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The map label for this gene is metG

Identifier: 116516229

GI number: 116516229

Start: 703998

End: 705995

Strand: Direct

Name: metG

Synonym: SPD_0689

Alternate gene names: 116516229

Gene position: 703998-705995 (Clockwise)

Preceding gene: 116516885

Following gene: 116517058

Centisome position: 34.41

GC content: 42.29

Gene sequence:

>1998_bases
ATGTCTGAAAAAAATTTTTATATTACAACGCCGATTTACTATCCATCTGGGAAACTTCATATCGGTTCTGCCTACACAAC
TATCGCATGTGATGTCCTAGCACGTTACAAACGCCTGATGGGCTACGATGTATTTTATCTGACAGGTCTTGATGAACATG
GTCAGAAAATCCAGCAGAAAGCGGAAGAAGCTGGTATTACACCTCAAGCCTATGTTGATGGAATGGCGGTTGGAGTTAAA
GAACTCTGGCAATTACTAGATATCTCATACGATAAATTTATCCGTACAACTGATGACTACCATGAAAAAGTTGTCGCACA
AGTCTTTGAACGCTTACTTGCTCAAGATGATATCTACTTGGGTGAATACTCTGGTTGGTATTCAGTATCAGACGAAGAAT
TCTTTACAGAAAGCCAGCTGGCAGAAGTTTTCCGTGATGAAGCTGGAAATGTGACTGGTGGTATTGCTCCATCAGGTCAT
GAGGTTGAATGGGTTTCTGAAGAATCATACTTCCTTCGCCTTAGCAAATACCAAGACCGTTTGGTCGAATTTTTCAAAGC
TCATCCTGAATTTATCACGCCAGATGGTCGCCTTAATGAAATGCTACGCAACTTCATCGAGCCAGGCTTGGAAGATTTGG
CGGTATCTCGTACAACCTTTACATGGGGAGTGCCTGTCCCATCAAATCCAAAACACGTTGTCTACGTTTGGATTGATGCC
CTTCTTAACTATGCGACAGCTCTTGGCTACGCTCAAGACGAACATGGTAACTTTGACAAGTTCTGGAATGGAACAGTCTT
CCATATGGTAGGAAAAGATATCCTTCGCTTCCACTCTATCTACTGGCCAATCCTTCTTATGATGTTGGATGTTAAATTAC
CTGATCGTTTGATTGCCCATGGTTGGTTTGTCATGAAAGACGGAAAAATGTCTAAGTCAAAAGGGAATGTCGTTTACCCT
GAAATGTTGGTAGAGCGTTATGGACTAGATCCACTTCGTTACTACCTCATGCGTAACCTTCCAGTTGGTTCAGACGGAAC
CTTTACTCCTGAAGACTATGTCGGCCGTATCAACTATGAATTGGCTAATGACCTTGGGAACCTCCTTAACCGTACGGTTT
CCATGATTAATAAGTACTTTGATGGACAAATCCCTGCCTATGTAGAAGGTGTGACTGAATTTGATCATGTTCTTGCTGAG
GTTGCAGAACAATCAATCGCAGACTTCCATACACACATGGAAGCAGTTGACTATCCACGTGCGCTTGAAGCAGTCTGGAC
TCTGATCTCTCGTACCAATAAATACATCGATGAGACCGCACCATGGGTCTTGGCCAAGGATGAAGCTCTTCGTGACCAAT
TGGCAAGTGTCATGAGCCACTTGGCAGCCAGCATTCGTGTAGTTGCTCACTTGATTGAACCATTTATGATGGAAACTAGT
CGTGCAGTTTTGACTCAACTTGGTTTGGAAGAAGTTTCTAGTCTTGAAAACTTGAGTTTGGCTGACTTCCCAGCAGATGT
GACTGTAGTTGCCAAAGGAACACCTATCTTTCCACGTCTAAATATGGAAGAAGAAATCGCCTATATCAAGGAACAAATGG
AAGGCAATAAACCAGCAGTCGAAAAAGAATGGAATCCGGACGAAGTTGAGCTCAAACTAAACAAGGATGAAATCAAGTTT
GAAGACTTTGACAAGGTTGAAATCCGTGTCGCAGAAGTCAAAGAAGTGTCTAAAGTAGAAGGTTCAGATAAGTTGCTTCA
ATTCCGCTTGGATGCTGGTGATGGAGAAGATCGTCAGATTCTTTCAGGAATTGCAAAATACTATCCAAATGAACAAGAAT
TGGTCGGCAAGAAAGTTCAAATCGTTGCTAACCTCAAACCACGTAAAATGATGAAAAAATATGTCAGCCAGGGTATGATT
CTCTCAGCTGAACATGATGGCAAATTAACCCTTCTCACAGTTGATCCAGCTGTACCAAATGGAAGTGTGATTGGGTAA

Upstream 100 bases:

>100_bases
TATCTTGTTTTTGTATGGATTTCCCTATCGAAATTCATTAAAAATGTGATATAATGAAGTGTTAGAAAAACAGGTTTCAT
TTGCCTGGAAAGGAAAAATT

Downstream 100 bases:

>100_bases
AAGTAGACAGGACTAGTTCAGGCTAGTTCTGTTTTTTTATTTATACTCAATGAAAATCAAAGAGCAAACTAGGAAGCTAG
CCGCAGGTTGCTCAAAACAC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 665; Mature: 664

Protein sequence:

>665_residues
MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDVFYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVK
ELWQLLDISYDKFIRTTDDYHEKVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRTTFTWGVPVPSNPKHVVYVWIDA
LLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYP
EMLVERYGLDPLRYYLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVTEFDHVLAE
VAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEALRDQLASVMSHLAASIRVVAHLIEPFMMETS
RAVLTQLGLEEVSSLENLSLADFPADVTVVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKF
EDFDKVEIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVANLKPRKMMKKYVSQGMI
LSAEHDGKLTLLTVDPAVPNGSVIG

Sequences:

>Translated_665_residues
MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDVFYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVK
ELWQLLDISYDKFIRTTDDYHEKVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRTTFTWGVPVPSNPKHVVYVWIDA
LLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYP
EMLVERYGLDPLRYYLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVTEFDHVLAE
VAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEALRDQLASVMSHLAASIRVVAHLIEPFMMETS
RAVLTQLGLEEVSSLENLSLADFPADVTVVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKF
EDFDKVEIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVANLKPRKMMKKYVSQGMI
LSAEHDGKLTLLTVDPAVPNGSVIG
>Mature_664_residues
SEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDVFYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKE
LWQLLDISYDKFIRTTDDYHEKVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGHE
VEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRTTFTWGVPVPSNPKHVVYVWIDAL
LNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPE
MLVERYGLDPLRYYLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVTEFDHVLAEV
AEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEALRDQLASVMSHLAASIRVVAHLIEPFMMETSR
AVLTQLGLEEVSSLENLSLADFPADVTVVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFE
DFDKVEIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVANLKPRKMMKKYVSQGMIL
SAEHDGKLTLLTVDPAVPNGSVIG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI24308436, Length=507, Percent_Identity=34.1222879684418, Blast_Score=282, Evalue=7e-76,
Organism=Homo sapiens, GI14043022, Length=579, Percent_Identity=26.7702936096718, Blast_Score=183, Evalue=5e-46,
Organism=Escherichia coli, GI1788432, Length=695, Percent_Identity=28.6330935251799, Blast_Score=265, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI212645096, Length=504, Percent_Identity=31.9444444444444, Blast_Score=258, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI71992781, Length=396, Percent_Identity=30.8080808080808, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17540794, Length=575, Percent_Identity=25.0434782608696, Blast_Score=166, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6321610, Length=524, Percent_Identity=31.1068702290076, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6321704, Length=522, Percent_Identity=24.5210727969349, Blast_Score=151, Evalue=3e-37,
Organism=Drosophila melanogaster, GI21358483, Length=500, Percent_Identity=32.6, Blast_Score=259, Evalue=5e-69,
Organism=Drosophila melanogaster, GI24655311, Length=551, Percent_Identity=25.7713248638838, Blast_Score=165, Evalue=9e-41,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_STRPN (P67580)

Other databases:

- EMBL:   AE005672
- PIR:   D95091
- RefSeq:   NP_345285.1
- ProteinModelPortal:   P67580
- EnsemblBacteria:   EBSTRT00000025729
- GeneID:   930738
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_0788
- TIGR:   SP_0788
- GeneTree:   EBGT00050000027084
- HOGENOM:   HBG721667
- OMA:   APHIGHA
- ProtClustDB:   PRK12267
- BioCyc:   SPNE170187-1:SP_0788-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_01228
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 75652; Mature: 75521

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD; PS00013 PROKAR_LIPOPROTEIN

Important sites: BINDING 312-312

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDVFYLTGLDEHGQKIQQK
CCCCCEEEEECEEECCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
AEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHEKVVAQVFERLLAQDDIYL
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEE
GEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGHEVEWVSEESYFLRLSKYQDR
ECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHH
LVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRTTFTWGVPVPSNPKHVVYVWIDA
HHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHEECEEEEECCCCCCCCCEEEEEHHHH
LLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDVKLPDRLIAH
HHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
GWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRYYLMRNLPVGSDGTFTPEDYVGRINYE
CEEEEECCCCCCCCCCEECHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHEHHH
LANDLGNLLNRTVSMINKYFDGQIPAYVEGVTEFDHVLAEVAEQSIADFHTHMEAVDYPR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ALEAVWTLISRTNKYIDETAPWVLAKDEALRDQLASVMSHLAASIRVVAHLIEPFMMETS
HHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAVLTQLGLEEVSSLENLSLADFPADVTVVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAV
HHHHHHHCHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCC
EKEWNPDEVELKLNKDEIKFEDFDKVEIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQI
CCCCCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCEEEEEECCCCCCHHHH
LSGIAKYYPNEQELVGKKVQIVANLKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPN
HHHHHHHCCCHHHHCCCCEEEEECCCHHHHHHHHHHCCEEEEECCCCEEEEEEECCCCCC
GSVIG
CCCCC
>Mature Secondary Structure 
SEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDVFYLTGLDEHGQKIQQK
CCCCEEEEECEEECCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
AEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHEKVVAQVFERLLAQDDIYL
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEE
GEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGHEVEWVSEESYFLRLSKYQDR
ECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHH
LVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRTTFTWGVPVPSNPKHVVYVWIDA
HHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHEECEEEEECCCCCCCCCEEEEEHHHH
LLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDVKLPDRLIAH
HHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
GWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRYYLMRNLPVGSDGTFTPEDYVGRINYE
CEEEEECCCCCCCCCCEECHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHEHHH
LANDLGNLLNRTVSMINKYFDGQIPAYVEGVTEFDHVLAEVAEQSIADFHTHMEAVDYPR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ALEAVWTLISRTNKYIDETAPWVLAKDEALRDQLASVMSHLAASIRVVAHLIEPFMMETS
HHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAVLTQLGLEEVSSLENLSLADFPADVTVVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAV
HHHHHHHCHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCC
EKEWNPDEVELKLNKDEIKFEDFDKVEIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQI
CCCCCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCEEEEEECCCCCCHHHH
LSGIAKYYPNEQELVGKKVQIVANLKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPN
HHHHHHHCCCHHHHCCCCEEEEECCCHHHHHHHHHHCCEEEEECCCCEEEEEEECCCCCC
GSVIG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11463916