHHHHHHHEEECC
>Mature Secondary Structure
MENKMTEPDFWNDNIAAQKMSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LVAQLAELDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNA
HHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
KGFKVEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFT
CCEEEEEEEECCCCCCCCEEEEEEECCCCCEEEHHHHCCHHHEEEECCCCHHHHHCCCCC
SVEVMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTYIPTGIVVQSTVDR
EEEECCCCCCEEEEEEECCCCEEECCCCCCCCCCCCHHHHCCEEEEEECCCEEEECHHHH
TQYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDHR
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHCCC
TSFEVAQVDKVMDGDLDGFIDAYLKWRIS
CCHHHHHHHHHHCCCHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA